Restriction map for
pF1KB9599
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbeI BbrPI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspEI BspHI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CpoI DraI DraIII Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV FbaI FseI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I TspRI Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 557 ACATGT A^C[GA][CT]GT
AlwI 492 GATCC GGATC(4/5)
AlwNI 1003 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 591 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 202 GAGCAC G[AT]GC[AT]^C
AviII 1010 TGCGCA TGC^GCA
BalI 344 TGGCCA TGG^CCA
BbvCI 900 CCTCAGC CCTCAGC(-5/-2)
BcgI 783 GCATCCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 139 CTAG C^TAG
BsiHKAI 202 GAGCAC G[AT]GC[AT]^C
BsmAI 756 GAGAC GTCTC(1/5)
BsmBI 756 GAGACG CGTCTC(1/5)
BspDI 79 ATCGAT AT^CGAT
BspLU11I 557 ACATGT A^CATGT
BsrBI 994 GAGCGG CCGCTC(-3/-3)
BsrFI 303 GCCGGC [GA]^CCGG[CT]
BstYI 491 AGATCC [GA]^GATC[CT]
Cfr10I 303 GCCGGC [GA]^CCGG[CT]
ClaI 79 ATCGAT AT^CGAT
DpnII 492 GATC ^GATC
DrdI 478 GACTGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 341 CCATGG C^C[GA][CT]GG
EagI 1169 CGGCCG C^GGCCG
EclXI 1169 CGGCCG C^GGCCG
Eco52I 1169 CGGCCG C^GGCCG
EcoNI 87 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 950 ATGCAT ATGCA^T
FspI 1010 TGCGCA TGC^GCA
MaeI 139 CTAG C^TAG
MboI 492 GATC ^GATC
MflI 491 AGATCC [GA]^GATC[CT]
MluNI 344 TGGCCA TGG^CCA
MscI 344 TGGCCA TGG^CCA
MslI 336 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 303 GCCGGC GCC^GGC
NcoI 341 CCATGG C^CATGG
NdeII 492 GATC ^GATC
NgoMIV 303 GCCGGC G^CCGGC
NsiI 950 ATGCAT ATGCA^T
NspI 557 ACATGT [GA]CATG^[CT]
PpuMI 1252 GGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 492 GATC ^GATC
Tsp509I 1080 AATT ^AATT
XhoII 491 AGATCC [GA]^GATC[CT]
XmnI 591 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 758 GACGCC G[GA]^CG[CT]C
929 GGCGTC
AfaI 324 GTAC GT^AC
1000 GTAC
ApaI 701 GGGCCC GGGCC^C
1309 GGGCCC
BbsI 67 GAAGAC GAAGAC(2/6)
106 GAAGAC
BglI 509 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1171 GCCGCGTCGGC
BlpI 16 GCTGAGC GC^T[ACGT]AGC
378 GCTCAGC
Bpu1102I 16 GCTGAGC GC^T[ACGT]AGC
378 GCTCAGC
BpuAI 67 GAAGAC GAAGAC(2/6)
106 GAAGAC
BsaHI 758 GACGCC G[GA]^CG[CT]C
929 GGCGTC
CelII 16 GCTGAGC GC^T[ACGT]AGC
378 GCTCAGC
Hin1I 758 GACGCC G[GA]^CG[CT]C
929 GGCGTC
HinfI 55 GACTC G^A[ACGT]TC
1300 GAGTC
HphI 338 TCACC GGTGA(8/7)
1258 TCACC
MspA1I 381 CAGCGG C[AC]G^C[GT]G
1096 CAGCGG
PleI 55 GACTC GAGTC(4/5)
1300 GAGTC
PstI 163 CTGCAG CTGCA^G
882 CTGCAG
RsaI 324 GTAC GT^AC
1000 GTAC
SfcI 163 CTGCAG C^T[GA][CT]AG
882 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI MboII MnlI MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI SmaI StyI
TaqI TseI TthHB8I XmaCI XmaI