Restriction map for
pF1KB9872
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaI BsaWI BseAI BsiWI BsmFI
BsmI Bsp1407I BspDI BspEI BspMI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KpnI KspI MluI MroI
MslI MspA1I NaeI NarI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 112 CGCG CG^CG
AcyI 268 GACGCC G[GA]^CG[CT]C
AlwI 939 GATCC GGATC(4/5)
AlwNI 937 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 490 GGGCCC GGGCC^C
AspHI 483 GAGCTC G[AT]GC[AT]^C
BclI 119 TGATCA T^GATCA
BlpI 820 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 820 GCTGAGC GC^T[ACGT]AGC
BsaBI 120 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 268 GACGCC G[GA]^CG[CT]C
BsgI 1025 GTGCAG GTGCAG(16/14)
BsiHKAI 483 GAGCTC G[AT]GC[AT]^C
BspHI 758 TCATGA T^CATGA
BssSI 97 CACGAG CACGAG(-5/-1)
BstUI 112 CGCG CG^CG
BstXI 854 CCACCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 938 AGATCC [GA]^GATC[CT]
CelII 820 GCTGAGC GC^T[ACGT]AGC
DrdI 3 GACGGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 377 CCATGG C^C[AT][AT]GG
FbaI 119 TGATCA T^GATCA
Hin1I 268 GACGCC G[GA]^CG[CT]C
HincII 316 GTCAAC GT[CT]^[GA]AC
HindII 316 GTCAAC GT[CT]^[GA]AC
MamI 120 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 938 AGATCC [GA]^GATC[CT]
MseI 1457 TTAA T^TAA
MvnI 112 CGCG CG^CG
NcoI 377 CCATGG C^CATGG
NotI 296 GCGGCCGC GC^GGCCGC
PflMI 936 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1057 GAGTC GAGTC(4/5)
PshAI 1318 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 758 TCATGA T^CATGA
SacI 483 GAGCTC GAGCT^C
ScaI 236 AGTACT AGT^ACT
SfcI 1115 CTGTAG C^T[GA][CT]AG
SmaI 45 CCCGGG CCC^GGG
SmlI 154 CTCAAG C^T[CT][GA]AG
SphI 206 GCATGC GCATG^C
StyI 377 CCATGG C^C[AT][AT]GG
TfiI 902 GAATC G^A[AT]TC
Tru9I 1457 TTAA T^TAA
Van91I 936 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 938 AGATCC [GA]^GATC[CT]
XmaCI 45 CCCGGG C^CCGGG
XmaI 45 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 332 ACATGT A^C[GA][CT]GT
1130 ACATGT
AocI 225 CCTGAGG CC^T[ACGT]AGG
870 CCTCAGG
BalI 779 TGGCCA TGG^CCA
863 TGGCCA
BanI 598 GGCACC G^G[CT][GA]CC
808 GGCACC
BciVI 1120 GGATAC GTATCC(6/5)
1073 GTATCC
BspLU11I 332 ACATGT A^CATGT
1130 ACATGT
BsrFI 254 ACCGGC [GA]^CCGG[CT]
312 GCCGGT
BsrI 288 CCAGT ACTGG(1/-1)
1070 CCAGT
Bsu36I 225 CCTGAGG CC^T[ACGT]AGG
870 CCTCAGG
Cfr10I 254 ACCGGC [GA]^CCGG[CT]
312 GCCGGT
DraII 489 GGGGCCC [GA]G^G[ACGT]CC[CT]
929 AGGGCCT
DsaI 377 CCATGG C^C[GA][CT]GG
623 CCACGG
EagI 38 CGGCCG C^GGCCG
297 CGGCCG
EclXI 38 CGGCCG C^GGCCG
297 CGGCCG
Eco52I 38 CGGCCG C^GGCCG
297 CGGCCG
Eco81I 225 CCTGAGG CC^T[ACGT]AGG
870 CCTCAGG
EcoO109I 489 GGGGCCC [GA]G^G[ACGT]CC[CT]
929 AGGGCCT
FauI 493 CCCGC CCCGC(4/6)
917 CCCGC
HaeII 422 AGCGCC [GA]GCGC^[CT]
449 AGCGCC
HinfI 902 GAATC G^A[ACGT]TC
1057 GAGTC
MluNI 779 TGGCCA TGG^CCA
863 TGGCCA
MscI 779 TGGCCA TGG^CCA
863 TGGCCA
SapI 1264 GAAGAGC GCTCTTC(1/4)
465 GCTCTTC
SfaNI 1081 GATGC GCATC(5/9)
611 GCATC
XcmI 51 CCACCCCCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
176 CCAACACGCTGCTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AvaI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsmBI BsoBI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MnlI MspI MunI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I