Restriction map for
pF1KB9770
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AhdI Alw44I AlwNI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaBI BseAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BsrDI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DraIII Eam1105I Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI KspI MaeI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SapI SbfI SfcI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tsp509I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 663 GTCGAC GT^[AC][GT]AC
AgeI 554 ACCGGT A^CCGGT
AspHI 162 GAGCTC G[AT]GC[AT]^C
AvaI 129 CCCGGG C^[CT]CG[GA]G
AviII 488 TGCGCA TGC^GCA
BbvCI 1173 GCTGAGG CCTCAGC(-5/-2)
BglI 335 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 745 TACGTG [CT]AC^GT[GA]
BsaI 739 GAGACC GGTCTC(1/5)
BsiEI 1157 CGGCCG CG[GA][CT]^CG
BsiHKAI 162 GAGCTC G[AT]GC[AT]^C
BsmAI 739 GAGAC GTCTC(1/5)
BsmI 141 GCATTC GAATGC(1/-1)
BsoBI 129 CCCGGG C^[CT]CG[GA]G
Bsp1407I 446 TGTACA T^GTACA
BspMI 508 ACCTGC ACCTGC(4/8)
BsrGI 446 TGTACA T^GTACA
BssHII 1110 GCGCGC G^CGCGC
BstF5I 894 CATCC GGATG(2/0)
DrdI 883 GACGACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1157 CGGCCG C^GGCCG
EarI 1306 CTCTTC CTCTTC(1/4)
EclXI 1157 CGGCCG C^GGCCG
Eco52I 1157 CGGCCG C^GGCCG
FokI 894 CATCC GGATG(9/13)
FspI 488 TGCGCA TGC^GCA
HincII 663 GTCGAC GT[CT]^[GA]AC
HindII 663 GTCGAC GT[CT]^[GA]AC
Ksp632I 1306 CTCTTC CTCTTC(1/4)
MaeIII 793 GTCAC ^GT[ACGT]AC
MseI 1127 TTAA T^TAA
NaeI 1266 GCCGGC GCC^GGC
NgoMIV 1266 GCCGGC G^CCGGC
PinAI 554 ACCGGT A^CCGGT
SacI 162 GAGCTC GAGCT^C
SalI 663 GTCGAC G^TCGAC
ScaI 563 AGTACT AGT^ACT
SexAI 887 ACCAGGT A^CC[AT]GGT
SmaI 129 CCCGGG CCC^GGG
SmlI 349 CTCAAG C^T[CT][GA]AG
SspBI 446 TGTACA T^GTACA
StuI 1202 AGGCCT AGG^CCT
Tru9I 1127 TTAA T^TAA
Tsp45I 793 GTCAC ^GT[GC]AC
TspRI 264 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 129 CCCGGG C^CCGGG
XmaI 129 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 514 CGCG CG^CG
1111 CGCG
AocI 368 CCTCAGG CC^T[ACGT]AGG
425 CCTCAGG
ApaI 183 GGGCCC GGGCC^C
216 GGGCCC
AspI 787 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
886 GACCAGGTC
BalI 587 TGGCCA TGG^CCA
977 TGGCCA
BbeI 1027 GGCGCC GGCGC^C
1368 GGCGCC
BpmI 706 CTGGAG CTGGAG(16/14)
1363 CTGGAG
BsrI 265 ACTGG ACTGG(1/-1)
561 CCAGT
BstUI 514 CGCG CG^CG
1111 CGCG
Bsu36I 368 CCTCAGG CC^T[ACGT]AGG
425 CCTCAGG
Eco81I 368 CCTCAGG CC^T[ACGT]AGG
425 CCTCAGG
HgaI 344 GCGTC GACGC(5/10)
1192 GCGTC
HinfI 661 GAGTC G^A[ACGT]TC
1135 GACTC
KasI 1027 GGCGCC G^GCGCC
1368 GGCGCC
MaeII 746 ACGT A^CGT
992 ACGT
MluNI 587 TGGCCA TGG^CCA
977 TGGCCA
MscI 587 TGGCCA TGG^CCA
977 TGGCCA
MvnI 514 CGCG CG^CG
1111 CGCG
NarI 1027 GGCGCC GG^CGCC
1368 GGCGCC
NspI 686 ACATGC [GA]CATG^[CT]
1076 ACATGC
PflFI 787 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
886 GACCAGGTC
PleI 1135 GACTC GAGTC(4/5)
661 GAGTC
PpuMI 353 AGGTCCC [GA]G^G[AT]CC[CT]
494 AGGACCT
PvuII 136 CAGCTG CAG^CTG
450 CAGCTG
SfoI 1027 GGCGCC GGC^GCC
1368 GGCGCC
Tth111I 787 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
886 GACCAGGTC
XcmI 170 CCATCAACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
242 CCACACCCACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BamHI BanI BanII BbvI
BcnI BlpI BmyI Bpu1102I BsaHI
BsaJI BsaWI BseRI BsgI BsiYI
BslI BsmFI Bsp1286I BsrBI BsrFI
BssKI BstNI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI TthHB8I
XhoII