Restriction map for
pF1KB9575
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BanI BbeI BbrPI
BbvCI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaHI BsiEI
BsiWI BsmBI Bsp1407I BspHI BspLU11I
BspMI BsrFI BsrGI BssHII BstBI
CelII Cfr10I CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FseI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccIII 1586 TCCGGA T^CCGGA
Alw44I 1420 GTGCAC G^TGCAC
AlwNI 1453 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1420 GTGCAC G^TGCAC
Asp700I 17 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1325 TGCGCA TGC^GCA
BamHI 1589 GGATCC G^GATCC
BanII 1441 GAGCCC G[GA]GC[CT]^C
BcgI 639 CGATGGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 281 TGATCA T^GATCA
BglI 1546 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1188 CACGTA [CT]AC^GT[GA]
BsaBI 1710 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1815 GAGACC GGTCTC(1/5)
BseAI 1586 TCCGGA T^CCGGA
BsgI 967 CTGCAC GTGCAG(16/14)
BsmI 1283 GCATTC GAATGC(1/-1)
BspDI 688 ATCGAT AT^CGAT
BspEI 1586 TCCGGA T^CCGGA
BsrBI 85 CCGCTC CCGCTC(-3/-3)
BssSI 1627 CTCGTG CACGAG(-5/-1)
Bst1107I 865 GTATAC GTA^TAC
BstAPI 1230 GCATGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1535 CCATGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 865 GTATAC GTA^TAC
ClaI 688 ATCGAT AT^CGAT
EcoNI 12 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1228 ATGCAT ATGCA^T
FbaI 281 TGATCA T^GATCA
FspI 1325 TGCGCA TGC^GCA
HincII 1684 GTTGAC GT[CT]^[GA]AC
HindII 1684 GTTGAC GT[CT]^[GA]AC
MamI 1710 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1586 TCCGGA T^CCGGA
NsiI 1228 ATGCAT ATGCA^T
PleI 154 GAGTC GAGTC(4/5)
PstI 1450 CTGCAG CTGCA^G
StuI 9 AGGCCT AGG^CCT
XmnI 17 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 832 GTAGAC GT^[AC][GT]AC
865 GTATAC
AccII 79 CGCG CG^CG
1719 CGCG
AcsI 1663 GAATTT [GA]^AATT[CT]
1774 AAATTT
AocI 65 CCTCAGG CC^T[ACGT]AGG
274 CCTGAGG
ApoI 1663 GAATTT [GA]^AATT[CT]
1774 AAATTT
AspHI 109 GTGCTC G[AT]GC[AT]^C
1420 GTGCAC
AvaI 42 CCCGAG C^[CT]CG[GA]G
466 CCCGAG
BcnI 33 CCCGG CC^[GC]GG
1128 CCGGG
BsaWI 25 TCCGGT [AT]^CCGG[AT]
1586 TCCGGA
BsiHKAI 109 GTGCTC G[AT]GC[AT]^C
1420 GTGCAC
BsoBI 42 CCCGAG C^[CT]CG[GA]G
466 CCCGAG
BstUI 79 CGCG CG^CG
1719 CGCG
Bsu36I 65 CCTCAGG CC^T[ACGT]AGG
274 CCTGAGG
DraII 224 AGGACCT [GA]G^G[ACGT]CC[CT]
567 GGGACCT
DrdI 1342 GACCCACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1723 GACACCAAGGTC
EarI 616 CTCTTC CTCTTC(1/4)
1032 GAAGAG
Eco81I 65 CCTCAGG CC^T[ACGT]AGG
274 CCTGAGG
EcoO109I 224 AGGACCT [GA]G^G[ACGT]CC[CT]
567 GGGACCT
HgaI 71 GACGC GACGC(5/10)
1107 GCGTC
Ksp632I 616 CTCTTC CTCTTC(1/4)
1032 GAAGAG
MaeII 240 ACGT A^CGT
1189 ACGT
MvnI 79 CGCG CG^CG
1719 CGCG
NciI 33 CCCGG CC^[GC]GG
1128 CCGGG
NlaIV 567 GGGACC GG[ACGT]^[ACGT]CC
1589 GGATCC
PpuMI 224 AGGACCT [GA]G^G[AT]CC[CT]
567 GGGACCT
SphI 1230 GCATGC GCATG^C
1328 GCATGC
TfiI 443 GATTC G^A[AT]TC
592 GAATC
XcmI 998 CCATCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1532 CCACCATGCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BbsI BbvI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NspI PflMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII