Restriction map for
pF1KB9662
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrDI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I ClaI CpoI
DpnII DraI DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HpaI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI Sau3AI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 464 GGTACC G^GTACC
AcsI 634 AAATTC [GA]^AATT[CT]
AcyI 255 GGCGCC G[GA]^CG[CT]C
AflIII 482 ACACGT A^C[GA][CT]GT
AocI 459 CCTGAGG CC^T[ACGT]AGG
ApaI 522 GGGCCC GGGCC^C
ApoI 634 AAATTC [GA]^AATT[CT]
Asp718I 464 GGTACC G^GTACC
BanII 522 GGGCCC G[GA]GC[CT]^C
BbeI 255 GGCGCC GGCGC^C
BbsI 979 GAAGAC GAAGAC(2/6)
BglI 303 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 169 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 169 GCTCAGC GC^T[ACGT]AGC
BpuAI 979 GAAGAC GAAGAC(2/6)
BsaAI 530 TACGTG [CT]AC^GT[GA]
BsaHI 255 GGCGCC G[GA]^CG[CT]C
BsmAI 601 GAGAC GTCTC(1/5)
Bsp1407I 278 TGTACA T^GTACA
BsrBI 291 CCGCTC CCGCTC(-3/-3)
BsrFI 889 ACCGGC [GA]^CCGG[CT]
BsrGI 278 TGTACA T^GTACA
Bsu36I 459 CCTGAGG CC^T[ACGT]AGG
CelII 169 GCTCAGC GC^T[ACGT]AGC
Cfr10I 889 ACCGGC [GA]^CCGG[CT]
DraIII 804 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 29 CGGCCG [CT]^GGCC[GA]
EagI 29 CGGCCG C^GGCCG
EclXI 29 CGGCCG C^GGCCG
Eco52I 29 CGGCCG C^GGCCG
Eco81I 459 CCTGAGG CC^T[ACGT]AGG
EcoNI 13 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 255 GGCGCC G[GA]^CG[CT]C
HindIII 937 AAGCTT A^AGCTT
HinfI 446 GAATC G^A[ACGT]TC
HphI 803 TCACC GGTGA(8/7)
KasI 255 GGCGCC G^GCGCC
KpnI 464 GGTACC GGTAC^C
KspI 178 CCGCGG CCGC^GG
MseI 902 TTAA T^TAA
NarI 255 GGCGCC GG^CGCC
NdeI 771 CATATG CA^TATG
PflMI 726 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 178 CCGCGG CCGC^GG
SexAI 44 ACCAGGT A^CC[AT]GGT
SfoI 255 GGCGCC GGC^GCC
SmaI 197 CCCGGG CCC^GGG
SmlI 382 CTCAAG C^T[CT][GA]AG
SspBI 278 TGTACA T^GTACA
TfiI 446 GAATC G^A[AT]TC
Tru9I 902 TTAA T^TAA
Van91I 726 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 911 CCAAAGAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 197 CCCGGG C^CCGGG
XmaI 197 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 279 GTAC GT^AC
465 GTAC
AvaII 284 GGTCC G^G[AT]CC
399 GGTCC
BpmI 17 CTCCAG CTGGAG(16/14)
841 CTGGAG
BsgI 387 GTGCAG GTGCAG(16/14)
810 GTGCAG
BsiEI 29 CGGCCG CG[GA][CT]^CG
645 CGACCG
BsrI 604 ACTGG ACTGG(1/-1)
796 CCAGT
BssHII 549 GCGCGC G^CGCGC
836 GCGCGC
BstF5I 378 CATCC GGATG(2/0)
509 GGATG
FokI 378 CATCC GGATG(9/13)
509 GGATG
MaeIII 802 GTCAC ^GT[ACGT]AC
878 GTGAC
MslI 483 CACGTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
758 CATTCAGGTG
NcoI 488 CCATGG C^CATGG
1004 CCATGG
PstI 89 CTGCAG CTGCA^G
947 CTGCAG
PvuII 210 CAGCTG CAG^CTG
627 CAGCTG
RsaI 279 GTAC GT^AC
465 GTAC
SfaNI 543 GCATC GCATC(5/9)
896 GCATC
SfcI 89 CTGCAG C^T[GA][CT]AG
947 CTGCAG
StuI 93 AGGCCT AGG^CCT
782 AGGCCT
TaqI 269 TCGA T^CGA
341 TCGA
Tsp45I 802 GTCAC ^GT[GC]AC
878 GTGAC
Tsp509I 635 AATT ^AATT
999 AATT
TthHB8I 269 TCGA T^CGA
341 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
BanI BbvI BcnI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MaeII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI StyI TseI
TspRI