Restriction map for
pF1KB9609
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AlwI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AvaII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsiEI BsiWI BsiYI BslI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstBI BstEII BstF5I
BstNI BstPI BstUI BstYI BstZ17I
Bsu36I Cac8I CelII Cfr10I Cfr13I
ClaI CpoI DraI DraII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoRV EcoT14I EcoT22I FbaI
FokI FseI HapII HgaI Hin1I
HindIII HpaI HpaII KasI KpnI
KspI MaeI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MspI MunI MvaI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI Sau96I SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AciI 653 GCGG CCGC(-3/-1)
AhdI 324 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 247 GTGCAC G^TGCAC
ApaLI 247 GTGCAC G^TGCAC
AspEI 324 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 247 GTGCAC G[AT]GC[AT]^C
AviII 501 TGCGCA TGC^GCA
BbsI 255 GTCTTC GAAGAC(2/6)
BpuAI 255 GTCTTC GAAGAC(2/6)
BsiHKAI 247 GTGCAC G[AT]GC[AT]^C
BsmAI 85 GTCTC GTCTC(1/5)
BstAPI 644 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 561 CCATGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 87 CTCAG C^T[ACGT]AG
DpnII 100 GATC ^GATC
Eam1105I 324 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 653 GCGGG CCCGC(4/6)
FspI 501 TGCGCA TGC^GCA
HaeII 415 AGCGCC [GA]GCGC^[CT]
HincII 602 GTCAAC GT[CT]^[GA]AC
HindII 602 GTCAAC GT[CT]^[GA]AC
HinfI 3 GACTC G^A[ACGT]TC
MboI 100 GATC ^GATC
MnlI 590 CCTC CCTC(7/6)
MseI 581 TTAA T^TAA
NdeII 100 GATC ^GATC
NspI 556 ACATGC [GA]CATG^[CT]
PleI 3 GACTC GAGTC(4/5)
PstI 669 CTGCAG CTGCA^G
SapI 152 GAAGAGC GCTCTTC(1/4)
Sau3AI 100 GATC ^GATC
TaqI 687 TCGA T^CGA
Tru9I 581 TTAA T^TAA
TthHB8I 687 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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BbvI 671 GCAGC GCAGC(8/12)
668 GCTGC
BmyI 170 GTGCCC G[AGT]GC[ACT]^C
247 GTGCAC
BpmI 203 CTGGAG CTGGAG(16/14)
287 CTGGAG
BsgI 352 CTGCAC GTGCAG(16/14)
642 GTGCAG
BsmI 52 GAATGC GAATGC(1/-1)
388 GAATGC
Bsp1286I 170 GTGCCC G[AGT]GC[ACT]^C
247 GTGCAC
CfoI 416 GCGC GCG^C
502 GCGC
DraIII 449 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
533 CACACAGTG
EarI 507 CTCTTC CTCTTC(1/4)
152 GAAGAG
Fnu4HI 668 GCTGC GC^[ACGT]GC
671 GCAGC
HaeIII 72 GGCC GG^CC
576 GGCC
HhaI 416 GCGC GCG^C
502 GCGC
HinP1I 416 GCGC G^CGC
502 GCGC
ItaI 668 GCTGC GC^[ACGT]GC
671 GCAGC
Ksp632I 507 CTCTTC CTCTTC(1/4)
152 GAAGAG
MaeIII 433 GTTAC ^GT[ACGT]AC
517 GTTAC
MwoI 493 GCCTTCAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
644 GCAGTCAATGC
SfaNI 339 GATGC GCATC(5/9)
18 GCATC
SfcI 669 CTGCAG C^T[GA][CT]AG
693 CTGTAG
StuI 71 AGGCCT AGG^CCT
575 AGGCCT
TseI 668 GCTGC G^C[AT]GC
671 GCAGC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
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AcsI AluI ApoI BsrI EcoRI
HphI MboII MslI NlaIII Tsp509I
TspRI