Restriction map for
pF1KB9850
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BanI
BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 462 CGCG CG^CG
AccIII 1118 TCCGGA T^CCGGA
Aor51HI 710 AGCGCT AGC^GCT
AvaI 881 CTCGAG C^[CT]CG[GA]G
BfaI 1030 CTAG C^TAG
BsaWI 1118 TCCGGA [AT]^CCGG[AT]
BseAI 1118 TCCGGA T^CCGGA
BseRI 547 GAGGAG GAGGAG(10/8)
BsgI 363 CTGCAC GTGCAG(16/14)
BsmAI 960 GTCTC GTCTC(1/5)
BsmI 60 GAATGC GAATGC(1/-1)
BsoBI 881 CTCGAG C^[CT]CG[GA]G
BspEI 1118 TCCGGA T^CCGGA
BsrBI 517 CCGCTC CCGCTC(-3/-3)
BsrDI 393 CATTGC GCAATG(2/0)
BstUI 462 CGCG CG^CG
DraI 1038 TTTAAA TTT^AAA
Eco47III 710 AGCGCT AGC^GCT
EcoT14I 536 CCAAGG C^C[AT][AT]GG
FauI 516 CCCGC CCCGC(4/6)
MaeI 1030 CTAG C^TAG
MaeIII 332 GTGAC ^GT[ACGT]AC
MroI 1118 TCCGGA T^CCGGA
MslI 382 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 462 CGCG CG^CG
PaeR7I 881 CTCGAG C^TCGAG
PflMI 479 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 985 GACTC GAGTC(4/5)
SapI 519 GCTCTTC GCTCTTC(1/4)
StyI 536 CCAAGG C^C[AT][AT]GG
TaqI 882 TCGA T^CGA
TfiI 687 GAATC G^A[AT]TC
Tsp45I 332 GTGAC ^GT[GC]AC
Tsp509I 1107 AATT ^AATT
TthHB8I 882 TCGA T^CGA
Van91I 479 CCATTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 881 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AfaI 175 GTAC GT^AC
408 GTAC
AlwNI 405 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
737 CAGGCTCTG
BamHI 87 GGATCC G^GATCC
995 GGATCC
BbrPI 357 CACGTG CAC^GTG
826 CACGTG
BglII 113 AGATCT A^GATCT
581 AGATCT
BmyI 787 GGGCAC G[AGT]GC[ACT]^C
851 GTGCCC
BpmI 100 CTCCAG CTGGAG(16/14)
771 CTCCAG
BsaAI 357 CACGTG [CT]AC^GT[GA]
826 CACGTG
Bsp1286I 787 GGGCAC G[AGT]GC[ACT]^C
851 GTGCCC
DdeI 75 CTCAG C^T[ACGT]AG
578 CTCAG
DsaI 125 CCGTGG C^C[GA][CT]GG
719 CCACGG
EarI 520 CTCTTC CTCTTC(1/4)
1081 GAAGAG
EcoNI 30 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
777 CCTCCATGAGG
HindIII 838 AAGCTT A^AGCTT
1094 AAGCTT
HinfI 687 GAATC G^A[ACGT]TC
985 GACTC
Ksp632I 520 CTCTTC CTCTTC(1/4)
1081 GAAGAG
MaeII 358 ACGT A^CGT
827 ACGT
MseI 1039 TTAA T^TAA
1076 TTAA
NlaIII 471 CATG CATG^
781 CATG
PmaCI 357 CACGTG CAC^GTG
826 CACGTG
PmlI 357 CACGTG CAC^GTG
826 CACGTG
PpuMI 94 AGGACCC [GA]G^G[AT]CC[CT]
748 GGGTCCT
RsaI 175 GTAC GT^AC
408 GTAC
SmlI 109 CTCAAG C^T[CT][GA]AG
881 CTCGAG
Tru9I 1039 TTAA T^TAA
1076 TTAA
XcmI 245 CCACCGACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
896 CCACTAAAATCCTGG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI Asp700I AvaII
BalI BbvI BcnI BsaJI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboI MboII MflI MluNI MnlI
MscI MspI MvaI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
SfaNI StuI TseI TspRI XhoII
XmnI