Restriction map for
pF1KB9810
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpuAI BsaBI BsaWI BseAI BsgI
BsiEI BsiHKAI BsmBI BsmI BsoBI
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NciI NdeI NdeII NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
Sau3AI SbfI ScaI SexAI SfiI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 380 GGCGCC G[GA]^CG[CT]C
BanI 380 GGCGCC G^G[CT][GA]CC
BbeI 380 GGCGCC GGCGC^C
BbrPI 193 CACGTG CAC^GTG
BfaI 349 CTAG C^TAG
BlpI 411 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 411 GCTGAGC GC^T[ACGT]AGC
BsaHI 380 GGCGCC G[GA]^CG[CT]C
BsaI 547 GAGACC GGTCTC(1/5)
BseRI 311 CTCCTC GAGGAG(10/8)
BsiWI 638 CGTACG C^GTACG
BsmAI 547 GAGAC GTCTC(1/5)
BspMI 151 GCAGGT ACCTGC(4/8)
BstBI 187 TTCGAA TT^CGAA
CelII 411 GCTGAGC GC^T[ACGT]AGC
CfoI 381 GCGC GCG^C
EcoNI 498 CCTTGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 380 GGCGCC [GA]GCGC^[CT]
HapII 378 CCGG C^CGG
HgaI 256 GACGC GACGC(5/10)
HhaI 381 GCGC GCG^C
Hin1I 380 GGCGCC G[GA]^CG[CT]C
HinP1I 381 GCGC G^CGC
HpaII 378 CCGG C^CGG
HphI 279 GGTGA GGTGA(8/7)
KasI 380 GGCGCC G^GCGCC
MaeI 349 CTAG C^TAG
MseI 728 TTAA T^TAA
MslI 363 CATGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 378 CCGG C^CGG
NarI 380 GGCGCC GG^CGCC
NspV 187 TTCGAA TT^CGAA
PleI 123 GACTC GAGTC(4/5)
PmaCI 193 CACGTG CAC^GTG
PmlI 193 CACGTG CAC^GTG
PpuMI 506 AGGTCCC [GA]G^G[AT]CC[CT]
SapI 202 GAAGAGC GCTCTTC(1/4)
SfoI 380 GGCGCC GGC^GCC
SfuI 187 TTCGAA TT^CGAA
TaqI 188 TCGA T^CGA
TfiI 686 GATTC G^A[AT]TC
Tru9I 728 TTAA T^TAA
TthHB8I 188 TCGA T^CGA
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AluI 206 AGCT AG^CT
410 AGCT
ApaI 142 GGGCCC GGGCC^C
647 GGGCCC
BanII 142 GGGCCC G[GA]GC[CT]^C
647 GGGCCC
BmyI 142 GGGCCC G[AGT]GC[ACT]^C
647 GGGCCC
BpmI 127 CTGGAG CTGGAG(16/14)
328 CTGGAG
BsaAI 193 CACGTG [CT]AC^GT[GA]
640 TACGTG
Bsp1286I 142 GGGCCC G[AGT]GC[ACT]^C
647 GGGCCC
Bsp1407I 266 TGTACA T^GTACA
715 TGTACA
BspLU11I 362 ACATGT A^CATGT
473 ACATGT
BsrGI 266 TGTACA T^GTACA
715 TGTACA
BsrI 618 CCAGT ACTGG(1/-1)
633 CCAGT
BssKI 103 CCAGG ^CC[ACGT]GG
275 CCAGG
BstNI 103 CCAGG CC^[AT]GG
275 CCAGG
DdeI 286 CTCAG C^T[ACGT]AG
412 CTGAG
DraII 506 AGGTCCC [GA]G^G[ACGT]CC[CT]
647 GGGCCCT
EarI 314 CTCTTC CTCTTC(1/4)
202 GAAGAG
EcoO109I 506 AGGTCCC [GA]G^G[ACGT]CC[CT]
647 GGGCCCT
EcoRII 103 CCAGG ^CC[AT]GG
275 CCAGG
HinfI 123 GACTC G^A[ACGT]TC
686 GATTC
Ksp632I 314 CTCTTC CTCTTC(1/4)
202 GAAGAG
MvaI 103 CCAGG CC^[AT]GG
275 CCAGG
MwoI 300 GCCTTTGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
584 GCTGCAGGGGC
NspI 362 ACATGT [GA]CATG^[CT]
473 ACATGT
PstI 464 CTGCAG CTGCA^G
585 CTGCAG
ScrFI 103 CCAGG CC^[ACGT]GG
275 CCAGG
SfaNI 282 GATGC GCATC(5/9)
352 GATGC
SfcI 464 CTGCAG C^T[GA][CT]AG
585 CTGCAG
SspBI 266 TGTACA T^GTACA
715 TGTACA
Tsp45I 422 GTGAC ^GT[GC]AC
490 GTCAC
Tsp509I 343 AATT ^AATT
675 AATT
TspRI 467 CAGTG CA[GC]TG[ACGT][ACGT]^
619 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AflIII AvaII BbvI
BsaJI BsiYI BslI BsmFI BstF5I
Cac8I Cfr13I DsaI EcoT14I FauI
Fnu4HI FokI HaeIII ItaI MaeII
MaeIII MboII MnlI NcoI NlaIII
NlaIV RsaI Sau96I StyI TseI