Restriction map for
pF1KB9827
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MslI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
TspRI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Alw44I 808 GTGCAC G^TGCAC
ApaLI 808 GTGCAC G^TGCAC
AscI 410 GGCGCGCC GG^CGCGCC
AspHI 808 GTGCAC G[AT]GC[AT]^C
AvaII 720 GGACC G^G[AT]CC
BalI 263 TGGCCA TGG^CCA
BanI 16 GGCACC G^G[CT][GA]CC
BcnI 224 CCCGG CC^[GC]GG
BfaI 370 CTAG C^TAG
BglII 347 AGATCT A^GATCT
BlpI 828 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 828 GCTCAGC GC^T[ACGT]AGC
BsaI 112 GAGACC GGTCTC(1/5)
BsgI 625 GTGCAG GTGCAG(16/14)
BsiHKAI 808 GTGCAC G[AT]GC[AT]^C
BsmAI 112 GAGAC GTCTC(1/5)
BspMI 129 GCAGGT ACCTGC(4/8)
BsrI 572 ACTGG ACTGG(1/-1)
BssHII 411 GCGCGC G^CGCGC
BstXI 185 CCAGCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 347 AGATCT [GA]^GATC[CT]
CelII 828 GCTCAGC GC^T[ACGT]AGC
DdeI 829 CTCAG C^T[ACGT]AG
DpnII 348 GATC ^GATC
EagI 398 CGGCCG C^GGCCG
EarI 793 CTCTTC CTCTTC(1/4)
EclXI 398 CGGCCG C^GGCCG
Eco52I 398 CGGCCG C^GGCCG
EcoNI 636 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 842 CCATGG C^C[AT][AT]GG
HinfI 856 GAGTC G^A[ACGT]TC
Ksp632I 793 CTCTTC CTCTTC(1/4)
MaeI 370 CTAG C^TAG
MboI 348 GATC ^GATC
MflI 347 AGATCT [GA]^GATC[CT]
MluNI 263 TGGCCA TGG^CCA
MscI 263 TGGCCA TGG^CCA
NaeI 688 GCCGGC GCC^GGC
NciI 224 CCCGG CC^[GC]GG
NcoI 842 CCATGG C^CATGG
NdeII 348 GATC ^GATC
NgoMIV 688 GCCGGC G^CCGGC
PleI 856 GAGTC GAGTC(4/5)
PpuMI 719 AGGACCT [GA]G^G[AT]CC[CT]
SapI 792 GCTCTTC GCTCTTC(1/4)
Sau3AI 348 GATC ^GATC
SfaNI 101 GCATC GCATC(5/9)
SfiI 390 GGCCGACGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 932 AGGCCT AGG^CCT
StyI 842 CCATGG C^C[AT][AT]GG
XhoII 347 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 160 GACGCC G[GA]^CG[CT]C
835 GACGCC
AvaI 582 CTCGGG C^[CT]CG[GA]G
943 CCCGAG
BglI 163 GCCGTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
391 GCCGACGCGGC
BmyI 15 GGGCAC G[AGT]GC[ACT]^C
808 GTGCAC
BpmI 280 CTGGAG CTGGAG(16/14)
673 CTGGAG
BsaHI 160 GACGCC G[GA]^CG[CT]C
835 GACGCC
BsiEI 398 CGGCCG CG[GA][CT]^CG
468 CGACCG
BsmFI 575 GGGAC GGGAC(10/14)
956 GGGAC
BsoBI 582 CTCGGG C^[CT]CG[GA]G
943 CCCGAG
Bsp1286I 15 GGGCAC G[AGT]GC[ACT]^C
808 GTGCAC
BstEII 201 GGTGACC G^GT[ACGT]ACC
558 GGTCACC
BstPI 201 GGTGACC G^GT[ACGT]ACC
558 GGTCACC
DraII 233 AGGCCCT [GA]G^G[ACGT]CC[CT]
719 AGGACCT
DsaI 164 CCGTGG C^C[GA][CT]GG
842 CCATGG
EaeI 263 TGGCCA [CT]^GGCC[GA]
398 CGGCCG
EcoO109I 233 AGGCCCT [GA]G^G[ACGT]CC[CT]
719 AGGACCT
EcoO65I 201 GGTGACC G^GT[ACGT]ACC
558 GGTCACC
HaeII 269 AGCGCC [GA]GCGC^[CT]
645 GGCGCT
Hin1I 160 GACGCC G[GA]^CG[CT]C
835 GACGCC
HphI 201 GGTGA GGTGA(8/7)
560 TCACC
MaeII 440 ACGT A^CGT
818 ACGT
MaeIII 202 GTGAC ^GT[ACGT]AC
559 GTCAC
NlaIV 16 GGCACC GG[ACGT]^[ACGT]CC
491 GGCTCC
PflMI 273 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
920 CCAGCCGCTGG
PstI 295 CTGCAG CTGCA^G
649 CTGCAG
PvuII 259 CAGCTG CAG^CTG
885 CAGCTG
SfcI 295 CTGCAG C^T[GA][CT]AG
649 CTGCAG
SgrAI 589 CACCGGCG C[GA]^CCGG[CT]G
687 CGCCGGCG
SmlI 52 CTCAAG C^T[CT][GA]AG
121 CTCAAG
TaqI 380 TCGA T^CGA
803 TCGA
Tsp45I 202 GTGAC ^GT[GC]AC
559 GTCAC
TthHB8I 380 TCGA T^CGA
803 TCGA
Van91I 273 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
920 CCAGCCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AviII BbvI BsaJI BseRI BsiYI
BslI BsrBI BsrFI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I EcoRII FauI Fnu4HI
FokI FspI HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NlaIII RsaI Sau96I ScrFI
TseI