Restriction map for
pF1KB9588
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BcnI
BglI BlnI BpmI BpuAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MamI MluI
MluNI MroI MscI MslI MspI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI Tsp45I TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AciI 168 CCGC CCGC(-3/-1)
AcsI 618 AAATTC [GA]^AATT[CT]
AflII 334 CTTAAG C^TTAAG
AflIII 351 ACACGT A^C[GA][CT]GT
Alw44I 23 GTGCAC G^TGCAC
ApaLI 23 GTGCAC G^TGCAC
ApoI 618 AAATTC [GA]^AATT[CT]
AseI 259 ATTAAT AT^TAAT
AsnI 259 ATTAAT AT^TAAT
AspHI 23 GTGCAC G[AT]GC[AT]^C
BanII 294 GAGCCC G[GA]GC[CT]^C
BbrPI 352 CACGTG CAC^GTG
BclI 216 TGATCA T^GATCA
BfaI 325 CTAG C^TAG
BfrI 334 CTTAAG C^TTAAG
BglII 330 AGATCT A^GATCT
BlpI 86 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 86 GCTTAGC GC^T[ACGT]AGC
BsaAI 352 CACGTG [CT]AC^GT[GA]
BsaI 397 GAGACC GGTCTC(1/5)
BsiHKAI 23 GTGCAC G[AT]GC[AT]^C
BsiYI 168 CCGCCCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 168 CCGCCCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 397 GAGAC GTCTC(1/5)
Bsp1407I 348 TGTACA T^GTACA
BspHI 410 TCATGA T^CATGA
BsrGI 348 TGTACA T^GTACA
BsrI 592 ACTGG ACTGG(1/-1)
BssSI 220 CACGAG CACGAG(-5/-1)
BstYI 330 AGATCT [GA]^GATC[CT]
CelII 86 GCTTAGC GC^T[ACGT]AGC
EarI 316 GAAGAG CTCTTC(1/4)
FbaI 216 TGATCA T^GATCA
HincII 431 GTCAAC GT[CT]^[GA]AC
HindII 431 GTCAAC GT[CT]^[GA]AC
HphI 4 GGTGA GGTGA(8/7)
Ksp632I 316 GAAGAG CTCTTC(1/4)
MaeI 325 CTAG C^TAG
MaeIII 122 GTTAC ^GT[ACGT]AC
MfeI 585 CAATTG C^AATTG
MflI 330 AGATCT [GA]^GATC[CT]
MspA1I 446 CAGCTG C[AC]G^C[GT]G
MunI 585 CAATTG C^AATTG
NlaIV 293 GGAGCC GG[ACGT]^[ACGT]CC
PleI 407 GAGTC GAGTC(4/5)
PmaCI 352 CACGTG CAC^GTG
PmlI 352 CACGTG CAC^GTG
PshBI 259 ATTAAT AT^TAAT
PvuII 446 CAGCTG CAG^CTG
RcaI 410 TCATGA T^CATGA
SspBI 348 TGTACA T^GTACA
SspI 73 AATATT AAT^ATT
TaqI 191 TCGA T^CGA
TfiI 489 GATTC G^A[AT]TC
TthHB8I 191 TCGA T^CGA
XhoII 330 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 349 GTAC GT^AC
590 GTAC
AvaII 270 GGACC G^G[AT]CC
420 GGTCC
BbvI 339 GCTGC GCAGC(8/12)
658 GCTGC
BmyI 23 GTGCAC G[AGT]GC[ACT]^C
294 GAGCCC
BsaJI 297 CCCTGG C^C[ACGT][ACGT]GG
298 CCTGGG
Bsp1286I 23 GTGCAC G[AGT]GC[ACT]^C
294 GAGCCC
BssKI 298 CCTGG ^CC[ACGT]GG
468 CCTGG
BstNI 298 CCTGG CC^[AT]GG
468 CCTGG
Cfr13I 270 GGACC G^G[ACGT]CC
420 GGTCC
EcoRII 298 CCTGG ^CC[AT]GG
468 CCTGG
Fnu4HI 339 GCTGC GC^[ACGT]GC
658 GCTGC
HinfI 407 GAGTC G^A[ACGT]TC
489 GATTC
ItaI 339 GCTGC GC^[ACGT]GC
658 GCTGC
MaeII 353 ACGT A^CGT
429 ACGT
MboII 316 GAAGA GAAGA(8/7)
328 GAAGA
MvaI 298 CCTGG CC^[AT]GG
468 CCTGG
NlaIII 126 CATG CATG^
411 CATG
RsaI 349 GTAC GT^AC
590 GTAC
Sau96I 270 GGACC G^G[ACGT]CC
420 GGTCC
ScrFI 298 CCTGG CC^[ACGT]GG
468 CCTGG
SmlI 334 CTTAAG C^T[CT][GA]AG
402 CTTGAG
TseI 339 GCTGC G^C[AT]GC
658 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI Asp700I BstF5I DdeI DpnII
FokI MboI MnlI MseI NdeII
Sau3AI SfaNI Tru9I Tsp509I XmnI