Restriction map for
pF1KB9664
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwNI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaII AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaJI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Cac8I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI PacI PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AatII 278 GACGTC GACGT^C
AciI 1350 GCGG CCGC(-3/-1)
AcyI 278 GACGTC G[GA]^CG[CT]C
AgeI 1094 ACCGGT A^CCGGT
AocI 256 CCTCAGG CC^T[ACGT]AGG
Aor51HI 989 AGCGCT AGC^GCT
AvaI 741 CTCGAG C^[CT]CG[GA]G
BamHI 441 GGATCC G^GATCC
BbvI 1298 GCAGC GCAGC(8/12)
BfaI 73 CTAG C^TAG
BglI 413 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 278 GACGTC G[GA]^CG[CT]C
BsaI 680 GAGACC GGTCTC(1/5)
BsaWI 1094 ACCGGT [AT]^CCGG[AT]
BsmBI 280 CGTCTC CGTCTC(1/5)
BsmFI 131 GGGAC GGGAC(10/14)
BsoBI 741 CTCGAG C^[CT]CG[GA]G
BspMI 998 ACCTGC ACCTGC(4/8)
BsrDI 714 GCAATG GCAATG(2/0)
BsrFI 1094 ACCGGT [GA]^CCGG[CT]
BssKI 818 CCAGG ^CC[ACGT]GG
BstBI 163 TTCGAA TT^CGAA
BstNI 818 CCAGG CC^[AT]GG
Bsu36I 256 CCTCAGG CC^T[ACGT]AGG
CfoI 990 GCGC GCG^C
Cfr10I 1094 ACCGGT [GA]^CCGG[CT]
Cfr13I 1034 GGCCC G^G[ACGT]CC
DraII 1033 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 989 AGCGCT AGC^GCT
Eco81I 256 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1033 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 818 CCAGG ^CC[AT]GG
FauI 1350 GCGGG CCCGC(4/6)
Fnu4HI 1298 GCAGC GC^[ACGT]GC
HaeII 989 AGCGCT [GA]GCGC^[CT]
HapII 1095 CCGG C^CGG
HhaI 990 GCGC GCG^C
Hin1I 278 GACGTC G[GA]^CG[CT]C
HinP1I 990 GCGC G^CGC
HincII 89 GTCAAC GT[CT]^[GA]AC
HindII 89 GTCAAC GT[CT]^[GA]AC
HpaII 1095 CCGG C^CGG
ItaI 1298 GCAGC GC^[ACGT]GC
MaeI 73 CTAG C^TAG
MaeII 279 ACGT A^CGT
MspA1I 1348 CAGCGG C[AC]G^C[GT]G
MspI 1095 CCGG C^CGG
MvaI 818 CCAGG CC^[AT]GG
MwoI 413 GCCAGAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 587 CATATG CA^TATG
NspV 163 TTCGAA TT^CGAA
PaeR7I 741 CTCGAG C^TCGAG
PinAI 1094 ACCGGT A^CCGGT
PstI 605 CTGCAG CTGCA^G
Sau96I 1034 GGCCC G^G[ACGT]CC
ScrFI 818 CCAGG CC^[ACGT]GG
SfcI 605 CTGCAG C^T[GA][CT]AG
SfuI 163 TTCGAA TT^CGAA
SgrAI 1093 CACCGGTG C[GA]^CCGG[CT]G
TseI 1298 GCAGC G^C[AT]GC
Tsp509I 12 AATT ^AATT
XcmI 540 CCATAATAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 741 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BalI 2 TGGCCA TGG^CCA
420 TGGCCA
BsaBI 476 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
577 GATGGTCATC
EaeI 2 TGGCCA [CT]^GGCC[GA]
420 TGGCCA
EarI 146 CTCTTC CTCTTC(1/4)
228 CTCTTC
Ksp632I 146 CTCTTC CTCTTC(1/4)
228 CTCTTC
MamI 476 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
577 GATGGTCATC
MluNI 2 TGGCCA TGG^CCA
420 TGGCCA
MscI 2 TGGCCA TGG^CCA
420 TGGCCA
MslI 583 CATCCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
769 CACACAGATG
NlaIII 472 CATG CATG^
844 CATG
NlaIV 63 GGAGCC GG[ACGT]^[ACGT]CC
441 GGATCC
TfiI 39 GAATC G^A[AT]TC
433 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI AlwI Asp700I AspHI
BanII BmyI BpmI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BstF5I BstYI DdeI DpnII
FokI HaeIII HinfI HphI MaeIII
MboI MboII MflI MnlI MseI
NdeII PleI RsaI SacI Sau3AI
SfaNI SmlI TaqI Tru9I Tsp45I
TspRI TthHB8I XhoII XmnI