Restriction map for
pF1KB9578
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI ApaI ApaLI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BpmI BsaAI BsaBI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Cfr13I ClaI CpoI DpnII
DraI DraII DraIII DrdI Eam1105I
EarI EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HinfI HpaI KpnI Ksp632I
MaeI MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI NciI NdeI NdeII
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI Sau3AI
Sau96I SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AfaI 498 GTAC GT^AC
AocI 224 CCTCAGG CC^T[ACGT]AGG
Aor51HI 269 AGCGCT AGC^GCT
AscI 363 GGCGCGCC GG^CGCGCC
AspHI 430 GAGCAC G[AT]GC[AT]^C
AvaI 673 CCCGAG C^[CT]CG[GA]G
BanII 651 GGGCTC G[GA]GC[CT]^C
BbsI 276 GTCTTC GAAGAC(2/6)
BlpI 666 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 666 GCTCAGC GC^T[ACGT]AGC
BpuAI 276 GTCTTC GAAGAC(2/6)
BseRI 676 GAGGAG GAGGAG(10/8)
BsiHKAI 430 GAGCAC G[AT]GC[AT]^C
BsmFI 436 GTCCC GGGAC(10/14)
BsoBI 673 CCCGAG C^[CT]CG[GA]G
BsrI 704 ACTGG ACTGG(1/-1)
BstAPI 513 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 224 CCTCAGG CC^T[ACGT]AGG
CelII 666 GCTCAGC GC^T[ACGT]AGC
Eco47III 269 AGCGCT AGC^GCT
Eco81I 224 CCTCAGG CC^T[ACGT]AGG
EcoT14I 611 CCATGG C^C[AT][AT]GG
HincII 403 GTCAAC GT[CT]^[GA]AC
HindII 403 GTCAAC GT[CT]^[GA]AC
KspI 110 CCGCGG CCGC^GG
MaeII 434 ACGT A^CGT
MaeIII 236 GTCAC ^GT[ACGT]AC
NcoI 611 CCATGG C^CATGG
NotI 115 GCGGCCGC GC^GGCCGC
PstI 511 CTGCAG CTGCA^G
RsaI 498 GTAC GT^AC
SacII 110 CCGCGG CCGC^GG
SfaNI 291 GATGC GCATC(5/9)
StyI 611 CCATGG C^C[AT][AT]GG
Tsp45I 236 GTCAC ^GT[GC]AC
TspRI 479 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 28 enzymes) #
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AlwNI 514 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
517 CAGCTGCTG
BanI 31 GGCGCC G^G[CT][GA]CC
189 GGCGCC
BbeI 31 GGCGCC GGCGC^C
189 GGCGCC
BmyI 430 GAGCAC G[AGT]GC[ACT]^C
651 GGGCTC
BsaI 201 GAGACC GGTCTC(1/5)
627 GGTCTC
BsiYI 223 CCCTCAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
419 CCACCCTTCGG
BslI 223 CCCTCAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
419 CCACCCTTCGG
Bsp1286I 430 GAGCAC G[AGT]GC[ACT]^C
651 GGGCTC
BsrBI 664 CCGCTC CCGCTC(-3/-3)
24 GAGCGG
BssKI 408 CCTGG ^CC[ACGT]GG
555 CCAGG
BstF5I 501 CATCC GGATG(2/0)
632 CATCC
BstNI 408 CCTGG CC^[AT]GG
555 CCAGG
EcoRII 408 CCTGG ^CC[AT]GG
555 CCAGG
FauI 79 CCCGC CCCGC(4/6)
663 CCCGC
FokI 501 CATCC GGATG(9/13)
632 CATCC
HphI 540 GGTGA GGTGA(8/7)
698 TCACC
KasI 31 GGCGCC G^GCGCC
189 GGCGCC
MboII 459 GAAGA GAAGA(8/7)
277 TCTTC
MvaI 408 CCTGG CC^[AT]GG
555 CCAGG
NarI 31 GGCGCC GG^CGCC
189 GGCGCC
NlaIII 532 CATG CATG^
612 CATG
NlaIV 31 GGCGCC GG[ACGT]^[ACGT]CC
189 GGCGCC
PvuII 196 CAGCTG CAG^CTG
517 CAGCTG
ScrFI 408 CCTGG CC^[ACGT]GG
555 CCAGG
SfcI 330 CTACAG C^T[GA][CT]AG
511 CTGCAG
SfoI 31 GGCGCC GGC^GCC
189 GGCGCC
TaqI 494 TCGA T^CGA
692 TCGA
TthHB8I 494 TCGA T^CGA
692 TCGA
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AcyI AluI BbvI
BglI BsaHI BsaJI BsiEI BsmAI
BsrFI BssHII BstUI Cac8I CfoI
Cfr10I DdeI DsaI EaeI EagI
EclXI Eco52I Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI MnlI MspA1I MspI
MvnI MwoI NaeI NgoMIV TseI