Restriction map for
pF1KB9593
##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BseRI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MluI MroI MslI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 241 CGCG CG^CG
AcsI 765 GAATTT [GA]^AATT[CT]
AflIII 299 ACATGT A^C[GA][CT]GT
AlwI 478 GATCC GGATC(4/5)
AocI 399 CCTCAGG CC^T[ACGT]AGG
ApoI 765 GAATTT [GA]^AATT[CT]
BbeI 28 GGCGCC GGCGC^C
BglI 177 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 842 CTCCAG CTGGAG(16/14)
BsaWI 962 ACCGGA [AT]^CCGG[AT]
BsgI 827 CTGCAC GTGCAG(16/14)
BsiEI 440 CGACCG CG[GA][CT]^CG
BspLU11I 299 ACATGT A^CATGT
BspMI 901 GCAGGT ACCTGC(4/8)
BstUI 241 CGCG CG^CG
BstYI 477 AGATCC [GA]^GATC[CT]
Bsu36I 399 CCTCAGG CC^T[ACGT]AGG
CfoI 29 GCGC GCG^C
DpnII 478 GATC ^GATC
DraIII 386 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 418 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 399 CCTCAGG CC^T[ACGT]AGG
HaeII 28 GGCGCC [GA]GCGC^[CT]
HhaI 29 GCGC GCG^C
HinP1I 29 GCGC G^CGC
HindIII 492 AAGCTT A^AGCTT
KasI 28 GGCGCC G^GCGCC
MaeIII 210 GTCAC ^GT[ACGT]AC
MboI 478 GATC ^GATC
MflI 477 AGATCC [GA]^GATC[CT]
MseI 689 TTAA T^TAA
MspA1I 109 CAGCGG C[AC]G^C[GT]G
MvnI 241 CGCG CG^CG
NaeI 664 GCCGGC GCC^GGC
NarI 28 GGCGCC GG^CGCC
NcoI 566 CCATGG C^CATGG
NdeII 478 GATC ^GATC
NgoMIV 664 GCCGGC G^CCGGC
PflMI 646 CCAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 425 GAGTC GAGTC(4/5)
PpuMI 416 AGGACCC [GA]G^G[AT]CC[CT]
PstI 754 CTGCAG CTGCA^G
Sau3AI 478 GATC ^GATC
SfoI 28 GGCGCC GGC^GCC
SmlI 793 CTTGAG C^T[CT][GA]AG
SphI 601 GCATGC GCATG^C
Tru9I 689 TTAA T^TAA
Tsp45I 210 GTCAC ^GT[GC]AC
Tsp509I 766 AATT ^AATT
Van91I 646 CCAGCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 477 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 28 GGCGCC G[GA]^CG[CT]C
966 GACGCC
ApaI 372 GGGCCC GGGCC^C
857 GGGCCC
AvaI 19 CCCGGG C^[CT]CG[GA]G
368 CCCGGG
BalI 86 TGGCCA TGG^CCA
623 TGGCCA
BbvCI 809 CCTCAGC CCTCAGC(-5/-2)
358 GCTGAGG
BsaHI 28 GGCGCC G[GA]^CG[CT]C
966 GACGCC
BsmFI 427 GTCCC GGGAC(10/14)
714 GTCCC
BsoBI 19 CCCGGG C^[CT]CG[GA]G
368 CCCGGG
BstAPI 9 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
446 GCAGGCAGTGC
BstF5I 90 CATCC GGATG(2/0)
195 CATCC
FokI 90 CATCC GGATG(9/13)
195 CATCC
HgaI 276 GACGC GACGC(5/10)
966 GACGC
Hin1I 28 GGCGCC G[GA]^CG[CT]C
966 GACGCC
HphI 100 TCACC GGTGA(8/7)
341 TCACC
MboII 634 TCTTC GAAGA(8/7)
980 TCTTC
MluNI 86 TGGCCA TGG^CCA
623 TGGCCA
MscI 86 TGGCCA TGG^CCA
623 TGGCCA
NspI 299 ACATGT [GA]CATG^[CT]
601 GCATGC
SacI 96 GAGCTC GAGCT^C
613 GAGCTC
SfcI 60 CTACAG C^T[GA][CT]AG
754 CTGCAG
SmaI 19 CCCGGG CCC^GGG
368 CCCGGG
StuI 553 AGGCCT AGG^CCT
789 AGGCCT
TfiI 152 GATTC G^A[AT]TC
721 GAATC
XmaCI 19 CCCGGG C^CCGGG
368 CCCGGG
XmaI 19 CCCGGG C^CCGGG
368 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BanI BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrFI BsrI BssKI BstNI Cac8I
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MnlI MspI MvaI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
SfaNI StyI TseI TspRI