Restriction map for
pF1KB9811
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MseI
MslI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AfaI 249 GTAC GT^AC
AgeI 406 ACCGGT A^CCGGT
AlwI 18 GATCC GGATC(4/5)
AlwNI 661 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 232 GGGCCC GGGCC^C
BanI 82 GGCGCC G^G[CT][GA]CC
BbeI 82 GGCGCC GGCGC^C
BbvCI 621 CCTCAGC CCTCAGC(-5/-2)
BsaWI 406 ACCGGT [AT]^CCGG[AT]
BsgI 613 CTGCAC GTGCAG(16/14)
BsiEI 692 CGGCCG CG[GA][CT]^CG
BsmFI 335 GGGAC GGGAC(10/14)
BspHI 74 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrFI 406 ACCGGT [GA]^CCGG[CT]
BstAPI 660 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 501 CATCC GGATG(2/0)
Cfr10I 406 ACCGGT [GA]^CCGG[CT]
DraII 231 GGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 692 CGGCCG C^GGCCG
EclXI 692 CGGCCG C^GGCCG
Eco52I 692 CGGCCG C^GGCCG
EcoO109I 231 GGGGCCC [GA]G^G[ACGT]CC[CT]
FokI 501 CATCC GGATG(9/13)
HaeII 82 GGCGCC [GA]GCGC^[CT]
HgaI 337 GACGC GACGC(5/10)
KasI 82 GGCGCC G^GCGCC
MaeII 149 ACGT A^CGT
MspA1I 661 CAGCTG C[AC]G^C[GT]G
NarI 82 GGCGCC GG^CGCC
PinAI 406 ACCGGT A^CCGGT
PvuII 661 CAGCTG CAG^CTG
RcaI 74 TCATGA T^CATGA
RsaI 249 GTAC GT^AC
SapI 115 GAAGAGC GCTCTTC(1/4)
SfiI 393 GGCCGAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 82 GGCGCC GGC^GCC
SgrAI 405 CACCGGTG C[GA]^CCGG[CT]G
TaqI 593 TCGA T^CGA
TspRI 683 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 593 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 82 GGCGCC G[GA]^CG[CT]C
337 GACGCC
AspHI 103 GAGCTC G[AT]GC[AT]^C
271 GAGCTC
AvaI 235 CCCGAG C^[CT]CG[GA]G
559 CCCGAG
BalI 401 TGGCCA TGG^CCA
578 TGGCCA
BbsI 352 GTCTTC GAAGAC(2/6)
532 GTCTTC
BglI 28 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
394 GCCGAGGTGGC
BpmI 274 CTCCAG CTGGAG(16/14)
174 CTGGAG
BpuAI 352 GTCTTC GAAGAC(2/6)
532 GTCTTC
BsaHI 82 GGCGCC G[GA]^CG[CT]C
337 GACGCC
BsiHKAI 103 GAGCTC G[AT]GC[AT]^C
271 GAGCTC
BsmAI 265 GAGAC GTCTC(1/5)
671 GAGAC
BsoBI 235 CCCGAG C^[CT]CG[GA]G
559 CCCGAG
BsrI 11 CCAGT ACTGG(1/-1)
188 CCAGT
BstXI 500 CCATCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
569 CCATCTCTCTGG
CfoI 83 GCGC GCG^C
423 GCGC
EarI 115 GAAGAG CTCTTC(1/4)
709 GAAGAG
EcoT14I 461 CCATGG C^C[AT][AT]GG
602 CCATGG
HhaI 83 GCGC GCG^C
423 GCGC
Hin1I 82 GGCGCC G[GA]^CG[CT]C
337 GACGCC
HinP1I 83 GCGC G^CGC
423 GCGC
HinfI 433 GACTC G^A[ACGT]TC
631 GACTC
HphI 373 GGTGA GGTGA(8/7)
409 GGTGA
Ksp632I 115 GAAGAG CTCTTC(1/4)
709 GAAGAG
MaeIII 374 GTGAC ^GT[ACGT]AC
410 GTGAC
MluNI 401 TGGCCA TGG^CCA
578 TGGCCA
MscI 401 TGGCCA TGG^CCA
578 TGGCCA
NcoI 461 CCATGG C^CATGG
602 CCATGG
PleI 433 GACTC GAGTC(4/5)
631 GACTC
SacI 103 GAGCTC GAGCT^C
271 GAGCTC
SfaNI 483 GATGC GCATC(5/9)
425 GCATC
StyI 461 CCATGG C^C[AT][AT]GG
602 CCATGG
Tsp45I 374 GTGAC ^GT[GC]AC
410 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EcoNI
EcoRII Fnu4HI HaeIII HapII HpaII
ItaI MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI