##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeIII      MamI        MboII       MfeI        
MflI        MluI        MroI        MseI        MspA1I      
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp45I      Tth111I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AlwI          624  GATCC                GGATC(4/5)
ApaI          437  GGGCCC               GGGCC^C
AspHI         513  GAGCTC               G[AT]GC[AT]^C
BalI          270  TGGCCA               TGG^CCA
BfaI          104  CTAG                 C^TAG
BglI          218  GCCGGAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         118  TACGTG               [CT]AC^GT[GA]
BsaI           49  GAGACC               GGTCTC(1/5)
BseRI          58  GAGGAG               GAGGAG(10/8)
BsiHKAI       513  GAGCTC               G[AT]GC[AT]^C
BsmI          477  GCATTC               GAATGC(1/-1)
BspMI         362  ACCTGC               ACCTGC(4/8)
DpnII         624  GATC                 ^GATC
EaeI          270  TGGCCA               [CT]^GGCC[GA]
HinfI         331  GAGTC                G^A[ACGT]TC
MaeI          104  CTAG                 C^TAG
MboI          624  GATC                 ^GATC
MluNI         270  TGGCCA               TGG^CCA
MscI          270  TGGCCA               TGG^CCA
MslI          150  CACCTCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         624  GATC                 ^GATC
PflMI         552  CCAAGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          331  GAGTC                GAGTC(4/5)
SacI          513  GAGCTC               GAGCT^C
Sau3AI        624  GATC                 ^GATC
StuI          212  AGGCCT               AGG^CCT
TaqI          135  TCGA                 T^CGA
Tsp509I        42  AATT                 ^AATT
TthHB8I       135  TCGA                 T^CGA
Van91I        552  CCAAGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI          514  AGCT                 AG^CT
              529  AGCT                
BcnI            8  CCCGG                CC^[GC]GG
              618  CCCGG               
BsmAI          49  GAGAC                GTCTC(1/5)
              248  GAGAC               
DdeI          523  CTAAG                C^T[ACGT]AG
              595  CTCAG               
FauI          566  CCCGC                CCCGC(4/6)
              578  CCCGC               
HphI          621  GGTGA                GGTGA(8/7)
              254  TCACC               
MaeII         119  ACGT                 A^CGT
              261  ACGT                
NciI            8  CCCGG                CC^[GC]GG
              618  CCCGG               
SfaNI          63  GATGC                GCATC(5/9)
              468  GATGC               
XcmI          149  CCACCTCTGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              281  CCACACTGCCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AvaII       BanII       BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HpaII       ItaI        MnlI        MspI        MvaI        
MwoI        NcoI        NlaIII      NlaIV       PpuMI       
Sau96I      ScrFI       StyI        TseI        TspRI