Restriction map for
pF1KB9661
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcsI 652 AAATTC [GA]^AATT[CT]
AcyI 913 GGCGTC G[GA]^CG[CT]C
ApaI 540 GGGCCC GGGCC^C
ApoI 652 AAATTC [GA]^AATT[CT]
AvaII 328 GGACC G^G[AT]CC
BbvCI 429 GCTGAGG CCTCAGC(-5/-2)
BciVI 498 GGATAC GTATCC(6/5)
BfaI 949 CTAG C^TAG
BglII 452 AGATCT A^GATCT
BpmI 17 CTCCAG CTGGAG(16/14)
BsaAI 566 TACGTG [CT]AC^GT[GA]
BsaHI 913 GGCGTC G[GA]^CG[CT]C
BsaWI 165 ACCGGA [AT]^CCGG[AT]
BseRI 424 GAGGAG GAGGAG(10/8)
BsiEI 163 CGACCG CG[GA][CT]^CG
BsmFI 327 GGGAC GGGAC(10/14)
BsrDI 512 GCAATG GCAATG(2/0)
BstEII 180 GGTGACC G^GT[ACGT]ACC
BstPI 180 GGTGACC G^GT[ACGT]ACC
DdeI 430 CTGAG C^T[ACGT]AG
DraIII 822 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 323 CCGTGG C^C[GA][CT]GG
EcoO65I 180 GGTGACC G^GT[ACGT]ACC
EcoT14I 494 CCAAGG C^C[AT][AT]GG
Hin1I 913 GGCGTC G[GA]^CG[CT]C
MaeI 949 CTAG C^TAG
PleI 282 GAGTC GAGTC(4/5)
PshAI 348 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 859 CTGCAG CTGCA^G
SmlI 246 CTTGAG C^T[CT][GA]AG
StyI 494 CCAAGG C^C[AT][AT]GG
TfiI 464 GAATC G^A[AT]TC
XcmI 900 CCAGCAGACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 382 CGCG CG^CG
881 CGCG
Alw44I 42 GTGCAC G^TGCAC
854 GTGCAC
AlwI 417 GATCC GGATC(4/5)
33 GGATC
ApaLI 42 GTGCAC G^TGCAC
854 GTGCAC
AspHI 42 GTGCAC G[AT]GC[AT]^C
854 GTGCAC
AvaI 277 CCCGAG C^[CT]CG[GA]G
556 CCCGAG
BcgI 846 CGAGAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1005 CGATGAGGATGC
BsgI 150 CTGCAC GTGCAG(16/14)
828 GTGCAG
BsiHKAI 42 GTGCAC G[AT]GC[AT]^C
854 GTGCAC
BsoBI 277 CCCGAG C^[CT]CG[GA]G
556 CCCGAG
BsrI 622 ACTGG ACTGG(1/-1)
814 CCAGT
BstUI 382 CGCG CG^CG
881 CGCG
BstYI 416 AGATCC [GA]^GATC[CT]
452 AGATCT
Cac8I 103 GCCTGC GC[ACGT]^[ACGT]GC
830 GCAGGC
DraII 539 AGGGCCC [GA]G^G[ACGT]CC[CT]
540 GGGCCCC
EcoO109I 539 AGGGCCC [GA]G^G[ACGT]CC[CT]
540 GGGCCCC
FauI 879 CCCGC CCCGC(4/6)
177 GCGGG
HaeII 95 AGCGCC [GA]GCGC^[CT]
552 AGCGCC
HaeIII 102 GGCC GG^CC
541 GGCC
HgaI 86 GACGC GACGC(5/10)
914 GCGTC
HinfI 282 GAGTC G^A[ACGT]TC
464 GAATC
MflI 416 AGATCC [GA]^GATC[CT]
452 AGATCT
MslI 562 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
776 CATTCAAGTG
MspA1I 350 CAGCTG C[AC]G^C[GT]G
645 CAGCTG
MvnI 382 CGCG CG^CG
881 CGCG
PvuII 350 CAGCTG CAG^CTG
645 CAGCTG
SfcI 859 CTGCAG C^T[GA][CT]AG
965 CTACAG
XhoII 416 AGATCC [GA]^GATC[CT]
452 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI BanII BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI CfoI Cfr13I DpnII EarI
EcoRII Fnu4HI FokI HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I