Restriction map for
pF1KB9777
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AviII
AvrII BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BsaAI
BsaBI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacII SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XbaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 2086 GTCGAC GT^[AC][GT]AC
AccIII 2320 TCCGGA T^CCGGA
AcyI 1029 GGCGCC G[GA]^CG[CT]C
ApaI 1297 GGGCCC GGGCC^C
AseI 265 ATTAAT AT^TAAT
AsnI 265 ATTAAT AT^TAAT
BamHI 2002 GGATCC G^GATCC
BanI 1029 GGCGCC G^G[CT][GA]CC
BbeI 1029 GGCGCC GGCGC^C
BclI 828 TGATCA T^GATCA
BlpI 990 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 990 GCTCAGC GC^T[ACGT]AGC
BsaHI 1029 GGCGCC G[GA]^CG[CT]C
BseAI 2320 TCCGGA T^CCGGA
BsgI 814 CTGCAC GTGCAG(16/14)
BspEI 2320 TCCGGA T^CCGGA
BssSI 1615 CACGAG CACGAG(-5/-1)
BstEII 574 GGTGACC G^GT[ACGT]ACC
BstPI 574 GGTGACC G^GT[ACGT]ACC
BstXI 848 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 990 GCTCAGC GC^T[ACGT]AGC
DraI 2158 TTTAAA TTT^AAA
DrdI 732 GACAGTCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1025 CCGTGG C^C[GA][CT]GG
EcoNI 328 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 574 GGTGACC G^GT[ACGT]ACC
EcoRI 306 GAATTC G^AATTC
EcoRV 1864 GATATC GAT^ATC
FbaI 828 TGATCA T^GATCA
Hin1I 1029 GGCGCC G[GA]^CG[CT]C
HincII 2086 GTCGAC GT[CT]^[GA]AC
HindII 2086 GTCGAC GT[CT]^[GA]AC
HphI 574 GGTGA GGTGA(8/7)
KasI 1029 GGCGCC G^GCGCC
MaeII 504 ACGT A^CGT
MroI 2320 TCCGGA T^CCGGA
NarI 1029 GGCGCC GG^CGCC
PshBI 265 ATTAAT AT^TAAT
SalI 2086 GTCGAC G^TCGAC
SfoI 1029 GGCGCC GGC^GCC
SspI 41 AATATT AAT^ATT
StuI 1881 AGGCCT AGG^CCT
XcmI 1061 CCAGGGACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwNI 832 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
1321 CAGTTGCTG
AspI 58 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
973 GACGAGGTC
BalI 1689 TGGCCA TGG^CCA
2064 TGGCCA
BsaI 955 GAGACC GGTCTC(1/5)
2030 GAGACC
BsaWI 737 TCCGGT [AT]^CCGG[AT]
2320 TCCGGA
BsmI 944 GAATGC GAATGC(1/-1)
1607 GCATTC
BspMI 648 GCAGGT ACCTGC(4/8)
1341 GCAGGT
BsrDI 293 GCAATG GCAATG(2/0)
1829 GCAATG
BsrFI 627 GCCGGC [GA]^CCGG[CT]
773 GCCGGC
Cfr10I 627 GCCGGC [GA]^CCGG[CT]
773 GCCGGC
EaeI 1689 TGGCCA [CT]^GGCC[GA]
2064 TGGCCA
MluNI 1689 TGGCCA TGG^CCA
2064 TGGCCA
MscI 1689 TGGCCA TGG^CCA
2064 TGGCCA
NaeI 627 GCCGGC GCC^GGC
773 GCCGGC
NgoMIV 627 GCCGGC G^CCGGC
773 GCCGGC
PaeR7I 805 CTCGAG C^TCGAG
1973 CTCGAG
PflFI 58 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
973 GACGAGGTC
PflMI 1320 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1628 CCAAGGTGTGG
SacI 298 GAGCTC GAGCT^C
925 GAGCTC
SapI 667 GAAGAGC GCTCTTC(1/4)
909 GAAGAGC
SmaI 94 CCCGGG CCC^GGG
1304 CCCGGG
Tth111I 58 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
973 GACGAGGTC
Van91I 1320 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1628 CCAAGGTGTGG
XhoI 805 CTCGAG C^TCGAG
1973 CTCGAG
XmaCI 94 CCCGGG C^CCGGG
1304 CCCGGG
XmaI 94 CCCGGG C^CCGGG
1304 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII