Restriction map for
pF1KB9612
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsiYI BslI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I KasI
KpnI KspI MflI MluI MroI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SphI Sse8387I SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AflII 1027 CTTAAG C^TTAAG
AlwI 442 GATCC GGATC(4/5)
BalI 1019 TGGCCA TGG^CCA
BbvI 946 GCTGC GCAGC(8/12)
BcnI 1775 CCGGG CC^[GC]GG
BfrI 1027 CTTAAG C^TTAAG
BsaAI 1403 TACGTA [CT]AC^GT[GA]
BsaBI 1904 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1710 GGTCTC GGTCTC(1/5)
BsaJI 1390 CCAAGG C^C[ACGT][ACGT]GG
BsmFI 874 GTCCC GGGAC(10/14)
BsmI 1960 GAATGC GAATGC(1/-1)
Bsp1407I 484 TGTACA T^GTACA
BspMI 971 GCAGGT ACCTGC(4/8)
BsrGI 484 TGTACA T^GTACA
BstF5I 1263 CATCC GGATG(2/0)
BstXI 1225 CCATTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1032 GCGTGC GC[ACGT]^[ACGT]GC
Cfr13I 1502 GGCCC G^G[ACGT]CC
DraII 1501 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 1019 TGGCCA [CT]^GGCC[GA]
EcoO109I 1501 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1345 GAATTC G^AATTC
EcoT14I 1390 CCAAGG C^C[AT][AT]GG
Fnu4HI 946 GCTGC GC^[ACGT]GC
FokI 1263 CATCC GGATG(9/13)
HapII 1775 CCGG C^CGG
HgaI 261 GCGTC GACGC(5/10)
HindIII 1024 AAGCTT A^AGCTT
HpaI 1657 GTTAAC GTT^AAC
HpaII 1775 CCGG C^CGG
ItaI 946 GCTGC GC^[ACGT]GC
MaeII 1404 ACGT A^CGT
MamI 1904 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1190 CAATTG C^AATTG
MluNI 1019 TGGCCA TGG^CCA
MscI 1019 TGGCCA TGG^CCA
MspA1I 936 CAGCTG C[AC]G^C[GT]G
MspI 1775 CCGG C^CGG
MunI 1190 CAATTG C^AATTG
MwoI 727 GCTTTTCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1775 CCGGG CC^[GC]GG
NdeI 77 CATATG CA^TATG
PvuII 936 CAGCTG CAG^CTG
SacI 1128 GAGCTC GAGCT^C
Sau96I 1502 GGCCC G^G[ACGT]CC
SnaBI 1403 TACGTA TAC^GTA
SpeI 198 ACTAGT A^CTAGT
SspBI 484 TGTACA T^GTACA
StuI 556 AGGCCT AGG^CCT
StyI 1390 CCAAGG C^C[AT][AT]GG
TaqI 591 TCGA T^CGA
TseI 946 GCTGC G^C[AT]GC
TthHB8I 591 TCGA T^CGA
XcmI 1605 CCAAGTAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 429 GTGCTC G[AT]GC[AT]^C
1128 GAGCTC
BanII 734 GGGCTC G[GA]GC[CT]^C
1128 GAGCTC
BpmI 208 CTGGAG CTGGAG(16/14)
352 CTGGAG
BseRI 1994 CTCCTC GAGGAG(10/8)
835 GAGGAG
BsiHKAI 429 GTGCTC G[AT]GC[AT]^C
1128 GAGCTC
BsmAI 2007 GAGAC GTCTC(1/5)
1711 GTCTC
BstNI 1690 CCTGG CC^[AT]GG
1765 CCTGG
EarI 750 GAAGAG CTCTTC(1/4)
2004 GAAGAG
EcoRII 1690 CCTGG ^CC[AT]GG
1765 CCTGG
EcoT22I 479 ATGCAT ATGCA^T
1592 ATGCAT
HincII 597 GTCAAC GT[CT]^[GA]AC
1657 GTTAAC
HindII 597 GTCAAC GT[CT]^[GA]AC
1657 GTTAAC
Ksp632I 750 GAAGAG CTCTTC(1/4)
2004 GAAGAG
MslI 161 CATTTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1955 CATTTGAATG
MvaI 1690 CCTGG CC^[AT]GG
1765 CCTGG
NlaIV 1686 GGCTCC GG[ACGT]^[ACGT]CC
1992 GGCTCC
NsiI 479 ATGCAT ATGCA^T
1592 ATGCAT
NspI 481 GCATGT [GA]CATG^[CT]
531 GCATGT
SmlI 1027 CTTAAG C^T[CT][GA]AG
1095 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BfaI BmyI BpuAI Bsp1286I BsrI
BssKI DdeI DpnII DraI HaeIII
HinfI HphI MaeI MaeIII MboI
MboII MnlI MseI NdeII NlaIII
PleI RsaI Sau3AI ScrFI SfaNI
SspI TfiI Tru9I Tsp45I Tsp509I
TspRI