Restriction map for
pF1KB9663
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvaI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 131 GTAGAC GT^[AC][GT]AC
AcsI 392 GAATTC [GA]^AATT[CT]
ApoI 392 GAATTC [GA]^AATT[CT]
Asp700I 267 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 590 CCCGGG C^[CT]CG[GA]G
BamHI 308 GGATCC G^GATCC
BbsI 612 GAAGAC GAAGAC(2/6)
BpmI 401 CTGGAG CTGGAG(16/14)
BpuAI 612 GAAGAC GAAGAC(2/6)
BsaI 664 GGTCTC GGTCTC(1/5)
BsgI 504 GTGCAG GTGCAG(16/14)
BsmAI 665 GTCTC GTCTC(1/5)
BsoBI 590 CCCGGG C^[CT]CG[GA]G
BstF5I 150 GGATG GGATG(2/0)
Cac8I 80 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 69 GCGC GCG^C
EaeI 432 TGGCCG [CT]^GGCC[GA]
EarI 571 GAAGAG CTCTTC(1/4)
EcoRI 392 GAATTC G^AATTC
EcoT14I 45 CCAAGG C^C[AT][AT]GG
FauI 22 GCGGG CCCGC(4/6)
FokI 150 GGATG GGATG(9/13)
HhaI 69 GCGC GCG^C
HinP1I 69 GCGC G^CGC
HphI 609 GGTGA GGTGA(8/7)
Ksp632I 571 GAAGAG CTCTTC(1/4)
MspA1I 85 CAGCGG C[AC]G^C[GT]G
MwoI 71 GCAAAACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 80 GCCGGC GCC^GGC
NgoMIV 80 GCCGGC G^CCGGC
NlaIV 308 GGATCC GG[ACGT]^[ACGT]CC
SfaNI 300 GCATC GCATC(5/9)
SmaI 590 CCCGGG CCC^GGG
StuI 53 AGGCCT AGG^CCT
StyI 45 CCAAGG C^C[AT][AT]GG
Tsp509I 393 AATT ^AATT
XmaCI 590 CCCGGG C^CCGGG
XmaI 590 CCCGGG C^CCGGG
XmnI 267 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 22 GCGG CCGC(-3/-1)
87 GCGG
AfaI 170 GTAC GT^AC
641 GTAC
BbvI 84 GCAGC GCAGC(8/12)
549 GCAGC
BcnI 590 CCCGG CC^[GC]GG
591 CCGGG
BsrFI 80 GCCGGC [GA]^CCGG[CT]
434 GCCGGT
BssKI 590 CCCGG ^CC[ACGT]GG
591 CCGGG
BstYI 224 AGATCC [GA]^GATC[CT]
308 GGATCC
Cfr10I 80 GCCGGC [GA]^CCGG[CT]
434 GCCGGT
DdeI 136 CTCAG C^T[ACGT]AG
544 CTGAG
DpnII 225 GATC ^GATC
309 GATC
MboI 225 GATC ^GATC
309 GATC
MflI 224 AGATCC [GA]^GATC[CT]
308 GGATCC
MslI 247 CATAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
555 CATGAAGGTG
NciI 590 CCCGG CC^[GC]GG
591 CCGGG
NdeII 225 GATC ^GATC
309 GATC
NlaIII 102 CATG CATG^
555 CATG
RsaI 170 GTAC GT^AC
641 GTAC
Sau3AI 225 GATC ^GATC
309 GATC
ScrFI 590 CCCGG CC^[ACGT]GG
591 CCGGG
SmlI 39 CTCAAG C^T[CT][GA]AG
120 CTCAAG
TaqI 111 TCGA T^CGA
303 TCGA
TseI 84 GCAGC G^C[AT]GC
549 GCAGC
TthHB8I 111 TCGA T^CGA
303 TCGA
XhoII 224 AGATCC [GA]^GATC[CT]
308 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI AlwI AspHI BanII BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI Fnu4HI HaeIII HapII HindIII
HinfI HpaII ItaI MboII MnlI
MspI PleI SacI TspRI