Restriction map for
pF1KB9586
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoT22I FauI FbaI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 77 GCGG CCGC(-3/-1)
AlwI 174 GGATC GGATC(4/5)
BanI 102 GGTGCC G^G[CT][GA]CC
BbvCI 94 CCTCAGC CCTCAGC(-5/-2)
BbvI 15 GCAGC GCAGC(8/12)
BpmI 29 CTCCAG CTGGAG(16/14)
BseRI 122 GAGGAG GAGGAG(10/8)
BspMI 87 GCAGGT ACCTGC(4/8)
BsrFI 236 GCCGGT [GA]^CCGG[CT]
Cfr10I 236 GCCGGT [GA]^CCGG[CT]
Cfr13I 112 GGCCC G^G[ACGT]CC
DdeI 95 CTCAG C^T[ACGT]AG
DpnII 175 GATC ^GATC
DsaI 170 CCATGG C^C[GA][CT]GG
EarI 295 GAAGAG CTCTTC(1/4)
EcoNI 115 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 9 GATATC GAT^ATC
HaeIII 112 GGCC GG^CC
HapII 237 CCGG C^CGG
HinfI 278 GAGTC G^A[ACGT]TC
HpaII 237 CCGG C^CGG
HphI 36 GGTGA GGTGA(8/7)
Ksp632I 295 GAAGAG CTCTTC(1/4)
MaeII 395 ACGT A^CGT
MaeIII 185 GTGAC ^GT[ACGT]AC
MboI 175 GATC ^GATC
MboII 295 GAAGA GAAGA(8/7)
MseI 343 TTAA T^TAA
MslI 219 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 237 CCGG C^CGG
MwoI 146 GCAAGCAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 170 CCATGG C^CATGG
NdeII 175 GATC ^GATC
PleI 278 GAGTC GAGTC(4/5)
PstI 271 CTGCAG CTGCA^G
PvuII 97 CAGCTG CAG^CTG
Sau3AI 175 GATC ^GATC
Sau96I 112 GGCCC G^G[ACGT]CC
SfcI 271 CTGCAG C^T[GA][CT]AG
SmlI 367 CTTGAG C^T[CT][GA]AG
SphI 372 GCATGC GCATG^C
TaqI 177 TCGA T^CGA
Tru9I 343 TTAA T^TAA
TseI 15 GCAGC G^C[AT]GC
Tsp45I 185 GTGAC ^GT[GC]AC
TthHB8I 177 TCGA T^CGA
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AfaI 349 GTAC GT^AC
397 GTAC
BsiYI 57 CCATACCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
115 CCTGGGGGAGG
BslI 57 CCATACCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
115 CCTGGGGGAGG
EcoT14I 170 CCATGG C^C[AT][AT]GG
335 CCAAGG
Fnu4HI 15 GCAGC GC^[ACGT]GC
77 GCGGC
ItaI 15 GCAGC GC^[ACGT]GC
77 GCGGC
MspA1I 75 CAGCGG C[AC]G^C[GT]G
97 CAGCTG
NspI 69 ACATGC [GA]CATG^[CT]
372 GCATGC
RsaI 349 GTAC GT^AC
397 GTAC
StyI 170 CCATGG C^C[AT][AT]GG
335 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI BsaJI BssKI BstNI Cac8I
EcoRII MnlI MvaI NlaIII NlaIV
ScrFI Tsp509I TspRI