##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BfaI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        TfiI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          170  GTATAC               GT^[AC][GT]AC
AflII         106  CTTAAG               C^TTAAG
AflIII         69  ACGTGT               A^C[GA][CT]GT
Asp700I        97  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI         132  GTATCC               GTATCC(6/5)
BcnI          506  CCCGG                CC^[GC]GG
BfrI          106  CTTAAG               C^TTAAG
BmyI          124  GGGCAC               G[AGT]GC[ACT]^C
BpmI          901  CTCCAG               CTGGAG(16/14)
BsmAI          66  GAGAC                GTCTC(1/5)
BsmBI          66  GAGACG               CGTCTC(1/5)
BsmFI         318  GGGAC                GGGAC(10/14)
Bsp1286I      124  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I       47  TGTACA               T^GTACA
BsrGI          47  TGTACA               T^GTACA
Bst1107I      170  GTATAC               GTA^TAC
BstYI         264  GGATCT               [GA]^GATC[CT]
BstZ17I       170  GTATAC               GTA^TAC
EaeI          585  TGGCCG               [CT]^GGCC[GA]
EarI          666  CTCTTC               CTCTTC(1/4)
EcoT14I       812  CCAAGG               C^C[AT][AT]GG
HapII         507  CCGG                 C^CGG
HincII         73  GTCAAC               GT[CT]^[GA]AC
HindII         73  GTCAAC               GT[CT]^[GA]AC
HindIII        55  AAGCTT               A^AGCTT
HpaII         507  CCGG                 C^CGG
Ksp632I       666  CTCTTC               CTCTTC(1/4)
MflI          264  GGATCT               [GA]^GATC[CT]
MspI          507  CCGG                 C^CGG
NciI          506  CCCGG                CC^[GC]GG
NspI          992  ACATGC               [GA]CATG^[CT]
SfaNI         211  GCATC                GCATC(5/9)
SmlI          106  CTTAAG               C^T[CT][GA]AG
SspBI          47  TGTACA               T^GTACA
StuI          942  AGGCCT               AGG^CCT
StyI          812  CCAAGG               C^C[AT][AT]GG
TaqI          281  TCGA                 T^CGA
TthHB8I       281  TCGA                 T^CGA
XhoII         264  GGATCT               [GA]^GATC[CT]
XmnI           97  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

AciI          745  CCGC                 CCGC(-3/-1)
              751  CCGC                
BsaAI         337  TACGTG               [CT]AC^GT[GA]
              605  CACGTA              
Cac8I         707  GCCAGC               GC[ACGT]^[ACGT]GC
              735  GCCAGC              
NlaIII        501  CATG                 CATG^
              993  CATG                
NlaIV         192  GGACCC               GG[ACGT]^[ACGT]CC
              318  GGGACC              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AvaII       BbvI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MvaI        
MwoI        NdeII       RsaI        Sau3AI      Sau96I      
ScrFI       Tru9I       TseI        Tsp45I      Tsp509I     
TspRI