##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaWI BseAI BseRI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
Eam1105I EarI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HincII HindII HindIII HinfI
HpaI HphI KpnI Ksp632I MaeI
MaeII MamI MboII MfeI MflI
MluNI MroI MscI MseI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AfaI 33 GTAC GT^AC
AflIII 29 ACGCGT A^C[GA][CT]GT
AspHI 7 GAGCTC G[AT]GC[AT]^C
AvaI 132 CCCGGG C^[CT]CG[GA]G
AviII 404 TGCGCA TGC^GCA
BlpI 306 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 306 GCTCAGC GC^T[ACGT]AGC
BsgI 158 CTGCAC GTGCAG(16/14)
BsiEI 280 CGGCCG CG[GA][CT]^CG
BsiHKAI 7 GAGCTC G[AT]GC[AT]^C
BsoBI 132 CCCGGG C^[CT]CG[GA]G
BssHII 319 GCGCGC G^CGCGC
BssSI 664 CACGAG CACGAG(-5/-1)
BstF5I 378 CATCC GGATG(2/0)
CelII 306 GCTCAGC GC^T[ACGT]AGC
DpnII 420 GATC ^GATC
DraIII 567 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 280 CGGCCG C^GGCCG
EclXI 280 CGGCCG C^GGCCG
Eco52I 280 CGGCCG C^GGCCG
FokI 378 CATCC GGATG(9/13)
FspI 404 TGCGCA TGC^GCA
MaeIII 662 GTCAC ^GT[ACGT]AC
MboI 420 GATC ^GATC
MluI 29 ACGCGT A^CGCGT
NdeII 420 GATC ^GATC
NotI 279 GCGGCCGC GC^GGCCGC
NspI 335 GCATGC [GA]CATG^[CT]
PflMI 513 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 33 GTAC GT^AC
SacI 7 GAGCTC GAGCT^C
Sau3AI 420 GATC ^GATC
SmaI 132 CCCGGG CCC^GGG
SmlI 10 CTCAAG C^T[CT][GA]AG
SphI 335 GCATGC GCATG^C
TaqI 227 TCGA T^CGA
Tsp45I 662 GTCAC ^GT[GC]AC
TthHB8I 227 TCGA T^CGA
Van91I 513 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 132 CCCGGG C^CCGGG
XmaI 132 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwNI 466 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
514 CAGGGCCTG
ApaI 129 GGGCCC GGGCC^C
601 GGGCCC
BglI 79 GCCTACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
131 GCCCGGGCGGC
BsaI 73 GGTCTC GGTCTC(1/5)
296 GGTCTC
BsmAI 74 GTCTC GTCTC(1/5)
297 GTCTC
BsrBI 204 GAGCGG CCGCTC(-3/-3)
325 GAGCGG
EcoNI 453 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
501 CCTCAACCAGG
KspI 111 CCGCGG CCGC^GG
149 CCGCGG
NlaIII 336 CATG CATG^
456 CATG
SacII 111 CCGCGG CCGC^GG
149 CCGCGG
SfaNI 480 GATGC GCATC(5/9)
311 GCATC
TspRI 567 CACTG CA[GC]TG[ACGT][ACGT]^
658 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BanII BbeI BbvI BcnI BmyI
BsaHI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI KasI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIV Sau96I
ScrFI SfoI TseI