Restriction map for
pF1KB9598
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII Alw44I AocI ApaLI ApoI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BbrPI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsmAI
BsmBI BsmI Bsp1407I BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I DraI DraIII
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KpnI MaeIII
MamI MfeI MluI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 1357 TCCGGA T^CCGGA
AgeI 933 ACCGGT A^CCGGT
AhdI 652 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 603 GATCC GGATC(4/5)
Aor51HI 1278 AGCGCT AGC^GCT
ApaI 1158 GGGCCC GGGCC^C
AscI 289 GGCGCGCC GG^CGCGCC
Asp700I 702 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 652 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 727 GAGCAC G[AT]GC[AT]^C
BbvCI 211 CCTCAGC CCTCAGC(-5/-2)
BfaI 415 CTAG C^TAG
BlpI 1308 GCTGAGC GC^T[ACGT]AGC
BpmI 901 CTCCAG CTGGAG(16/14)
Bpu1102I 1308 GCTGAGC GC^T[ACGT]AGC
BseAI 1357 TCCGGA T^CCGGA
BseRI 331 GAGGAG GAGGAG(10/8)
BsiHKAI 727 GAGCAC G[AT]GC[AT]^C
BsiWI 911 CGTACG C^GTACG
BspDI 73 ATCGAT AT^CGAT
BspEI 1357 TCCGGA T^CCGGA
BspHI 752 TCATGA T^CATGA
BsrDI 239 CATTGC GCAATG(2/0)
BstYI 602 AGATCC [GA]^GATC[CT]
CelII 1308 GCTGAGC GC^T[ACGT]AGC
ClaI 73 ATCGAT AT^CGAT
CpoI 270 CGGACCG CG^G[AT]CCG
DpnII 603 GATC ^GATC
Eam1105I 652 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 106 GAAGAG CTCTTC(1/4)
Eco47III 1278 AGCGCT AGC^GCT
HphI 449 TCACC GGTGA(8/7)
Ksp632I 106 GAAGAG CTCTTC(1/4)
MaeI 415 CTAG C^TAG
MboI 603 GATC ^GATC
MflI 602 AGATCC [GA]^GATC[CT]
MroI 1357 TCCGGA T^CCGGA
NcoI 452 CCATGG C^CATGG
NdeII 603 GATC ^GATC
PinAI 933 ACCGGT A^CCGGT
PpuMI 804 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1024 CAGCTG CAG^CTG
RcaI 752 TCATGA T^CATGA
RsrII 270 CGGACCG CG^G[AT]CCG
Sau3AI 603 GATC ^GATC
SmlI 1412 CTCAAG C^T[CT][GA]AG
StuI 384 AGGCCT AGG^CCT
TspRI 1209 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 602 AGATCC [GA]^GATC[CT]
XmnI 702 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 32 ACATGT A^C[GA][CT]GT
668 ACATGT
AlwNI 229 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1363 CAGTTTCTG
BalI 455 TGGCCA TGG^CCA
1286 TGGCCA
BglI 818 GCCCCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1048 GCCGCCGCGGC
BsaWI 933 ACCGGT [AT]^CCGG[AT]
1357 TCCGGA
BsgI 829 CTGCAC GTGCAG(16/14)
1267 GTGCAG
BsmFI 804 GGGAC GGGAC(10/14)
1261 GGGAC
BspLU11I 32 ACATGT A^CATGT
668 ACATGT
BspMI 731 ACCTGC ACCTGC(4/8)
124 GCAGGT
BssHII 290 GCGCGC G^CGCGC
1118 GCGCGC
BstF5I 459 CATCC GGATG(2/0)
564 CATCC
DrdI 589 GACTGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1263 GACCGTGCAGTC
EcoNI 162 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
527 CCTACTACAGG
EcoT14I 245 CCAAGG C^C[AT][AT]GG
452 CCATGG
FokI 459 CATCC GGATG(9/13)
564 CATCC
HgaI 121 GACGC GACGC(5/10)
1093 GCGTC
MaeII 65 ACGT A^CGT
284 ACGT
MboII 106 GAAGA GAAGA(8/7)
477 GAAGA
MluNI 455 TGGCCA TGG^CCA
1286 TGGCCA
MscI 455 TGGCCA TGG^CCA
1286 TGGCCA
MslI 72 CATCGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
447 CATCACCATG
NspI 32 ACATGT [GA]CATG^[CT]
668 ACATGT
StyI 245 CCAAGG C^C[AT][AT]GG
452 CCATGG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaJI
BsiEI BsiYI BslI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI KasI
KspI MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NgoMIV NlaIII NlaIV NotI PstI
RsaI SacII Sau96I ScrFI SfaNI
SfcI SfoI SgrAI SmaI TaqI
TseI TthHB8I XmaCI XmaI