Restriction map for
pF1KB9637
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp509I Tth111I TthHB8I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 359 GACGCC G[GA]^CG[CT]C
AlwI 432 GGATC GGATC(4/5)
Asp700I 48 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 167 GGCACC G^G[CT][GA]CC
BbvCI 616 GCTGAGG CCTCAGC(-5/-2)
BciVI 709 GTATCC GTATCC(6/5)
BsaHI 359 GACGCC G[GA]^CG[CT]C
BsaI 500 GAGACC GGTCTC(1/5)
BsmAI 500 GAGAC GTCTC(1/5)
BsmI 1225 GCATTC GAATGC(1/-1)
BstAPI 669 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 426 GCGC GCG^C
DraIII 364 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1127 CCATGG C^C[GA][CT]GG
EaeI 185 CGGCCA [CT]^GGCC[GA]
EarI 274 GAAGAG CTCTTC(1/4)
EcoNI 924 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1223 ATGCAT ATGCA^T
HgaI 359 GACGC GACGC(5/10)
HhaI 426 GCGC GCG^C
Hin1I 359 GACGCC G[GA]^CG[CT]C
HinP1I 426 GCGC G^CGC
Ksp632I 274 GAAGAG CTCTTC(1/4)
MaeII 52 ACGT A^CGT
MaeIII 864 GTCAC ^GT[ACGT]AC
MseI 1087 TTAA T^TAA
NcoI 1127 CCATGG C^CATGG
NsiI 1223 ATGCAT ATGCA^T
PflMI 363 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 682 GAGTC GAGTC(4/5)
PpuMI 197 GGGACCT [GA]G^G[AT]CC[CT]
PstI 941 CTGCAG CTGCA^G
SexAI 365 ACCTGGT A^CC[AT]GGT
SfcI 941 CTGCAG C^T[GA][CT]AG
TfiI 1069 GAATC G^A[AT]TC
Tru9I 1087 TTAA T^TAA
Tsp45I 864 GTCAC ^GT[GC]AC
Van91I 363 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1026 CCAAAATACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 48 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 141 GTAC GT^AC
750 GTAC
BbsI 48 GAAGAC GAAGAC(2/6)
456 GAAGAC
BcnI 344 CCCGG CC^[GC]GG
443 CCCGG
BfaI 229 CTAG C^TAG
287 CTAG
BpmI 85 CTCCAG CTGGAG(16/14)
223 CTGGAG
BpuAI 48 GAAGAC GAAGAC(2/6)
456 GAAGAC
BsaWI 59 ACCGGA [AT]^CCGG[AT]
496 ACCGGA
BseRI 722 CTCCTC GAGGAG(10/8)
124 GAGGAG
BspMI 923 ACCTGC ACCTGC(4/8)
966 ACCTGC
BstXI 101 CCAACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
414 CCAGAGATTTGG
DpnII 433 GATC ^GATC
892 GATC
DraII 197 GGGACCT [GA]G^G[ACGT]CC[CT]
1059 GGGGCCT
EcoO109I 197 GGGACCT [GA]G^G[ACGT]CC[CT]
1059 GGGGCCT
HinfI 682 GAGTC G^A[ACGT]TC
1069 GAATC
MaeI 229 CTAG C^TAG
287 CTAG
MboI 433 GATC ^GATC
892 GATC
MslI 28 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
237 CACCGAGGTG
NciI 344 CCCGG CC^[GC]GG
443 CCCGG
NdeII 433 GATC ^GATC
892 GATC
RsaI 141 GTAC GT^AC
750 GTAC
Sau3AI 433 GATC ^GATC
892 GATC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BbvI BmyI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MboII
MnlI MspA1I MspI MvaI MwoI
NlaIII NlaIV PvuII Sau96I ScrFI
SfaNI StyI TseI TspRI