################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeIII MamI MboII MfeI MflI MluI MroI MseI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp45I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AlwI 624 GATCC GGATC(4/5) ApaI 437 GGGCCC GGGCC^C AspHI 513 GAGCTC G[AT]GC[AT]^C BalI 270 TGGCCA TGG^CCA BfaI 104 CTAG C^TAG BglI 218 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 118 TACGTG [CT]AC^GT[GA] BsaI 49 GAGACC GGTCTC(1/5) BseRI 58 GAGGAG GAGGAG(10/8) BsiHKAI 513 GAGCTC G[AT]GC[AT]^C BsmI 477 GCATTC GAATGC(1/-1) BspMI 362 ACCTGC ACCTGC(4/8) DpnII 624 GATC ^GATC EaeI 270 TGGCCA [CT]^GGCC[GA] HinfI 331 GAGTC G^A[ACGT]TC MaeI 104 CTAG C^TAG MboI 624 GATC ^GATC MluNI 270 TGGCCA TGG^CCA MscI 270 TGGCCA TGG^CCA MslI 150 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 624 GATC ^GATC PflMI 552 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 331 GAGTC GAGTC(4/5) SacI 513 GAGCTC GAGCT^C Sau3AI 624 GATC ^GATC StuI 212 AGGCCT AGG^CCT TaqI 135 TCGA T^CGA Tsp509I 42 AATT ^AATT TthHB8I 135 TCGA T^CGA Van91I 552 CCAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AluI 514 AGCT AG^CT 529 AGCT BcnI 8 CCCGG CC^[GC]GG 618 CCCGG BsmAI 49 GAGAC GTCTC(1/5) 248 GAGAC DdeI 523 CTAAG C^T[ACGT]AG 595 CTCAG FauI 566 CCCGC CCCGC(4/6) 578 CCCGC HphI 621 GGTGA GGTGA(8/7) 254 TCACC MaeII 119 ACGT A^CGT 261 ACGT NciI 8 CCCGG CC^[GC]GG 618 CCCGG SfaNI 63 GATGC GCATC(5/9) 468 GATGC XcmI 149 CCACCTCTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 281 CCACACTGCCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AvaII BanII BbvI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII ItaI MnlI MspI MvaI MwoI NcoI NlaIII NlaIV PpuMI Sau96I ScrFI StyI TseI TspRI