Restriction map for
pF1KB9764
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI BspDI BspEI
BspLU11I BspMI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AclI 944 AACGTT AA^CGTT
AvaII 75 GGACC G^G[AT]CC
BanII 1148 GAGCTC G[GA]GC[CT]^C
BbvI 585 GCAGC GCAGC(8/12)
BclI 263 TGATCA T^GATCA
BfaI 1330 CTAG C^TAG
BlpI 1160 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1160 GCTTAGC GC^T[ACGT]AGC
BseRI 58 GAGGAG GAGGAG(10/8)
BsmI 1381 GAATGC GAATGC(1/-1)
Bsp1407I 95 TGTACA T^GTACA
BspHI 1377 TCATGA T^CATGA
BsrBI 80 CCGCTC CCGCTC(-3/-3)
BsrDI 2216 GCAATG GCAATG(2/0)
BsrGI 95 TGTACA T^GTACA
CelII 1160 GCTTAGC GC^T[ACGT]AGC
Cfr13I 75 GGACC G^G[ACGT]CC
FauI 79 CCCGC CCCGC(4/6)
FbaI 263 TGATCA T^GATCA
HinfI 143 GAATC G^A[ACGT]TC
MaeI 1330 CTAG C^TAG
NspI 67 ACATGC [GA]CATG^[CT]
Psp1406I 944 AACGTT AA^CGTT
PstI 583 CTGCAG CTGCA^G
RcaI 1377 TCATGA T^CATGA
SacI 1148 GAGCTC GAGCT^C
Sau96I 75 GGACC G^G[ACGT]CC
SmlI 421 CTTGAG C^T[CT][GA]AG
SspBI 95 TGTACA T^GTACA
TfiI 143 GAATC G^A[AT]TC
TseI 585 GCAGC G^C[AT]GC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 96 GTAC GT^AC
217 GTAC
AflIII 898 ACACGT A^C[GA][CT]GT
2462 ACGTGT
AlwI 927 GATCC GGATC(4/5)
251 GGATC
AspHI 1148 GAGCTC G[AT]GC[AT]^C
1484 GAGCAC
BmyI 1148 GAGCTC G[AGT]GC[ACT]^C
1484 GAGCAC
BsaWI 1354 ACCGGA [AT]^CCGG[AT]
2362 ACCGGA
BsiHKAI 1148 GAGCTC G[AT]GC[AT]^C
1484 GAGCAC
BsiYI 12 CCAGGGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
80 CCGCTCAGAGG
BslI 12 CCAGGGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
80 CCGCTCAGAGG
BsmFI 15 GGGAC GGGAC(10/14)
101 GGGAC
Bsp1286I 1148 GAGCTC G[AGT]GC[ACT]^C
1484 GAGCAC
BstXI 235 CCAGATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2433 CCATACTGGTGG
Cac8I 2085 GCTTGC GC[ACGT]^[ACGT]GC
2498 GCAAGC
HaeIII 132 GGCC GG^CC
418 GGCC
HapII 1355 CCGG C^CGG
2363 CCGG
HpaII 1355 CCGG C^CGG
2363 CCGG
MseI 539 TTAA T^TAA
556 TTAA
MspI 1355 CCGG C^CGG
2363 CCGG
NdeI 2123 CATATG CA^TATG
2375 CATATG
RsaI 96 GTAC GT^AC
217 GTAC
SfcI 41 CTATAG C^T[GA][CT]AG
583 CTGCAG
TaqI 981 TCGA T^CGA
1089 TCGA
Tru9I 539 TTAA T^TAA
556 TTAA
Tsp45I 22 GTGAC ^GT[GC]AC
567 GTCAC
TthHB8I 981 TCGA T^CGA
1089 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
BbsI BpmI BpuAI BsaJI BsrI
BssKI BstF5I BstNI DdeI DpnII
EarI EcoRI EcoRII Fnu4HI FokI
HindIII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI ScrFI SfaNI Tsp509I TspRI
XmnI