##################################
# NO CUT ENZYMES (  225 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       HphI        KasI        
KpnI        KspI        MaeI        MaeII       MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tsp45I      
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   19 enzymes) #
###################################

AlwNI         115  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          278  CTCGGG               C^[CT]CG[GA]G
AvaII         282  GGACC                G^G[AT]CC
BanII         251  GGGCTC               G[GA]GC[CT]^C
BmyI          251  GGGCTC               G[AGT]GC[ACT]^C
BsgI           39  GTGCAG               GTGCAG(16/14)
BsmAI         129  GTCTC                GTCTC(1/5)
BsmFI         281  GGGAC                GGGAC(10/14)
BsoBI         278  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      251  GGGCTC               G[AGT]GC[ACT]^C
Cfr13I        282  GGACC                G^G[ACGT]CC
DdeI          235  CTGAG                C^T[ACGT]AG
EarI          220  GAAGAG               CTCTTC(1/4)
Ksp632I       220  GAAGAG               CTCTTC(1/4)
NlaIV         281  GGGACC               GG[ACGT]^[ACGT]CC
Sau96I        282  GGACC                G^G[ACGT]CC
SfcI          159  CTATAG               C^T[GA][CT]AG
TaqI          134  TCGA                 T^CGA
TthHB8I       134  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BbvI           41  GCAGC                GCAGC(8/12)
              120  GCTGC               
BsmI          258  GCATTC               GAATGC(1/-1)
              273  GCATTC              
Cac8I          44  GCGTGC               GC[ACGT]^[ACGT]GC
               89  GCCAGC              
DraI          142  TTTAAA               TTT^AAA
              169  TTTAAA              
Fnu4HI         41  GCAGC                GC^[ACGT]GC
              120  GCTGC               
ItaI           41  GCAGC                GC^[ACGT]GC
              120  GCTGC               
MseI          143  TTAA                 T^TAA
              170  TTAA                
Tru9I         143  TTAA                 T^TAA
              170  TTAA                
TseI           41  GCAGC                G^C[AT]GC
              120  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AcsI        ApoI        MboII       MnlI        Tsp509I