Restriction map for
pF1KB9614
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI BstYI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRII EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MroI MspA1I MunI MvaI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AciI 1209 GCGG CCGC(-3/-1)
AhdI 1452 GACTTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 190 CCTCAGG CC^T[ACGT]AGG
AspEI 1452 GACTTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1464 GAGCTC G[AT]GC[AT]^C
AvaII 1211 GGACC G^G[AT]CC
BanI 557 GGCACC G^G[CT][GA]CC
BanII 1464 GAGCTC G[GA]GC[CT]^C
BcnI 255 CCGGG CC^[GC]GG
BfaI 367 CTAG C^TAG
BmyI 1464 GAGCTC G[AGT]GC[ACT]^C
BsaI 666 GAGACC GGTCTC(1/5)
BsiHKAI 1464 GAGCTC G[AT]GC[AT]^C
Bsp1286I 1464 GAGCTC G[AGT]GC[ACT]^C
BspHI 105 TCATGA T^CATGA
BsrDI 848 GCAATG GCAATG(2/0)
BssKI 255 CCGGG ^CC[ACGT]GG
Bsu36I 190 CCTCAGG CC^T[ACGT]AGG
Cac8I 1376 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 1211 GGACC G^G[ACGT]CC
DpnII 222 GATC ^GATC
DsaI 161 CCATGG C^C[GA][CT]GG
Eam1105I 1452 GACTTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 166 GAAGAG CTCTTC(1/4)
Eco81I 190 CCTCAGG CC^T[ACGT]AGG
Ksp632I 166 GAAGAG CTCTTC(1/4)
MaeI 367 CTAG C^TAG
MaeIII 304 GTTAC ^GT[ACGT]AC
MboI 222 GATC ^GATC
NciI 255 CCGGG CC^[GC]GG
NcoI 161 CCATGG C^CATGG
NdeII 222 GATC ^GATC
NlaIV 557 GGCACC GG[ACGT]^[ACGT]CC
PleI 157 GAGTC GAGTC(4/5)
PstI 282 CTGCAG CTGCA^G
RcaI 105 TCATGA T^CATGA
SacI 1464 GAGCTC GAGCT^C
Sau3AI 222 GATC ^GATC
Sau96I 1211 GGACC G^G[ACGT]CC
ScrFI 255 CCGGG CC^[ACGT]GG
SfcI 282 CTGCAG C^T[GA][CT]AG
SmlI 240 CTCAAG C^T[CT][GA]AG
XcmI 290 CCAGCCCAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 134 ACATGT A^C[GA][CT]GT
424 ACATGT
BalI 354 TGGCCA TGG^CCA
1038 TGGCCA
BbvI 281 GCTGC GCAGC(8/12)
1305 GCTGC
BseRI 188 CTCCTC GAGGAG(10/8)
1474 CTCCTC
BsiYI 294 CCCAAATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1118 CCTTCAGGGGG
BslI 294 CCCAAATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1118 CCTTCAGGGGG
BspLU11I 134 ACATGT A^CATGT
424 ACATGT
BstF5I 468 CATCC GGATG(2/0)
121 GGATG
EaeI 354 TGGCCA [CT]^GGCC[GA]
1038 TGGCCA
Fnu4HI 281 GCTGC GC^[ACGT]GC
1305 GCTGC
FokI 468 CATCC GGATG(9/13)
121 GGATG
HapII 255 CCGG C^CGG
975 CCGG
HpaII 255 CCGG C^CGG
975 CCGG
ItaI 281 GCTGC GC^[ACGT]GC
1305 GCTGC
MluNI 354 TGGCCA TGG^CCA
1038 TGGCCA
MscI 354 TGGCCA TGG^CCA
1038 TGGCCA
MseI 227 TTAA T^TAA
449 TTAA
MspI 255 CCGG C^CGG
975 CCGG
NdeI 475 CATATG CA^TATG
755 CATATG
NspI 134 ACATGT [GA]CATG^[CT]
424 ACATGT
SfaNI 810 GCATC GCATC(5/9)
978 GCATC
TaqI 151 TCGA T^CGA
552 TCGA
Tru9I 227 TTAA T^TAA
449 TTAA
TseI 281 GCTGC G^C[AT]GC
1305 GCTGC
TthHB8I 151 TCGA T^CGA
552 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BbsI
BpmI BpuAI BsaJI BsmAI BsmFI
BsmI BsrI DdeI EcoRI EcoT14I
HaeIII HinfI HphI MboII MnlI
MslI MwoI NlaIII StuI StyI
TfiI Tsp509I TspRI XmnI