Restriction map for
pF1KB9590
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BseAI BsiHKAI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 954 AAATTT [GA]^AATT[CT]
AlwI 1114 GGATC GGATC(4/5)
ApoI 954 AAATTT [GA]^AATT[CT]
AvaI 109 CTCGGG C^[CT]CG[GA]G
BcgI 1085 CGATTGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 910 CTAG C^TAG
BmyI 760 GTGCCC G[AGT]GC[ACT]^C
BsaWI 1108 TCCGGT [AT]^CCGG[AT]
BsgI 364 GTGCAG GTGCAG(16/14)
BsiEI 733 CGGCCG CG[GA][CT]^CG
BsiWI 980 CGTACG C^GTACG
BsmBI 358 GAGACG CGTCTC(1/5)
BsmI 264 GCATTC GAATGC(1/-1)
BsoBI 109 CTCGGG C^[CT]CG[GA]G
Bsp1286I 760 GTGCCC G[AGT]GC[ACT]^C
BsrBI 106 CCGCTC CCGCTC(-3/-3)
BsrDI 1080 CATTGC GCAATG(2/0)
BstAPI 117 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 950 CCAGAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1114 GGATCT [GA]^GATC[CT]
CfoI 486 GCGC GCG^C
DsaI 77 CCGTGG C^C[GA][CT]GG
EagI 733 CGGCCG C^GGCCG
EclXI 733 CGGCCG C^GGCCG
Eco52I 733 CGGCCG C^GGCCG
EcoNI 211 CCTGTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 283 CCTTGG C^C[AT][AT]GG
FauI 105 CCCGC CCCGC(4/6)
HhaI 486 GCGC GCG^C
HinP1I 486 GCGC G^CGC
HincII 151 GTCAAC GT[CT]^[GA]AC
HindII 151 GTCAAC GT[CT]^[GA]AC
MaeI 910 CTAG C^TAG
MflI 1114 GGATCT [GA]^GATC[CT]
NotI 732 GCGGCCGC GC^GGCCGC
PstI 804 CTGCAG CTGCA^G
SexAI 689 ACCTGGT A^CC[AT]GGT
SmlI 40 CTCAAG C^T[CT][GA]AG
StyI 283 CCTTGG C^C[AT][AT]GG
TaqI 1154 TCGA T^CGA
TthHB8I 1154 TCGA T^CGA
XcmI 68 CCAGCCGCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1114 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 981 GTAC GT^AC
1122 GTAC
AvaII 377 GGACC G^G[AT]CC
960 GGTCC
BbrPI 757 CACGTG CAC^GTG
1050 CACGTG
BbsI 789 GAAGAC GAAGAC(2/6)
934 GAAGAC
BcnI 290 CCGGG CC^[GC]GG
917 CCGGG
BpuAI 789 GAAGAC GAAGAC(2/6)
934 GAAGAC
BsaI 189 GAGACC GGTCTC(1/5)
1141 GAGACC
BsmFI 376 GGGAC GGGAC(10/14)
961 GTCCC
BspMI 720 GCAGGT ACCTGC(4/8)
1030 GCAGGT
DpnII 549 GATC ^GATC
1115 GATC
EarI 615 GAAGAG CTCTTC(1/4)
1162 GAAGAG
HinfI 454 GAGTC G^A[ACGT]TC
913 GAGTC
HphI 277 TCACC GGTGA(8/7)
445 TCACC
Ksp632I 615 GAAGAG CTCTTC(1/4)
1162 GAAGAG
MaeIII 985 GTGAC ^GT[ACGT]AC
1067 GTGAC
MboI 549 GATC ^GATC
1115 GATC
MspA1I 730 CAGCGG C[AC]G^C[GT]G
736 CCGCTG
NciI 290 CCGGG CC^[GC]GG
917 CCGGG
NdeII 549 GATC ^GATC
1115 GATC
PleI 454 GAGTC GAGTC(4/5)
913 GAGTC
PmaCI 757 CACGTG CAC^GTG
1050 CACGTG
PmlI 757 CACGTG CAC^GTG
1050 CACGTG
RsaI 981 GTAC GT^AC
1122 GTAC
Sau3AI 549 GATC ^GATC
1115 GATC
SfcI 667 CTACAG C^T[GA][CT]AG
804 CTGCAG
Tsp45I 985 GTGAC ^GT[GC]AC
1067 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI BalI BbvI BpmI
BsaAI BsaJI BseRI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EaeI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MaeII MboII MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NlaIII NlaIV PflMI Sau96I
ScrFI SfaNI Tru9I TseI Tsp509I
TspRI Van91I