Restriction map for
pF1KB9632
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI NcoI NdeI
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp509I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AfaI 438 GTAC GT^AC
AhdI 502 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 167 GGGCCC GGGCC^C
AspEI 502 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 242 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 456 GGCGCC G^G[CT][GA]CC
BbeI 456 GGCGCC GGCGC^C
BbsI 76 GAAGAC GAAGAC(2/6)
BpuAI 76 GAAGAC GAAGAC(2/6)
BseRI 565 GAGGAG GAGGAG(10/8)
BsiEI 267 CGACCG CG[GA][CT]^CG
BsmI 284 GAATGC GAATGC(1/-1)
BspMI 88 ACCTGC ACCTGC(4/8)
BsrBI 52 GAGCGG CCGCTC(-3/-3)
DpnII 363 GATC ^GATC
DrdI 48 GACGGAGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 502 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HphI 356 TCACC GGTGA(8/7)
KasI 456 GGCGCC G^GCGCC
MaeII 97 ACGT A^CGT
MboI 363 GATC ^GATC
NarI 456 GGCGCC GG^CGCC
NdeII 363 GATC ^GATC
PflFI 242 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 508 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 438 GTAC GT^AC
Sau3AI 363 GATC ^GATC
SfoI 456 GGCGCC GGC^GCC
SgrAI 452 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 606 CTTGAG C^T[CT][GA]AG
Tth111I 242 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AviII 136 TGCGCA TGC^GCA
416 TGCGCA
BanII 167 GGGCCC G[GA]GC[CT]^C
609 GAGCCC
BpmI 445 CTGGAG CTGGAG(16/14)
562 CTGGAG
BsmBI 46 GAGACG CGTCTC(1/5)
367 GAGACG
BsrFI 206 GCCGGC [GA]^CCGG[CT]
453 GCCGGC
Cfr10I 206 GCCGGC [GA]^CCGG[CT]
453 GCCGGC
EarI 594 CTCTTC CTCTTC(1/4)
148 GAAGAG
FspI 136 TGCGCA TGC^GCA
416 TGCGCA
HaeII 399 GGCGCT [GA]GCGC^[CT]
456 GGCGCC
Ksp632I 594 CTCTTC CTCTTC(1/4)
148 GAAGAG
MaeIII 37 GTGAC ^GT[ACGT]AC
85 GTGAC
NaeI 206 GCCGGC GCC^GGC
453 GCCGGC
NgoMIV 206 GCCGGC G^CCGGC
453 GCCGGC
SmaI 496 CCCGGG CCC^GGG
574 CCCGGG
TaqI 282 TCGA T^CGA
365 TCGA
Tsp45I 37 GTGAC ^GT[GC]AC
85 GTGAC
TthHB8I 282 TCGA T^CGA
365 TCGA
XmaCI 496 CCCGGG C^CCGGG
574 CCCGGG
XmaI 496 CCCGGG C^CCGGG
574 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BbvI BcnI BmyI BsaHI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI MboII
MnlI MspI MvaI MvnI MwoI
NciI NlaIV PleI Sau96I ScrFI
TseI TspRI