Restriction map for
pF1KB9615
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI ApaI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaII AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HgaI HincII
HindII HindIII HinfI HpaI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 678 GGTACC G^GTACC
AcyI 641 GGCGCC G[GA]^CG[CT]C
AlwI 697 GATCC GGATC(4/5)
AocI 310 CCTGAGG CC^T[ACGT]AGG
Aor51HI 455 AGCGCT AGC^GCT
Asp718I 678 GGTACC G^GTACC
BbeI 641 GGCGCC GGCGC^C
BcgI 558 GCACCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 264 TGATCA T^GATCA
BglI 89 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 641 GGCGCC G[GA]^CG[CT]C
BsaI 285 GGTCTC GGTCTC(1/5)
BsmFI 719 GGGAC GGGAC(10/14)
BspDI 259 ATCGAT AT^CGAT
BstAPI 431 GCAAGGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 696 AGATCC [GA]^GATC[CT]
Bsu36I 310 CCTGAGG CC^T[ACGT]AGG
ClaI 259 ATCGAT AT^CGAT
DraII 332 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eco47III 455 AGCGCT AGC^GCT
Eco81I 310 CCTGAGG CC^T[ACGT]AGG
EcoO109I 332 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 671 CCATGG C^C[AT][AT]GG
FbaI 264 TGATCA T^GATCA
Hin1I 641 GGCGCC G[GA]^CG[CT]C
HphI 213 GGTGA GGTGA(8/7)
KasI 641 GGCGCC G^GCGCC
KpnI 678 GGTACC GGTAC^C
MaeIII 214 GTGAC ^GT[ACGT]AC
MflI 696 AGATCC [GA]^GATC[CT]
NarI 641 GGCGCC GG^CGCC
NcoI 671 CCATGG C^CATGG
SfiI 88 GGCCGCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 641 GGCGCC GGC^GCC
SmlI 136 CTCAAG C^T[CT][GA]AG
StuI 140 AGGCCT AGG^CCT
StyI 671 CCATGG C^C[AT][AT]GG
TaqI 260 TCGA T^CGA
Tsp45I 214 GTGAC ^GT[GC]AC
TthHB8I 260 TCGA T^CGA
XhoII 696 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 538 CGCG CG^CG
568 CGCG
AfaI 591 GTAC GT^AC
679 GTAC
AluI 413 AGCT AG^CT
551 AGCT
Alw44I 571 GTGCAC G^TGCAC
689 GTGCAC
AlwNI 299 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
701 CAGGGTCTG
ApaLI 571 GTGCAC G^TGCAC
689 GTGCAC
AspHI 571 GTGCAC G[AT]GC[AT]^C
689 GTGCAC
BanI 641 GGCGCC G^G[CT][GA]CC
678 GGTACC
BanII 187 GAGCCC G[GA]GC[CT]^C
315 GGGCTC
BbvI 230 GCAGC GCAGC(8/12)
512 GCTGC
BpmI 459 CTCCAG CTGGAG(16/14)
385 CTGGAG
BsgI 199 GTGCAG GTGCAG(16/14)
205 GTGCAG
BsiHKAI 571 GTGCAC G[AT]GC[AT]^C
689 GTGCAC
BsmAI 766 GAGAC GTCTC(1/5)
286 GTCTC
BspMI 467 ACCTGC ACCTGC(4/8)
201 GCAGGT
BsrFI 493 GCCGGC [GA]^CCGG[CT]
786 GCCGGC
BstF5I 486 CATCC GGATG(2/0)
732 CATCC
BstUI 538 CGCG CG^CG
568 CGCG
Cfr10I 493 GCCGGC [GA]^CCGG[CT]
786 GCCGGC
DdeI 311 CTGAG C^T[ACGT]AG
408 CTCAG
DsaI 671 CCATGG C^C[GA][CT]GG
715 CCGTGG
EarI 49 GAAGAG CTCTTC(1/4)
757 GAAGAG
FokI 486 CATCC GGATG(9/13)
732 CATCC
Ksp632I 49 GAAGAG CTCTTC(1/4)
757 GAAGAG
MboII 49 GAAGA GAAGA(8/7)
757 GAAGA
MvnI 538 CGCG CG^CG
568 CGCG
NaeI 493 GCCGGC GCC^GGC
786 GCCGGC
NgoMIV 493 GCCGGC G^CCGGC
786 GCCGGC
RsaI 591 GTAC GT^AC
679 GTAC
SfaNI 398 GCATC GCATC(5/9)
731 GCATC
SmaI 40 CCCGGG CCC^GGG
83 CCCGGG
TseI 230 GCAGC G^C[AT]GC
512 GCTGC
XmaCI 40 CCCGGG C^CCGGG
83 CCCGGG
XmaI 40 CCCGGG C^CCGGG
83 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AvaI BcnI BmyI BsaJI
BseRI BsiYI BslI BsoBI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DpnII EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TspRI