Restriction map for
pF1KB9630
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspHI AspI AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EarI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI NcoI NdeI NheI
NotI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuII RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AhdI 544 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 380 AGCT AG^CT
AlwI 453 GGATC GGATC(4/5)
AscI 151 GGCGCGCC GG^CGCGCC
AspEI 544 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 218 GGCGCC GGCGC^C
BsaI 16 GAGACC GGTCTC(1/5)
BsgI 256 CTGCAC GTGCAG(16/14)
BsmBI 394 GAGACG CGTCTC(1/5)
BspMI 19 ACCTGC ACCTGC(4/8)
BsrI 596 CCAGT ACTGG(1/-1)
BssHII 152 GCGCGC G^CGCGC
BstAPI 258 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 222 CCGCGG C^C[GA][CT]GG
EagI 225 CGGCCG C^GGCCG
Eam1105I 544 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 225 CGGCCG C^GGCCG
Eco52I 225 CGGCCG C^GGCCG
KasI 218 GGCGCC G^GCGCC
KspI 222 CCGCGG CCGC^GG
MaeIII 59 GTGAC ^GT[ACGT]AC
NarI 218 GGCGCC GG^CGCC
NlaIII 312 CATG CATG^
NruI 297 TCGCGA TCG^CGA
NspI 311 GCATGC [GA]CATG^[CT]
PshAI 231 GACGCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 294 CGATCG CGAT^CG
SacII 222 CCGCGG CCGC^GG
SfaNI 23 GCATC GCATC(5/9)
SfoI 218 GGCGCC GGC^GCC
SmlI 13 CTTGAG C^T[CT][GA]AG
SphI 311 GCATGC GCATG^C
Tsp45I 59 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 171 GGCGTC G[GA]^CG[CT]C
218 GGCGCC
AvaI 167 CCCGGG C^[CT]CG[GA]G
479 CCCGGG
AvaII 237 GGTCC G^G[AT]CC
523 GGTCC
AviII 266 TGCGCA TGC^GCA
344 TGCGCA
BanII 425 GGGCTC G[GA]GC[CT]^C
558 GGGCTC
BbsI 88 GAAGAC GAAGAC(2/6)
616 GAAGAC
BpuAI 88 GAAGAC GAAGAC(2/6)
616 GAAGAC
BsaHI 171 GGCGTC G[GA]^CG[CT]C
218 GGCGCC
BsiEI 225 CGGCCG CG[GA][CT]^CG
294 CGATCG
BsmFI 508 GTCCC GGGAC(10/14)
524 GTCCC
BsoBI 167 CCCGGG C^[CT]CG[GA]G
479 CCCGGG
BsrBI 240 CCGCTC CCGCTC(-3/-3)
583 CCGCTC
BsrFI 138 GCCGGC [GA]^CCGG[CT]
275 GCCGGC
Cfr10I 138 GCCGGC [GA]^CCGG[CT]
275 GCCGGC
DdeI 176 CTGAG C^T[ACGT]AG
561 CTCAG
DpnII 295 GATC ^GATC
454 GATC
EaeI 225 CGGCCG [CT]^GGCC[GA]
431 TGGCCG
FspI 266 TGCGCA TGC^GCA
344 TGCGCA
Hin1I 171 GGCGTC G[GA]^CG[CT]C
218 GGCGCC
HinfI 101 GACTC G^A[ACGT]TC
550 GAGTC
MboI 295 GATC ^GATC
454 GATC
MboII 88 GAAGA GAAGA(8/7)
616 GAAGA
MspA1I 51 CAGCGG C[AC]G^C[GT]G
222 CCGCGG
NaeI 138 GCCGGC GCC^GGC
275 GCCGGC
NdeII 295 GATC ^GATC
454 GATC
NgoMIV 138 GCCGGC G^CCGGC
275 GCCGGC
PleI 101 GACTC GAGTC(4/5)
550 GAGTC
Sau3AI 295 GATC ^GATC
454 GATC
SmaI 167 CCCGGG CCC^GGG
479 CCCGGG
TaqI 35 TCGA T^CGA
392 TCGA
TthHB8I 35 TCGA T^CGA
392 TCGA
XmaCI 167 CCCGGG C^CCGGG
479 CCCGGG
XmaI 167 CCCGGG C^CCGGG
479 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI BanI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MnlI
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI TseI TspRI