Restriction map for
pF1KB9608
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AhdI AlwNI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII BalI BanI
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BfrI BglI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I TseI
Tsp509I Tth111I Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AgeI 755 ACCGGT A^CCGGT
AocI 40 CCTGAGG CC^T[ACGT]AGG
AvrII 486 CCTAGG C^CTAGG
BlnI 486 CCTAGG C^CTAGG
BsaI 663 GAGACC GGTCTC(1/5)
BsmAI 663 GAGAC GTCTC(1/5)
BsmFI 206 GGGAC GGGAC(10/14)
BspMI 35 ACCTGC ACCTGC(4/8)
BsrBI 391 GAGCGG CCGCTC(-3/-3)
BsrDI 213 CATTGC GCAATG(2/0)
BsrFI 755 ACCGGT [GA]^CCGG[CT]
BssSI 388 CACGAG CACGAG(-5/-1)
Bsu36I 40 CCTGAGG CC^T[ACGT]AGG
CfoI 453 GCGC GCG^C
Cfr10I 755 ACCGGT [GA]^CCGG[CT]
DraII 206 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 169 CCATGG C^C[GA][CT]GG
EaeI 496 CGGCCA [CT]^GGCC[GA]
Eco81I 40 CCTGAGG CC^T[ACGT]AGG
EcoO109I 206 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 703 GATATC GAT^ATC
HaeII 452 GGCGCT [GA]GCGC^[CT]
HaeIII 497 GGCC GG^CC
HhaI 453 GCGC GCG^C
HinP1I 453 GCGC G^CGC
NcoI 169 CCATGG C^CATGG
NheI 546 GCTAGC G^CTAGC
PinAI 755 ACCGGT A^CCGGT
PpuMI 206 GGGACCC [GA]G^G[AT]CC[CT]
SacI 789 GAGCTC GAGCT^C
SapI 606 GAAGAGC GCTCTTC(1/4)
XcmI 160 CCAAAAAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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Alw44I 675 GTGCAC G^TGCAC
759 GTGCAC
ApaLI 675 GTGCAC G^TGCAC
759 GTGCAC
Asp700I 606 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
774 GAAACACTTC
AvaI 194 CTCGAG C^[CT]CG[GA]G
363 CTCGAG
BanII 191 GGGCTC G[GA]GC[CT]^C
789 GAGCTC
BcnI 189 CCGGG CC^[GC]GG
785 CCCGG
BpmI 624 CTCCAG CTGGAG(16/14)
571 CTGGAG
BsaWI 560 ACCGGA [AT]^CCGG[AT]
755 ACCGGT
BsoBI 194 CTCGAG C^[CT]CG[GA]G
363 CTCGAG
BsrI 319 ACTGG ACTGG(1/-1)
261 CCAGT
DraIII 678 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
762 CACTGTGTG
EarI 406 GAAGAG CTCTTC(1/4)
606 GAAGAG
EcoNI 36 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
40 CCTGAGGAAGG
Fnu4HI 450 GCGGC GC^[ACGT]GC
495 GCGGC
ItaI 450 GCGGC GC^[ACGT]GC
495 GCGGC
Ksp632I 406 GAAGAG CTCTTC(1/4)
606 GAAGAG
MaeIII 181 GTCAC ^GT[ACGT]AC
509 GTGAC
MboII 406 GAAGA GAAGA(8/7)
606 GAAGA
MslI 316 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
652 CACACAGGTG
NciI 189 CCGGG CC^[GC]GG
785 CCCGG
PaeR7I 194 CTCGAG C^TCGAG
363 CTCGAG
PleI 79 GACTC GAGTC(4/5)
21 GAGTC
SmlI 194 CTCGAG C^T[CT][GA]AG
363 CTCGAG
TfiI 795 GAATC G^A[AT]TC
844 GATTC
Tsp45I 181 GTCAC ^GT[GC]AC
509 GTGAC
XhoI 194 CTCGAG C^TCGAG
363 CTCGAG
XmnI 606 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
774 GAAACACTTC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BamHI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I FokI HapII
HinfI HpaII HphI MaeI MboI
MflI MnlI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TspRI TthHB8I XhoII