Restriction map for
pF1KB9640
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BlnI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 561 GACGCC G[GA]^CG[CT]C
AlwNI 478 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 192 GGGCCC GGGCC^C
AspHI 493 GTGCTC G[AT]GC[AT]^C
BanI 354 GGCACC G^G[CT][GA]CC
BfaI 49 CTAG C^TAG
BglII 920 AGATCT A^GATCT
BlpI 717 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 717 GCTGAGC GC^T[ACGT]AGC
BsaAI 298 CACGTA [CT]AC^GT[GA]
BsaHI 561 GACGCC G[GA]^CG[CT]C
BsiHKAI 493 GTGCTC G[AT]GC[AT]^C
BsmI 29 GCATTC GAATGC(1/-1)
BstAPI 486 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 171 GGTGACC G^GT[ACGT]ACC
BstPI 171 GGTGACC G^GT[ACGT]ACC
BstXI 218 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 920 AGATCT [GA]^GATC[CT]
CelII 717 GCTGAGC GC^T[ACGT]AGC
DraII 191 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 196 CCATGG C^C[GA][CT]GG
EcoO109I 191 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 171 GGTGACC G^GT[ACGT]ACC
EcoT14I 196 CCATGG C^C[AT][AT]GG
FauI 67 GCGGG CCCGC(4/6)
HaeII 651 GGCGCT [GA]GCGC^[CT]
Hin1I 561 GACGCC G[GA]^CG[CT]C
MaeI 49 CTAG C^TAG
MflI 920 AGATCT [GA]^GATC[CT]
MslI 852 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 196 CCATGG C^CATGG
PflMI 332 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 696 CTGCAG CTGCA^G
SmaI 247 CCCGGG CCC^GGG
SmlI 892 CTCAAG C^T[CT][GA]AG
StuI 707 AGGCCT AGG^CCT
StyI 196 CCATGG C^C[AT][AT]GG
Tsp45I 172 GTGAC ^GT[GC]AC
Tsp509I 599 AATT ^AATT
Van91I 332 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 215 CCACCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 920 AGATCT [GA]^GATC[CT]
XmaCI 247 CCCGGG C^CCGGG
XmaI 247 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 253 CGCG CG^CG
912 CGCG
AfaI 399 GTAC GT^AC
874 GTAC
AvaI 157 CTCGGG C^[CT]CG[GA]G
247 CCCGGG
BalI 2 TGGCCA TGG^CCA
212 TGGCCA
BbsI 256 GAAGAC GAAGAC(2/6)
986 GAAGAC
BglI 337 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
346 GCCAGCCCGGC
BpuAI 256 GAAGAC GAAGAC(2/6)
986 GAAGAC
BsmAI 559 GAGAC GTCTC(1/5)
880 GAGAC
BsmBI 559 GAGACG CGTCTC(1/5)
880 GAGACG
BsmFI 444 GGGAC GGGAC(10/14)
899 GTCCC
BsoBI 157 CTCGGG C^[CT]CG[GA]G
247 CCCGGG
BspMI 260 ACCTGC ACCTGC(4/8)
698 GCAGGT
BsrBI 288 CCGCTC CCGCTC(-3/-3)
423 GAGCGG
BsrDI 535 CATTGC GCAATG(2/0)
143 GCAATG
BstF5I 151 CATCC GGATG(2/0)
279 CATCC
BstUI 253 CGCG CG^CG
912 CGCG
FokI 151 CATCC GGATG(9/13)
279 CATCC
HgaI 561 GACGC GACGC(5/10)
882 GACGC
MaeIII 172 GTGAC ^GT[ACGT]AC
671 GTAAC
MluNI 2 TGGCCA TGG^CCA
212 TGGCCA
MscI 2 TGGCCA TGG^CCA
212 TGGCCA
MvnI 253 CGCG CG^CG
912 CGCG
NspI 71 GCATGC [GA]CATG^[CT]
428 GCATGC
RsaI 399 GTAC GT^AC
874 GTAC
SfaNI 150 GCATC GCATC(5/9)
162 GCATC
SfcI 24 CTACAG C^T[GA][CT]AG
696 CTGCAG
SphI 71 GCATGC GCATG^C
428 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AflIII AluI AlwI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraIII
EaeI EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I ScrFI TaqI TfiI
TseI TspRI TthHB8I