Restriction map for
pF1KB9607
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AocI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrDI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 291 GTAC GT^AC
AflIII 293 ACATGT A^C[GA][CT]GT
AhdI 202 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 496 GTGCAC G^TGCAC
AlwI 118 GGATC GGATC(4/5)
ApaI 63 GGGCCC GGGCC^C
ApaLI 496 GTGCAC G^TGCAC
Asp700I 363 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 202 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 100 GGACC G^G[AT]CC
BanI 395 GGCACC G^G[CT][GA]CC
BbsI 283 GTCTTC GAAGAC(2/6)
BbvCI 25 CCTCAGC CCTCAGC(-5/-2)
BglI 229 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 239 CTCCAG CTGGAG(16/14)
BpuAI 283 GTCTTC GAAGAC(2/6)
BsaI 52 GAGACC GGTCTC(1/5)
BsmAI 52 GAGAC GTCTC(1/5)
BsmFI 181 GTCCC GGGAC(10/14)
BsmI 367 GCATTC GAATGC(1/-1)
BspLU11I 293 ACATGT A^CATGT
BspMI 158 ACCTGC ACCTGC(4/8)
BsrI 230 CCAGT ACTGG(1/-1)
BssHII 375 GCGCGC G^CGCGC
BstYI 118 GGATCT [GA]^GATC[CT]
DpnII 119 GATC ^GATC
DraIII 573 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 202 GACGAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HinfI 208 GAGTC G^A[ACGT]TC
MboI 119 GATC ^GATC
MboII 284 TCTTC GAAGA(8/7)
MflI 118 GGATCT [GA]^GATC[CT]
MspA1I 261 CAGCTG C[AC]G^C[GT]G
NcoI 56 CCATGG C^CATGG
NdeII 119 GATC ^GATC
NspI 293 ACATGT [GA]CATG^[CT]
PflMI 91 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 208 GAGTC GAGTC(4/5)
PstI 562 CTGCAG CTGCA^G
PvuII 261 CAGCTG CAG^CTG
RsaI 291 GTAC GT^AC
Sau3AI 119 GATC ^GATC
SmlI 276 CTCAAG C^T[CT][GA]AG
Van91I 91 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 118 GGATCT [GA]^GATC[CT]
XmnI 363 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccII 376 CGCG CG^CG
575 CGCG
AluI 262 AGCT AG^CT
476 AGCT
AlwNI 28 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
92 CAGAACCTG
AspHI 273 GTGCTC G[AT]GC[AT]^C
496 GTGCAC
BanII 63 GGGCCC G[GA]GC[CT]^C
236 GGGCTC
BcnI 462 CCGGG CC^[GC]GG
492 CCGGG
BsiHKAI 273 GTGCTC G[AT]GC[AT]^C
496 GTGCAC
BsrBI 84 CCGCTC CCGCTC(-3/-3)
442 CCGCTC
BstUI 376 CGCG CG^CG
575 CGCG
BstXI 380 CCAGCCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
404 CCATTCACCTGG
CfoI 375 GCGC GCG^C
377 GCGC
DdeI 26 CTCAG C^T[ACGT]AG
173 CTCAG
DraII 62 AGGGCCC [GA]G^G[ACGT]CC[CT]
63 GGGCCCT
DsaI 56 CCATGG C^C[GA][CT]GG
432 CCACGG
EcoO109I 62 AGGGCCC [GA]G^G[ACGT]CC[CT]
63 GGGCCCT
EcoT14I 56 CCATGG C^C[AT][AT]GG
142 CCAAGG
HapII 462 CCGG C^CGG
492 CCGG
HhaI 375 GCGC GCG^C
377 GCGC
HinP1I 375 GCGC G^CGC
377 GCGC
HpaII 462 CCGG C^CGG
492 CCGG
MaeIII 210 GTCAC ^GT[ACGT]AC
350 GTGAC
MspI 462 CCGG C^CGG
492 CCGG
MvnI 376 CGCG CG^CG
575 CGCG
NciI 462 CCGGG CC^[GC]GG
492 CCGGG
NlaIII 57 CATG CATG^
294 CATG
SfcI 258 CTACAG C^T[GA][CT]AG
562 CTGCAG
StyI 56 CCATGG C^C[AT][AT]GG
142 CCAAGG
TaqI 287 TCGA T^CGA
344 TCGA
Tsp45I 210 GTCAC ^GT[GC]AC
350 GTGAC
TthHB8I 287 TCGA T^CGA
344 TCGA
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI BbvI BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I EcoRII FauI
Fnu4HI FokI HaeIII HphI ItaI
MnlI MvaI MwoI NlaIV Sau96I
ScrFI SfaNI TseI TspRI