Restriction map for
pF1KB9831
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AspI AvrII BamHI
BanI BanII BbeI BbvCI BcgI
BfaI BfrI BglI BlnI BpmI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 223 GTCTAC GT^[AC][GT]AC
AcyI 325 GGCGTC G[GA]^CG[CT]C
Alw44I 702 GTGCAC G^TGCAC
AlwI 1002 GATCC GGATC(4/5)
ApaLI 702 GTGCAC G^TGCAC
AseI 811 ATTAAT AT^TAAT
AsnI 811 ATTAAT AT^TAAT
AvaII 764 GGACC G^G[AT]CC
AviII 932 TGCGCA TGC^GCA
BalI 428 TGGCCA TGG^CCA
BbrPI 745 CACGTG CAC^GTG
BbsI 592 GTCTTC GAAGAC(2/6)
BclI 200 TGATCA T^GATCA
BglII 158 AGATCT A^GATCT
BlpI 772 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 772 GCTGAGC GC^T[ACGT]AGC
BpuAI 592 GTCTTC GAAGAC(2/6)
BsaHI 325 GGCGTC G[GA]^CG[CT]C
BseRI 15 CTCCTC GAGGAG(10/8)
BsrDI 90 CATTGC GCAATG(2/0)
BsrFI 648 GCCGGT [GA]^CCGG[CT]
BstAPI 645 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 828 GGTCACC G^GT[ACGT]ACC
BstPI 828 GGTCACC G^GT[ACGT]ACC
BstXI 999 CCAGATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 772 GCTGAGC GC^T[ACGT]AGC
Cfr10I 648 GCCGGT [GA]^CCGG[CT]
EaeI 428 TGGCCA [CT]^GGCC[GA]
EcoO65I 828 GGTCACC G^GT[ACGT]ACC
FbaI 200 TGATCA T^GATCA
FspI 932 TGCGCA TGC^GCA
HgaI 326 GCGTC GACGC(5/10)
Hin1I 325 GGCGTC G[GA]^CG[CT]C
MluNI 428 TGGCCA TGG^CCA
MscI 428 TGGCCA TGG^CCA
MseI 812 TTAA T^TAA
MspA1I 754 CAGCTG C[AC]G^C[GT]G
NcoI 179 CCATGG C^CATGG
PflMI 741 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 745 CACGTG CAC^GTG
PmlI 745 CACGTG CAC^GTG
PshBI 811 ATTAAT AT^TAAT
PvuII 754 CAGCTG CAG^CTG
SapI 192 GCTCTTC GCTCTTC(1/4)
SexAI 617 ACCTGGT A^CC[AT]GGT
SfcI 924 CTACAG C^T[GA][CT]AG
SmaI 953 CCCGGG CCC^GGG
SphI 929 GCATGC GCATG^C
Tru9I 812 TTAA T^TAA
Van91I 741 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 953 CCCGGG C^CCGGG
XmaI 953 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 129 GTAC GT^AC
632 GTAC
AspHI 79 GTGCTC G[AT]GC[AT]^C
702 GTGCAC
AvaI 82 CTCGGG C^[CT]CG[GA]G
953 CCCGGG
BciVI 248 GGATAC GTATCC(6/5)
966 GGATAC
BsaAI 103 TACGTG [CT]AC^GT[GA]
745 CACGTG
BsaBI 153 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
817 GATGCACATC
BsgI 652 GTGCAG GTGCAG(16/14)
751 GTGCAG
BsiHKAI 79 GTGCTC G[AT]GC[AT]^C
702 GTGCAC
BsoBI 82 CTCGGG C^[CT]CG[GA]G
953 CCCGGG
BsrI 245 ACTGG ACTGG(1/-1)
547 CCAGT
BstYI 158 AGATCT [GA]^GATC[CT]
1001 AGATCC
DraIII 742 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
745 CACGTGGTG
DsaI 179 CCATGG C^C[GA][CT]GG
962 CCACGG
EcoT14I 179 CCATGG C^C[AT][AT]GG
413 CCTTGG
FauI 122 CCCGC CCCGC(4/6)
389 CCCGC
HaeII 680 AGCGCC [GA]GCGC^[CT]
687 GGCGCT
MamI 153 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
817 GATGCACATC
MflI 158 AGATCT [GA]^GATC[CT]
1001 AGATCC
NlaIV 3 GGAGCC GG[ACGT]^[ACGT]CC
764 GGACCC
NspI 188 ACATGC [GA]CATG^[CT]
929 GCATGC
RsaI 129 GTAC GT^AC
632 GTAC
StyI 179 CCATGG C^C[AT][AT]GG
413 CCTTGG
XhoII 158 AGATCT [GA]^GATC[CT]
1001 AGATCC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII PleI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I Tsp509I TspRI