Restriction map for
pF1KB9743
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BbeI BbrPI BbsI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp509I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 851 CGCG CG^CG
AgeI 761 ACCGGT A^CCGGT
AlwI 753 GATCC GGATC(4/5)
AlwNI 352 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 167 CCTCAGG CC^T[ACGT]AGG
AspI 559 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 929 TGCGCA TGC^GCA
BbvCI 240 CCTCAGC CCTCAGC(-5/-2)
BcgI 588 CGAGAAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1097 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 862 TACGTG [CT]AC^GT[GA]
BsaI 1290 GGTCTC GGTCTC(1/5)
BsaWI 761 ACCGGT [AT]^CCGG[AT]
BseRI 438 CTCCTC GAGGAG(10/8)
BsiEI 763 CGGTCG CG[GA][CT]^CG
BsmAI 1291 GTCTC GTCTC(1/5)
BsrBI 19 CCGCTC CCGCTC(-3/-3)
BstUI 851 CGCG CG^CG
BstYI 752 AGATCC [GA]^GATC[CT]
Bsu36I 167 CCTCAGG CC^T[ACGT]AGG
Eco81I 167 CCTCAGG CC^T[ACGT]AGG
FspI 929 TGCGCA TGC^GCA
MflI 752 AGATCC [GA]^GATC[CT]
MseI 842 TTAA T^TAA
MslI 769 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 851 CGCG CG^CG
PflFI 559 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 761 ACCGGT A^CCGGT
PpuMI 317 GGGACCT [GA]G^G[AT]CC[CT]
SexAI 551 ACCTGGT A^CC[AT]GGT
TaqI 23 TCGA T^CGA
Tru9I 842 TTAA T^TAA
Tth111I 559 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 23 TCGA T^CGA
XcmI 1355 CCACCAGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 752 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 1386 GACGCC G[GA]^CG[CT]C
1477 GGCGTC
AfaI 603 GTAC GT^AC
657 GTAC
Aor51HI 815 AGCGCT AGC^GCT
905 AGCGCT
BalI 951 TGGCCA TGG^CCA
1232 TGGCCA
BanI 1350 GGTGCC G^G[CT][GA]CC
1453 GGCACC
BsaBI 663 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1056 GATCATCATC
BsaHI 1386 GACGCC G[GA]^CG[CT]C
1477 GGCGTC
BsgI 106 CTGCAC GTGCAG(16/14)
424 CTGCAC
BsrFI 761 ACCGGT [GA]^CCGG[CT]
1557 ACCGGC
BssSI 967 CACGAG CACGAG(-5/-1)
232 CTCGTG
BstXI 494 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1358 CCAGGGCCCTGG
Cfr10I 761 ACCGGT [GA]^CCGG[CT]
1557 ACCGGC
DpnII 753 GATC ^GATC
1056 GATC
DraIII 859 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
955 CACTTTGTG
Eco47III 815 AGCGCT AGC^GCT
905 AGCGCT
Hin1I 1386 GACGCC G[GA]^CG[CT]C
1477 GGCGTC
HincII 565 GTCAAC GT[CT]^[GA]AC
1480 GTCAAC
HindII 565 GTCAAC GT[CT]^[GA]AC
1480 GTCAAC
HinfI 268 GACTC G^A[ACGT]TC
1436 GACTC
MaeII 788 ACGT A^CGT
863 ACGT
MamI 663 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1056 GATCATCATC
MboI 753 GATC ^GATC
1056 GATC
MboII 750 GAAGA GAAGA(8/7)
395 TCTTC
MluNI 951 TGGCCA TGG^CCA
1232 TGGCCA
MscI 951 TGGCCA TGG^CCA
1232 TGGCCA
NcoI 633 CCATGG C^CATGG
948 CCATGG
NdeII 753 GATC ^GATC
1056 GATC
PflMI 253 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
858 CCACTACGTGG
PleI 268 GACTC GAGTC(4/5)
1436 GACTC
PstI 717 CTGCAG CTGCA^G
985 CTGCAG
RsaI 603 GTAC GT^AC
657 GTAC
SacI 538 GAGCTC GAGCT^C
979 GAGCTC
Sau3AI 753 GATC ^GATC
1056 GATC
SfcI 717 CTGCAG C^T[GA][CT]AG
985 CTGCAG
Tsp45I 249 GTCAC ^GT[GC]AC
833 GTGAC
Van91I 253 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
858 CCACTACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI ApaI AspHI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeIII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PvuII
Sau96I ScrFI SfaNI StyI TseI
TspRI