Restriction map for
pF1KB9636
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AlwI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoNI EcoO65I EcoRV
EcoT22I FbaI HincII HindII HindIII
HpaI KpnI MaeII MamI MfeI
MluI MluNI MscI MseI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccIII 42 TCCGGA T^CCGGA
AcsI 3 GAATTC [GA]^AATT[CT]
AocI 63 CCTGAGG CC^T[ACGT]AGG
ApoI 3 GAATTC [GA]^AATT[CT]
AvaI 154 CCCGGG C^[CT]CG[GA]G
AviII 353 TGCGCA TGC^GCA
BanII 15 GAGCTC G[GA]GC[CT]^C
BfaI 301 CTAG C^TAG
BglI 772 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 449 AGATCT A^GATCT
BsaI 117 GGTCTC GGTCTC(1/5)
BsaWI 42 TCCGGA [AT]^CCGG[AT]
BseAI 42 TCCGGA T^CCGGA
BseRI 424 GAGGAG GAGGAG(10/8)
BsgI 720 CTGCAC GTGCAG(16/14)
BsmBI 106 CGTCTC CGTCTC(1/5)
BsoBI 154 CCCGGG C^[CT]CG[GA]G
BspEI 42 TCCGGA T^CCGGA
BsrBI 758 CCGCTC CCGCTC(-3/-3)
BsrFI 502 GCCGGC [GA]^CCGG[CT]
BsrI 698 ACTGG ACTGG(1/-1)
BssHII 560 GCGCGC G^CGCGC
BstXI 413 CCAGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 449 AGATCT [GA]^GATC[CT]
Bsu36I 63 CCTGAGG CC^T[ACGT]AGG
Cfr10I 502 GCCGGC [GA]^CCGG[CT]
DdeI 64 CTGAG C^T[ACGT]AG
DraII 236 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 535 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 432 GAAGAG CTCTTC(1/4)
Eco81I 63 CCTGAGG CC^T[ACGT]AGG
EcoO109I 236 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 3 GAATTC G^AATTC
FseI 501 GGCCGGCC GGCCGG^CC
FspI 353 TGCGCA TGC^GCA
HgaI 269 GACGC GACGC(5/10)
Ksp632I 432 GAAGAG CTCTTC(1/4)
KspI 188 CCGCGG CCGC^GG
MaeI 301 CTAG C^TAG
MaeIII 585 GTCAC ^GT[ACGT]AC
MflI 449 AGATCT [GA]^GATC[CT]
MroI 42 TCCGGA T^CCGGA
MslI 399 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 502 GCCGGC GCC^GGC
NcoI 305 CCATGG C^CATGG
NgoMIV 502 GCCGGC G^CCGGC
PleI 796 GAGTC GAGTC(4/5)
PpuMI 236 AGGACCT [GA]G^G[AT]CC[CT]
PstI 673 CTGCAG CTGCA^G
SacI 15 GAGCTC GAGCT^C
SacII 188 CCGCGG CCGC^GG
SmaI 154 CCCGGG CCC^GGG
TaqI 13 TCGA T^CGA
TfiI 10 GATTC G^A[AT]TC
Tsp45I 585 GTCAC ^GT[GC]AC
Tsp509I 4 AATT ^AATT
TthHB8I 13 TCGA T^CGA
XhoII 449 AGATCT [GA]^GATC[CT]
XmaCI 154 CCCGGG C^CCGGG
XmaI 154 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 178 GGCGCC G[GA]^CG[CT]C
651 GGCGCC
AfaI 60 GTAC GT^AC
207 GTAC
Alw44I 532 GTGCAC G^TGCAC
541 GTGCAC
ApaLI 532 GTGCAC G^TGCAC
541 GTGCAC
BbeI 178 GGCGCC GGCGC^C
651 GGCGCC
BpmI 24 CTCCAG CTGGAG(16/14)
421 CTGGAG
BsaHI 178 GGCGCC G[GA]^CG[CT]C
651 GGCGCC
BsiEI 500 CGGCCG CG[GA][CT]^CG
791 CGGCCG
BsmFI 68 GGGAC GGGAC(10/14)
484 GGGAC
BstNI 96 CCAGG CC^[AT]GG
420 CCTGG
EagI 500 CGGCCG C^GGCCG
791 CGGCCG
EclXI 500 CGGCCG C^GGCCG
791 CGGCCG
Eco52I 500 CGGCCG C^GGCCG
791 CGGCCG
EcoRII 96 CCAGG ^CC[AT]GG
420 CCTGG
HaeII 178 GGCGCC [GA]GCGC^[CT]
651 GGCGCC
Hin1I 178 GGCGCC G[GA]^CG[CT]C
651 GGCGCC
HinfI 10 GATTC G^A[ACGT]TC
796 GAGTC
HphI 410 TCACC GGTGA(8/7)
586 TCACC
KasI 178 GGCGCC G^GCGCC
651 GGCGCC
MvaI 96 CCAGG CC^[AT]GG
420 CCTGG
NarI 178 GGCGCC GG^CGCC
651 GGCGCC
PvuII 223 CAGCTG CAG^CTG
676 CAGCTG
RsaI 60 GTAC GT^AC
207 GTAC
SfcI 241 CTACAG C^T[GA][CT]AG
673 CTGCAG
SfoI 178 GGCGCC GGC^GCC
651 GGCGCC
SmlI 646 CTCAAG C^T[CT][GA]AG
811 CTCAAG
TspRI 478 CACTG CA[GC]TG[ACGT][ACGT]^
697 CACTG
XcmI 693 CCAGCACTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
767 CCACAGCCAACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanI BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BssKI BstAPI BstF5I BstUI Cac8I
CfoI Cfr13I DpnII DsaI EaeI
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboI MboII MnlI MspA1I MspI
MvnI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TseI