Restriction map for
pF1KB9804
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BamHI BbeI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BsrI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 540 GAATTT [GA]^AATT[CT]
Aor51HI 160 AGCGCT AGC^GCT
ApoI 540 GAATTT [GA]^AATT[CT]
AvaI 511 CTCGAG C^[CT]CG[GA]G
BbrPI 366 CACGTG CAC^GTG
BbsI 294 GAAGAC GAAGAC(2/6)
BbvCI 501 GCTGAGG CCTCAGC(-5/-2)
BcgI 147 CGACAACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 270 CCGGG CC^[GC]GG
BpuAI 294 GAAGAC GAAGAC(2/6)
BsaAI 366 CACGTG [CT]AC^GT[GA]
BsaI 226 GAGACC GGTCTC(1/5)
BsmAI 226 GAGAC GTCTC(1/5)
BsoBI 511 CTCGAG C^[CT]CG[GA]G
BsrBI 260 CCGCTC CCGCTC(-3/-3)
BsrDI 170 GCAATG GCAATG(2/0)
BssSI 237 CACGAG CACGAG(-5/-1)
BstEII 466 GGTCACC G^GT[ACGT]ACC
BstF5I 350 CATCC GGATG(2/0)
BstPI 466 GGTCACC G^GT[ACGT]ACC
BstXI 330 CCAGGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 323 AGATCC [GA]^GATC[CT]
CfoI 161 GCGC GCG^C
Eco47III 160 AGCGCT AGC^GCT
EcoNI 510 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 466 GGTCACC G^GT[ACGT]ACC
FokI 350 CATCC GGATG(9/13)
HaeII 160 AGCGCT [GA]GCGC^[CT]
HapII 270 CCGG C^CGG
HhaI 161 GCGC GCG^C
HinP1I 161 GCGC G^CGC
HpaII 270 CCGG C^CGG
MaeII 367 ACGT A^CGT
MflI 323 AGATCC [GA]^GATC[CT]
MseI 104 TTAA T^TAA
MspI 270 CCGG C^CGG
NciI 270 CCGGG CC^[GC]GG
NcoI 621 CCATGG C^CATGG
PaeR7I 511 CTCGAG C^TCGAG
PmaCI 366 CACGTG CAC^GTG
PmlI 366 CACGTG CAC^GTG
PstI 436 CTGCAG CTGCA^G
SfcI 436 CTGCAG C^T[GA][CT]AG
SfiI 363 GGCCACGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 511 CTCGAG C^T[CT][GA]AG
Tru9I 104 TTAA T^TAA
Tsp509I 541 AATT ^AATT
XhoI 511 CTCGAG C^TCGAG
XhoII 323 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 315 GATCC GGATC(4/5)
324 GATCC
ApaI 304 GGGCCC GGGCC^C
506 GGGCCC
BalI 600 TGGCCA TGG^CCA
624 TGGCCA
BanI 416 GGCACC G^G[CT][GA]CC
574 GGTGCC
BfaI 164 CTAG C^TAG
472 CTAG
BglI 364 GCCACGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
373 GCCGCTGTGGC
BsgI 265 CTGCAC GTGCAG(16/14)
387 CTGCAC
BsiYI 407 CCCAGGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
510 CCTCGAGGAGG
BslI 407 CCCAGGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
510 CCTCGAGGAGG
DpnII 315 GATC ^GATC
324 GATC
DsaI 212 CCACGG C^C[GA][CT]GG
621 CCATGG
EaeI 600 TGGCCA [CT]^GGCC[GA]
624 TGGCCA
EarI 130 GAAGAG CTCTTC(1/4)
199 GAAGAG
FauI 441 GCGGG CCCGC(4/6)
555 GCGGG
Ksp632I 130 GAAGAG CTCTTC(1/4)
199 GAAGAG
MaeI 164 CTAG C^TAG
472 CTAG
MboI 315 GATC ^GATC
324 GATC
MluNI 600 TGGCCA TGG^CCA
624 TGGCCA
MscI 600 TGGCCA TGG^CCA
624 TGGCCA
NdeII 315 GATC ^GATC
324 GATC
NlaIII 432 CATG CATG^
622 CATG
PleI 334 GAGTC GAGTC(4/5)
475 GAGTC
Sau3AI 315 GATC ^GATC
324 GATC
TaqI 146 TCGA T^CGA
512 TCGA
TfiI 73 GAATC G^A[AT]TC
274 GAATC
TspRI 311 CAGTG CA[GC]TG[ACGT][ACGT]^
354 CACTG
TthHB8I 146 TCGA T^CGA
512 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BseRI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MboII MnlI
MspA1I MvaI MwoI NlaIV Sau96I
ScrFI StyI TseI Tsp45I XcmI