Restriction map for
pF1KB9658
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HinfI
HpaI KpnI Ksp632I MaeIII MamI
MfeI MroI MunI NdeI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I Tsp509I Tth111I
Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AflII 838 CTTAAG C^TTAAG
Aor51HI 327 AGCGCT AGC^GCT
BalI 47 TGGCCA TGG^CCA
BanI 439 GGCGCC G^G[CT][GA]CC
BbeI 439 GGCGCC GGCGC^C
BfrI 838 CTTAAG C^TTAAG
BpmI 796 CTGGAG CTGGAG(16/14)
BseRI 948 CTCCTC GAGGAG(10/8)
BsgI 135 CTGCAC GTGCAG(16/14)
BsiEI 224 CGACCG CG[GA][CT]^CG
BsmAI 872 GAGAC GTCTC(1/5)
BsmBI 872 GAGACG CGTCTC(1/5)
BsmFI 852 GTCCC GGGAC(10/14)
BsrBI 175 CCGCTC CCGCTC(-3/-3)
BssHII 111 GCGCGC G^CGCGC
BssSI 646 CTCGTG CACGAG(-5/-1)
BstF5I 260 GGATG GGATG(2/0)
BstNI 795 CCTGG CC^[AT]GG
BstXI 38 CCAGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 249 GGATCT [GA]^GATC[CT]
DraII 130 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 327 AGCGCT AGC^GCT
EcoO109I 130 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 795 CCTGG ^CC[AT]GG
FokI 260 GGATG GGATG(9/13)
HincII 61 GTCAAC GT[CT]^[GA]AC
HindII 61 GTCAAC GT[CT]^[GA]AC
HindIII 366 AAGCTT A^AGCTT
KasI 439 GGCGCC G^GCGCC
KspI 5 CCGCGG CCGC^GG
MaeII 89 ACGT A^CGT
MflI 249 GGATCT [GA]^GATC[CT]
MluNI 47 TGGCCA TGG^CCA
MscI 47 TGGCCA TGG^CCA
MseI 839 TTAA T^TAA
MslI 654 CATGTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 795 CCTGG CC^[AT]GG
NarI 439 GGCGCC GG^CGCC
NcoI 50 CCATGG C^CATGG
NheI 529 GCTAGC G^CTAGC
PaeR7I 244 CTCGAG C^TCGAG
PstI 145 CTGCAG CTGCA^G
PvuII 544 CAGCTG CAG^CTG
SacII 5 CCGCGG CCGC^GG
SfcI 145 CTGCAG C^T[GA][CT]AG
SfoI 439 GGCGCC GGC^GCC
SgrAI 441 CGCCGGCG C[GA]^CCGG[CT]G
Tru9I 839 TTAA T^TAA
XcmI 35 CCACCAGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 244 CTCGAG C^TCGAG
XhoII 249 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 255 GTAC GT^AC
432 GTAC
AflIII 428 ACGCGT A^C[GA][CT]GT
774 ACGCGT
AlwI 708 GATCC GGATC(4/5)
249 GGATC
AlwNI 129 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
355 CAGCCGCTG
ApaI 465 GGGCCC GGGCC^C
565 GGGCCC
AvaII 624 GGACC G^G[AT]CC
731 GGACC
BbsI 234 GAAGAC GAAGAC(2/6)
807 GAAGAC
BfaI 530 CTAG C^TAG
554 CTAG
BglI 102 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
442 GCCGGCGTGGC
BlpI 12 GCTGAGC GC^T[ACGT]AGC
177 GCTCAGC
Bpu1102I 12 GCTGAGC GC^T[ACGT]AGC
177 GCTCAGC
BpuAI 234 GAAGAC GAAGAC(2/6)
807 GAAGAC
BsrFI 154 GCCGGC [GA]^CCGG[CT]
442 GCCGGC
CelII 12 GCTGAGC GC^T[ACGT]AGC
177 GCTCAGC
Cfr10I 154 GCCGGC [GA]^CCGG[CT]
442 GCCGGC
DdeI 13 CTGAG C^T[ACGT]AG
178 CTCAG
DpnII 250 GATC ^GATC
708 GATC
DraIII 183 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
343 CACCCAGTG
DsaI 5 CCGCGG C^C[GA][CT]GG
50 CCATGG
EaeI 2 TGGCCG [CT]^GGCC[GA]
47 TGGCCA
EcoT14I 50 CCATGG C^C[AT][AT]GG
695 CCAAGG
MaeI 530 CTAG C^TAG
554 CTAG
MboI 250 GATC ^GATC
708 GATC
MluI 428 ACGCGT A^CGCGT
774 ACGCGT
NaeI 154 GCCGGC GCC^GGC
442 GCCGGC
NdeII 250 GATC ^GATC
708 GATC
NgoMIV 154 GCCGGC G^CCGGC
442 GCCGGC
RsaI 255 GTAC GT^AC
432 GTAC
Sau3AI 250 GATC ^GATC
708 GATC
StyI 50 CCATGG C^C[AT][AT]GG
695 CCAAGG
TspRI 104 CACTG CA[GC]TG[ACGT][ACGT]^
347 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BanII BbvI BcnI BmyI BsaHI
BsaJI BsiYI BslI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstUI
Cac8I CfoI Cfr13I FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MboII MnlI MspA1I MspI MvnI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SmaI SmlI TaqI TseI
TthHB8I XmaCI XmaI