Restriction map for
pF1KB9585
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbrPI
BbvCI BciVI BclI BfaI BfrI
BglII BlnI BsaBI BsaI BsaWI
BseAI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
Bst1107I BstBI BstEII BstF5I BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI HincII HindII HindIII
HinfI HpaI HphI KpnI MaeI
MaeIII MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Tsp509I
Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AlwI 825 GATCC GGATC(4/5)
AscI 247 GGCGCGCC GG^CGCGCC
Asp700I 984 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 77 TGGCCA TGG^CCA
BbsI 792 GAAGAC GAAGAC(2/6)
BcgI 839 GCAACAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 969 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 969 GCTCAGC GC^T[ACGT]AGC
BpuAI 792 GAAGAC GAAGAC(2/6)
BsaAI 415 TACGTG [CT]AC^GT[GA]
BsmBI 892 GAGACG CGTCTC(1/5)
BsmFI 20 GGGAC GGGAC(10/14)
BspMI 771 GCAGGT ACCTGC(4/8)
BssSI 205 CACGAG CACGAG(-5/-1)
BstAPI 383 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 68 CCATGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 824 AGATCC [GA]^GATC[CT]
CelII 969 GCTCAGC GC^T[ACGT]AGC
DdeI 970 CTCAG C^T[ACGT]AG
DpnII 825 GATC ^GATC
DraI 61 TTTAAA TTT^AAA
EarI 361 CTCTTC CTCTTC(1/4)
Ksp632I 361 CTCTTC CTCTTC(1/4)
MboI 825 GATC ^GATC
MflI 824 AGATCC [GA]^GATC[CT]
MluNI 77 TGGCCA TGG^CCA
MscI 77 TGGCCA TGG^CCA
MseI 62 TTAA T^TAA
NcoI 68 CCATGG C^CATGG
NdeII 825 GATC ^GATC
NspI 28 GCATGT [GA]CATG^[CT]
PstI 937 CTGCAG CTGCA^G
Sau3AI 825 GATC ^GATC
SfcI 937 CTGCAG C^T[GA][CT]AG
SmaI 160 CCCGGG CCC^GGG
SmlI 517 CTCAAG C^T[CT][GA]AG
Tru9I 62 TTAA T^TAA
TspRI 1030 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 788 CCAAGAAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 824 AGATCC [GA]^GATC[CT]
XmaCI 160 CCCGGG C^CCGGG
XmaI 160 CCCGGG C^CCGGG
XmnI 984 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaI 160 CCCGGG C^[CT]CG[GA]G
1003 CCCGAG
AvaII 21 GGACC G^G[AT]CC
906 GGTCC
AviII 854 TGCGCA TGC^GCA
881 TGCGCA
BsgI 454 CTGCAC GTGCAG(16/14)
478 CTGCAC
BsiWI 116 CGTACG C^GTACG
611 CGTACG
BsmAI 892 GAGAC GTCTC(1/5)
32 GTCTC
BsoBI 160 CCCGGG C^[CT]CG[GA]G
1003 CCCGAG
BsrBI 651 GAGCGG CCGCTC(-3/-3)
931 GAGCGG
EagI 401 CGGCCG C^GGCCG
920 CGGCCG
EclXI 401 CGGCCG C^GGCCG
920 CGGCCG
Eco52I 401 CGGCCG C^GGCCG
920 CGGCCG
FspI 854 TGCGCA TGC^GCA
881 TGCGCA
HgaI 718 GACGC GACGC(5/10)
894 GACGC
MaeII 416 ACGT A^CGT
676 ACGT
MslI 69 CATGGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
846 CATCGCCGTG
PpuMI 20 GGGACCC [GA]G^G[AT]CC[CT]
905 AGGTCCT
PvuII 955 CAGCTG CAG^CTG
997 CAGCTG
SfaNI 879 GATGC GCATC(5/9)
194 GCATC
SgrAI 177 CGCCGGCG C[GA]^CCGG[CT]G
758 CGCCGGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI BanI BanII BbeI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI Bsp1286I BsrFI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI KasI KspI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NgoMIV
NlaIII NlaIV RsaI SacI SacII
Sau96I ScrFI SfoI StyI TaqI
TseI TthHB8I