Restriction map for
pF1KB9656
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DpnII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HgaI HincII HindII HinfI HpaI
HphI KpnI KspI MaeII MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI NaeI NciI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 42 CGCG CG^CG
AcsI 360 AAATTT [GA]^AATT[CT]
AcyI 242 GGCGCC G[GA]^CG[CT]C
AocI 148 CCTGAGG CC^T[ACGT]AGG
ApoI 360 AAATTT [GA]^AATT[CT]
AvaI 100 CCCGAG C^[CT]CG[GA]G
AvaII 228 GGACC G^G[AT]CC
BanI 242 GGCGCC G^G[CT][GA]CC
BbeI 242 GGCGCC GGCGC^C
BbsI 111 GTCTTC GAAGAC(2/6)
BfaI 400 CTAG C^TAG
BpuAI 111 GTCTTC GAAGAC(2/6)
BsaHI 242 GGCGCC G[GA]^CG[CT]C
BsaWI 69 TCCGGT [AT]^CCGG[AT]
BsoBI 100 CCCGAG C^[CT]CG[GA]G
BsrDI 368 GCAATG GCAATG(2/0)
BsrI 443 ACTGG ACTGG(1/-1)
BssHII 41 GCGCGC G^CGCGC
BssKI 221 CCAGG ^CC[ACGT]GG
BstNI 221 CCAGG CC^[AT]GG
BstUI 42 CGCG CG^CG
Bsu36I 148 CCTGAGG CC^T[ACGT]AGG
Cfr13I 228 GGACC G^G[ACGT]CC
DraI 304 TTTAAA TTT^AAA
DraII 227 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 251 CCATGG C^C[GA][CT]GG
Eco81I 148 CCTGAGG CC^T[ACGT]AGG
EcoO109I 227 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 221 CCAGG ^CC[AT]GG
EcoT14I 251 CCATGG C^C[AT][AT]GG
FauI 330 CCCGC CCCGC(4/6)
HaeIII 65 GGCC GG^CC
HapII 70 CCGG C^CGG
Hin1I 242 GGCGCC G[GA]^CG[CT]C
HpaII 70 CCGG C^CGG
KasI 242 GGCGCC G^GCGCC
MaeI 400 CTAG C^TAG
MspI 70 CCGG C^CGG
MvaI 221 CCAGG CC^[AT]GG
MvnI 42 CGCG CG^CG
NarI 242 GGCGCC GG^CGCC
NcoI 251 CCATGG C^CATGG
PpuMI 227 AGGACCT [GA]G^G[AT]CC[CT]
SapI 28 GAAGAGC GCTCTTC(1/4)
Sau96I 228 GGACC G^G[ACGT]CC
ScrFI 221 CCAGG CC^[ACGT]GG
SfaNI 180 GATGC GCATC(5/9)
SfoI 242 GGCGCC GGC^GCC
StuI 64 AGGCCT AGG^CCT
StyI 251 CCATGG C^C[AT][AT]GG
TspRI 442 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BanII 154 GAGCTC G[GA]GC[CT]^C
166 GAGCTC
BbvI 258 GCAGC GCAGC(8/12)
388 GCAGC
BsmFI 344 GGGAC GGGAC(10/14)
96 GTCCC
Cac8I 41 GCGCGC GC[ACGT]^[ACGT]GC
329 GCCCGC
EarI 28 GAAGAG CTCTTC(1/4)
403 GAAGAG
EcoNI 186 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
231 CCTGTTTGAGG
HaeII 197 AGCGCC [GA]GCGC^[CT]
242 GGCGCC
HindIII 286 AAGCTT A^AGCTT
300 AAGCTT
Ksp632I 28 GAAGAG CTCTTC(1/4)
403 GAAGAG
MseI 305 TTAA T^TAA
364 TTAA
NlaIII 252 CATG CATG^
352 CATG
NlaIV 242 GGCGCC GG[ACGT]^[ACGT]CC
458 GGAGCC
SacI 154 GAGCTC GAGCT^C
166 GAGCTC
Tru9I 305 TTAA T^TAA
364 TTAA
TseI 258 GCAGC G^C[AT]GC
388 GCAGC
Tsp509I 335 AATT ^AATT
361 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AluI AspHI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
CfoI DdeI Fnu4HI HhaI HinP1I
ItaI MboII MnlI MwoI