Restriction map for
pF1KB9667
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AflIII AgeI AhdI ApaI
AscI AseI AsnI Asp718I AspEI
AvrII BalI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpuAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I EcoO109I EcoO65I EcoRV
EcoT22I FauI FbaI FseI HapII
Hin1I HincII HindII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmeI PpuMI PshAI PshBI
PvuI PvuII RcaI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SspBI
SspI SwaI TfiI Tsp45I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccII 132 CGCG CG^CG
AclI 818 AACGTT AA^CGTT
Alw44I 738 GTGCAC G^TGCAC
AlwNI 7 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 178 CCTCAGG CC^T[ACGT]AGG
Aor51HI 761 AGCGCT AGC^GCT
ApaLI 738 GTGCAC G^TGCAC
AspI 646 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 641 CCCGAG C^[CT]CG[GA]G
AvaII 637 GGTCC G^G[AT]CC
AviII 797 TGCGCA TGC^GCA
BbrPI 793 CACGTG CAC^GTG
BlpI 73 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 73 GCTAAGC GC^T[ACGT]AGC
BsaAI 793 CACGTG [CT]AC^GT[GA]
BsaI 644 GAGACC GGTCTC(1/5)
BseRI 625 CTCCTC GAGGAG(10/8)
BsiWI 787 CGTACG C^GTACG
BsmI 220 GAATGC GAATGC(1/-1)
BsoBI 641 CCCGAG C^[CT]CG[GA]G
BspMI 104 ACCTGC ACCTGC(4/8)
BstAPI 505 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 331 TTCGAA TT^CGAA
BstUI 132 CGCG CG^CG
BstYI 489 AGATCC [GA]^GATC[CT]
Bsu36I 178 CCTCAGG CC^T[ACGT]AGG
CelII 73 GCTAAGC GC^T[ACGT]AGC
Eco47III 761 AGCGCT AGC^GCT
Eco81I 178 CCTCAGG CC^T[ACGT]AGG
EcoNI 255 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 414 GAATTC G^AATTC
EcoT14I 281 CCTTGG C^C[AT][AT]GG
FspI 797 TGCGCA TGC^GCA
HaeII 761 AGCGCT [GA]GCGC^[CT]
HgaI 224 GCGTC GACGC(5/10)
HindIII 451 AAGCTT A^AGCTT
HphI 235 GGTGA GGTGA(8/7)
MaeIII 212 GTTAC ^GT[ACGT]AC
MflI 489 AGATCC [GA]^GATC[CT]
MseI 779 TTAA T^TAA
MslI 649 CACGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 132 CGCG CG^CG
NlaIV 637 GGTCCC GG[ACGT]^[ACGT]CC
NspI 894 GCATGC [GA]CATG^[CT]
NspV 331 TTCGAA TT^CGAA
PflFI 646 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 793 CACGTG CAC^GTG
PmlI 793 CACGTG CAC^GTG
Psp1406I 818 AACGTT AA^CGTT
PstI 106 CTGCAG CTGCA^G
SacI 684 GAGCTC GAGCT^C
SapI 56 GAAGAGC GCTCTTC(1/4)
SbfI 105 CCTGCAGG CCTGCA^GG
SfcI 106 CTGCAG C^T[GA][CT]AG
SfuI 331 TTCGAA TT^CGAA
SmlI 421 CTTGAG C^T[CT][GA]AG
SphI 894 GCATGC GCATG^C
Sse8387I 105 CCTGCAGG CCTGCA^GG
StuI 45 AGGCCT AGG^CCT
StyI 281 CCTTGG C^C[AT][AT]GG
TaqI 332 TCGA T^CGA
Tru9I 779 TTAA T^TAA
TspRI 66 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 646 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 332 TCGA T^CGA
XhoII 489 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 414 GAATTC [GA]^AATT[CT]
508 GAATTT
AfaI 435 GTAC GT^AC
788 GTAC
AlwI 490 GATCC GGATC(4/5)
301 GGATC
ApoI 414 GAATTC [GA]^AATT[CT]
508 GAATTT
Asp700I 669 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
816 GAAACGTTTC
AspHI 684 GAGCTC G[AT]GC[AT]^C
738 GTGCAC
BanII 59 GAGCCC G[GA]GC[CT]^C
684 GAGCTC
BbvI 607 GCTGC GCAGC(8/12)
842 GCTGC
BfaI 515 CTAG C^TAG
587 CTAG
BpmI 461 CTGGAG CTGGAG(16/14)
719 CTGGAG
BsiHKAI 684 GAGCTC G[AT]GC[AT]^C
738 GTGCAC
BsmAI 644 GAGAC GTCTC(1/5)
699 GAGAC
BsmFI 638 GTCCC GGGAC(10/14)
830 GTCCC
BsrI 718 ACTGG ACTGG(1/-1)
65 CCAGT
DpnII 302 GATC ^GATC
490 GATC
EarI 56 GAAGAG CTCTTC(1/4)
259 GAAGAG
Fnu4HI 607 GCTGC GC^[ACGT]GC
842 GCTGC
HinfI 265 GACTC G^A[ACGT]TC
277 GAGTC
ItaI 607 GCTGC GC^[ACGT]GC
842 GCTGC
Ksp632I 56 GAAGAG CTCTTC(1/4)
259 GAAGAG
MaeI 515 CTAG C^TAG
587 CTAG
MboI 302 GATC ^GATC
490 GATC
NdeII 302 GATC ^GATC
490 GATC
PleI 265 GACTC GAGTC(4/5)
277 GAGTC
RsaI 435 GTAC GT^AC
788 GTAC
Sau3AI 302 GATC ^GATC
490 GATC
TseI 607 GCTGC G^C[AT]GC
842 GCTGC
XmnI 669 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
816 GAAACGTTTC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
FokI HaeIII HhaI HinP1I MaeII
MboII MnlI MvaI MwoI NlaIII
Sau96I ScrFI SfaNI Tsp509I