Restriction map for
pF1KB9573
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BpmI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI HphI KasI KpnI
KspI MaeII MamI MluI MroI
MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 51 CGCG CG^CG
AlwI 533 GGATC GGATC(4/5)
AlwNI 350 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 79 GGTCC G^G[AT]CC
BalI 998 TGGCCA TGG^CCA
BbsI 726 GTCTTC GAAGAC(2/6)
BciVI 278 GGATAC GTATCC(6/5)
BcnI 76 CCCGG CC^[GC]GG
BlpI 649 GCTAAGC GC^T[ACGT]AGC
BmyI 416 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 649 GCTAAGC GC^T[ACGT]AGC
BpuAI 726 GTCTTC GAAGAC(2/6)
BsaJI 8 CCTCGG C^C[ACGT][ACGT]GG
BsiYI 15 CCCTGCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 15 CCCTGCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 80 GTCCC GGGAC(10/14)
Bsp1286I 416 GTGCCC G[AGT]GC[ACT]^C
BsrDI 575 CATTGC GCAATG(2/0)
BstF5I 553 CATCC GGATG(2/0)
BstNI 349 CCAGG CC^[AT]GG
BstUI 51 CGCG CG^CG
BstYI 533 GGATCT [GA]^GATC[CT]
Cac8I 48 GCCCGC GC[ACGT]^[ACGT]GC
CelII 649 GCTAAGC GC^T[ACGT]AGC
EaeI 998 TGGCCA [CT]^GGCC[GA]
EcoRII 349 CCAGG ^CC[AT]GG
EcoT22I 550 ATGCAT ATGCA^T
FauI 49 CCCGC CCCGC(4/6)
FokI 553 CATCC GGATG(9/13)
HapII 77 CCGG C^CGG
HgaI 52 GCGTC GACGC(5/10)
HincII 195 GTTGAC GT[CT]^[GA]AC
HindII 195 GTTGAC GT[CT]^[GA]AC
HpaII 77 CCGG C^CGG
MaeIII 1021 GTCAC ^GT[ACGT]AC
MfeI 236 CAATTG C^AATTG
MflI 533 GGATCT [GA]^GATC[CT]
MluNI 998 TGGCCA TGG^CCA
MscI 998 TGGCCA TGG^CCA
MspI 77 CCGG C^CGG
MunI 236 CAATTG C^AATTG
MvaI 349 CCAGG CC^[AT]GG
MvnI 51 CGCG CG^CG
NciI 76 CCCGG CC^[GC]GG
NlaIII 465 CATG CATG^
NsiI 550 ATGCAT ATGCA^T
PacI 934 TTAATTAA TTAAT^TAA
SfcI 959 CTACAG C^T[GA][CT]AG
SpeI 522 ACTAGT A^CTAGT
Tsp45I 1021 GTCAC ^GT[GC]AC
XcmI 1001 CCAGTGCTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 533 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AluI 804 AGCT AG^CT
1115 AGCT
BbvI 860 GCAGC GCAGC(8/12)
19 GCTGC
BfaI 523 CTAG C^TAG
689 CTAG
BsmAI 152 GTCTC GTCTC(1/5)
208 GTCTC
BsrI 996 ACTGG ACTGG(1/-1)
1001 CCAGT
BssKI 76 CCCGG ^CC[ACGT]GG
349 CCAGG
Cfr13I 12 GGCCC G^G[ACGT]CC
79 GGTCC
DpnII 503 GATC ^GATC
534 GATC
Fnu4HI 19 GCTGC GC^[ACGT]GC
860 GCAGC
ItaI 19 GCTGC GC^[ACGT]GC
860 GCAGC
MaeI 523 CTAG C^TAG
689 CTAG
MboI 503 GATC ^GATC
534 GATC
NdeII 503 GATC ^GATC
534 GATC
Sau3AI 503 GATC ^GATC
534 GATC
Sau96I 12 GGCCC G^G[ACGT]CC
79 GGTCC
ScrFI 76 CCCGG CC^[ACGT]GG
349 CCAGG
SfaNI 476 GCATC GCATC(5/9)
552 GCATC
TfiI 685 GAATC G^A[AT]TC
876 GAATC
TseI 19 GCTGC G^C[AT]GC
860 GCAGC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AcsI ApoI BseRI DdeI
EarI HaeIII HinfI Ksp632I MboII
MnlI MseI MwoI NlaIV PleI
Tru9I Tsp509I TspRI