Restriction map for
pF1KB9628
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcsI AflII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I CfoI Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KspI
MaeII MamI MboI MfeI MflI
MluI MroI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeII
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI Sau3AI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 901 GGTACC G^GTACC
AcyI 313 GACGCC G[GA]^CG[CT]C
AflIII 929 ACATGT A^C[GA][CT]GT
AocI 327 CCTGAGG CC^T[ACGT]AGG
Asp718I 901 GGTACC G^GTACC
BanI 901 GGTACC G^G[CT][GA]CC
BbsI 982 GTCTTC GAAGAC(2/6)
BbvI 134 GCAGC GCAGC(8/12)
BlpI 129 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 129 GCTCAGC GC^T[ACGT]AGC
BpuAI 982 GTCTTC GAAGAC(2/6)
BsaHI 313 GACGCC G[GA]^CG[CT]C
BsaI 806 GGTCTC GGTCTC(1/5)
BsmI 879 GCATTC GAATGC(1/-1)
BspLU11I 929 ACATGT A^CATGT
BssSI 556 CACGAG CACGAG(-5/-1)
BstXI 23 CCAAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 327 CCTGAGG CC^T[ACGT]AGG
Cac8I 238 GCTCGC GC[ACGT]^[ACGT]GC
CelII 129 GCTCAGC GC^T[ACGT]AGC
Eco81I 327 CCTGAGG CC^T[ACGT]AGG
EcoT14I 23 CCAAGG C^C[AT][AT]GG
EcoT22I 298 ATGCAT ATGCA^T
Fnu4HI 134 GCAGC GC^[ACGT]GC
HgaI 313 GACGC GACGC(5/10)
Hin1I 313 GACGCC G[GA]^CG[CT]C
HincII 43 GTCAAC GT[CT]^[GA]AC
HindII 43 GTCAAC GT[CT]^[GA]AC
HphI 90 GGTGA GGTGA(8/7)
ItaI 134 GCAGC GC^[ACGT]GC
KpnI 901 GGTACC GGTAC^C
MseI 139 TTAA T^TAA
NdeI 646 CATATG CA^TATG
NsiI 298 ATGCAT ATGCA^T
PstI 729 CTGCAG CTGCA^G
SapI 145 GAAGAGC GCTCTTC(1/4)
StyI 23 CCAAGG C^C[AT][AT]GG
TaqI 261 TCGA T^CGA
Tru9I 139 TTAA T^TAA
TseI 134 GCAGC G^C[AT]GC
Tsp45I 578 GTGAC ^GT[GC]AC
TthHB8I 261 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 528 GGGCCC GGGCC^C
750 GGGCCC
BalI 392 TGGCCA TGG^CCA
435 TGGCCA
BcnI 293 CCCGG CC^[GC]GG
619 CCGGG
BpmI 427 CTGGAG CTGGAG(16/14)
547 CTGGAG
BsmFI 964 GGGAC GGGAC(10/14)
212 GTCCC
EaeI 392 TGGCCA [CT]^GGCC[GA]
435 TGGCCA
HapII 294 CCGG C^CGG
619 CCGG
HpaII 294 CCGG C^CGG
619 CCGG
MaeIII 578 GTGAC ^GT[ACGT]AC
911 GTTAC
MluNI 392 TGGCCA TGG^CCA
435 TGGCCA
MscI 392 TGGCCA TGG^CCA
435 TGGCCA
MspI 294 CCGG C^CGG
619 CCGG
MwoI 333 GCGAGTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
483 GCTCTCTCAGC
NciI 293 CCCGG CC^[GC]GG
619 CCGGG
NspI 873 GCATGT [GA]CATG^[CT]
929 ACATGT
PpuMI 210 GGGTCCC [GA]G^G[AT]CC[CT]
964 GGGACCC
SexAI 111 ACCAGGT A^CC[AT]GGT
503 ACCTGGT
SmlI 244 CTTGAG C^T[CT][GA]AG
670 CTCAAG
TspRI 777 CAGTG CA[GC]TG[ACGT][ACGT]^
987 CACTG
XcmI 494 CCACAAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
951 CCATCATGGTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI AvaII BanII BfaI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DraII EarI EcoO109I
EcoRII FokI HaeIII HinfI Ksp632I
MaeI MboII MnlI MvaI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfaNI SfcI TfiI