Restriction map for
pF1KB9851
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BbsI BcgI BfrI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I ClaI CpoI DraII DrdI
DsaI EagI EclXI Eco47III Eco52I
EcoO109I EcoRV EcoT22I FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 801 GTAC GT^AC
AhdI 868 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 856 CCTGAGG CC^T[ACGT]AGG
ApaI 724 GGGCCC GGGCC^C
AspEI 868 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1084 GGATCC G^GATCC
BanII 724 GGGCCC G[GA]GC[CT]^C
BbvCI 313 GCTGAGG CCTCAGC(-5/-2)
BciVI 710 GTATCC GTATCC(6/5)
BclI 917 TGATCA T^GATCA
BfaI 688 CTAG C^TAG
BmyI 724 GGGCCC G[AGT]GC[ACT]^C
BsaI 175 GAGACC GGTCTC(1/5)
BsmAI 175 GAGAC GTCTC(1/5)
Bsp1286I 724 GGGCCC G[AGT]GC[ACT]^C
BsrBI 304 GAGCGG CCGCTC(-3/-3)
BsrFI 1039 ACCGGC [GA]^CCGG[CT]
BstEII 1035 GGTGACC G^GT[ACGT]ACC
BstPI 1035 GGTGACC G^GT[ACGT]ACC
BstXI 811 CCAGAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 856 CCTGAGG CC^T[ACGT]AGG
CfoI 1062 GCGC GCG^C
Cfr10I 1039 ACCGGC [GA]^CCGG[CT]
DraI 1126 TTTAAA TTT^AAA
DraIII 464 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 868 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 856 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1035 GGTGACC G^GT[ACGT]ACC
FbaI 917 TGATCA T^GATCA
HhaI 1062 GCGC GCG^C
HinP1I 1062 GCGC G^CGC
MaeI 688 CTAG C^TAG
MseI 1127 TTAA T^TAA
NspI 197 GCATGT [GA]CATG^[CT]
PflMI 186 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 874 GAGTC GAGTC(4/5)
RsaI 801 GTAC GT^AC
SmlI 274 CTCAAG C^T[CT][GA]AG
StuI 12 AGGCCT AGG^CCT
TaqI 767 TCGA T^CGA
Tru9I 1127 TTAA T^TAA
TthHB8I 767 TCGA T^CGA
Van91I 186 CCACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaII 741 GGACC G^G[AT]CC
867 GGACC
BcnI 54 CCGGG CC^[GC]GG
714 CCGGG
BglI 437 GCCTCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
992 GCCGCCAAGGC
BlpI 432 GCTGAGC GC^T[ACGT]AGC
1057 GCTAAGC
Bpu1102I 432 GCTGAGC GC^T[ACGT]AGC
1057 GCTAAGC
BstYI 672 GGATCT [GA]^GATC[CT]
1084 GGATCC
CelII 432 GCTGAGC GC^T[ACGT]AGC
1057 GCTAAGC
EcoNI 495 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
651 CCTGAACCAGG
EcoRI 5 GAATTC G^AATTC
616 GAATTC
EcoT14I 996 CCAAGG C^C[AT][AT]GG
1079 CCAAGG
FauI 309 GCGGG CCCGC(4/6)
732 GCGGG
MflI 672 GGATCT [GA]^GATC[CT]
1084 GGATCC
MslI 213 CATTCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
409 CACAATGATG
MspA1I 328 CAGCTG C[AC]G^C[GT]G
483 CAGCTG
NciI 54 CCGGG CC^[GC]GG
714 CCGGG
PvuII 328 CAGCTG CAG^CTG
483 CAGCTG
SapI 910 GAAGAGC GCTCTTC(1/4)
843 GCTCTTC
StyI 996 CCAAGG C^C[AT][AT]GG
1079 CCAAGG
TfiI 633 GATTC G^A[AT]TC
959 GAATC
XhoII 672 GGATCT [GA]^GATC[CT]
1084 GGATCC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI BalI BbvI BsaJI BseRI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MluNI MnlI MscI MspI MvaI
MwoI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I Tsp509I TspRI XcmI