Restriction map for
pF1KB9569
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BcnI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FseI FspI HapII
HindIII HpaI HpaII KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 1098 GAATTC [GA]^AATT[CT]
AflIII 1007 ACATGT A^C[GA][CT]GT
ApoI 1098 GAATTC [GA]^AATT[CT]
Asp700I 1179 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1865 GTGCTC G[AT]GC[AT]^C
BbsI 3 GAAGAC GAAGAC(2/6)
BclI 1233 TGATCA T^GATCA
BglII 17 AGATCT A^GATCT
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaBI 12 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1865 GTGCTC G[AT]GC[AT]^C
Bsp1407I 201 TGTACA T^GTACA
BspLU11I 1007 ACATGT A^CATGT
BspMI 557 ACCTGC ACCTGC(4/8)
BsrBI 1860 GAGCGG CCGCTC(-3/-3)
BsrGI 201 TGTACA T^GTACA
DraI 195 TTTAAA TTT^AAA
DraII 1852 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1766 CCATGG C^C[GA][CT]GG
EcoNI 94 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1852 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1098 GAATTC G^AATTC
FbaI 1233 TGATCA T^GATCA
HaeII 1527 AGCGCC [GA]GCGC^[CT]
HincII 798 GTCAAC GT[CT]^[GA]AC
HindII 798 GTCAAC GT[CT]^[GA]AC
MaeII 1552 ACGT A^CGT
MamI 12 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1766 CCATGG C^CATGG
NheI 303 GCTAGC G^CTAGC
NspI 1007 ACATGT [GA]CATG^[CT]
PpuMI 1852 GGGACCC [GA]G^G[AT]CC[CT]
SexAI 1967 ACCTGGT A^CC[AT]GGT
SspBI 201 TGTACA T^GTACA
TaqI 975 TCGA T^CGA
TthHB8I 975 TCGA T^CGA
XmnI 1179 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcyI 336 GACGCC G[GA]^CG[CT]C
624 GACGCC
AfaI 202 GTAC GT^AC
1895 GTAC
BanI 1803 GGCACC G^G[CT][GA]CC
1828 GGTGCC
BfaI 304 CTAG C^TAG
1400 CTAG
BglI 2179 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2209 GCCTCCAAGGC
BsaHI 336 GACGCC G[GA]^CG[CT]C
624 GACGCC
BstXI 62 CCAATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
156 CCAACTCGCTGG
FauI 1984 GCGGG CCCGC(4/6)
2183 GCGGG
Hin1I 336 GACGCC G[GA]^CG[CT]C
624 GACGCC
MaeI 304 CTAG C^TAG
1400 CTAG
MslI 468 CACGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1161 CATCCGTGTG
PflMI 1312 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1674 CCAGGTGATGG
PleI 1566 GAGTC GAGTC(4/5)
2143 GAGTC
RsaI 202 GTAC GT^AC
1895 GTAC
Van91I 1312 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1674 CCAGGTGATGG
XcmI 1157 CCAACATCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1670 CCAGCCAGGTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvCI BbvI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII