Restriction map for
pF1KB9568
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccI AflII AflIII AgeI AhdI
AocI AscI AseI AsnI AspEI
AviII AvrII BclI BfrI BglII
BlnI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI DraI DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV FbaI FspI
HpaI KspI MfeI MluI MunI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RsrII SacII SalI ScaI
SfuI SgrAI SnaBI SpeI SspI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 2929 GGTACC G^GTACC
Alw44I 4045 GTGCAC G^TGCAC
ApaLI 4045 GTGCAC G^TGCAC
Asp700I 4095 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2929 GGTACC G^GTACC
AspI 1482 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 889 GTCTTC GAAGAC(2/6)
BbvCI 2910 CCTCAGC CCTCAGC(-5/-2)
BciVI 3392 GTATCC GTATCC(6/5)
BfaI 3010 CTAG C^TAG
BpuAI 889 GTCTTC GAAGAC(2/6)
BsaBI 675 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2012 GAGACC GGTCTC(1/5)
BsiEI 2889 CGGCCG CG[GA][CT]^CG
BsiWI 995 CGTACG C^GTACG
BspDI 2488 ATCGAT AT^CGAT
BspHI 4310 TCATGA T^CATGA
BstAPI 671 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2488 ATCGAT AT^CGAT
EagI 2889 CGGCCG C^GGCCG
EclXI 2889 CGGCCG C^GGCCG
Eco52I 2889 CGGCCG C^GGCCG
EcoT22I 364 ATGCAT ATGCA^T
FseI 2890 GGCCGGCC GGCCGG^CC
HincII 4291 GTCAAC GT[CT]^[GA]AC
HindII 4291 GTCAAC GT[CT]^[GA]AC
HindIII 187 AAGCTT A^AGCTT
KpnI 2929 GGTACC GGTAC^C
MaeI 3010 CTAG C^TAG
MamI 675 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 258 CATATG CA^TATG
NsiI 364 ATGCAT ATGCA^T
PflFI 1482 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 4310 TCATGA T^CATGA
SbfI 3252 CCTGCAGG CCTGCA^GG
SmaI 3277 CCCGGG CCC^GGG
SphI 116 GCATGC GCATG^C
Sse8387I 3252 CCTGCAGG CCTGCA^GG
TfiI 912 GATTC G^A[AT]TC
Tth111I 1482 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3277 CCCGGG C^CCGGG
XmaI 3277 CCCGGG C^CCGGG
XmnI 4095 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AatII 763 GACGTC GACGT^C
2440 GACGTC
AccII 113 CGCG CG^CG
1062 CGCG
AclI 649 AACGTT AA^CGTT
1582 AACGTT
AcsI 2498 GAATTT [GA]^AATT[CT]
3487 AAATTT
Aor51HI 2574 AGCGCT AGC^GCT
2914 AGCGCT
ApaI 1038 GGGCCC GGGCC^C
2253 GGGCCC
ApoI 2498 GAATTT [GA]^AATT[CT]
3487 AAATTT
BamHI 1089 GGATCC G^GATCC
2127 GGATCC
BbeI 3033 GGCGCC GGCGC^C
3219 GGCGCC
BbrPI 1366 CACGTG CAC^GTG
1387 CACGTG
BsaAI 1366 CACGTG [CT]AC^GT[GA]
1387 CACGTG
BsmI 519 GAATGC GAATGC(1/-1)
3798 GCATTC
Bsp1407I 629 TGTACA T^GTACA
932 TGTACA
BsrDI 415 GCAATG GCAATG(2/0)
3932 GCAATG
BsrGI 629 TGTACA T^GTACA
932 TGTACA
BstUI 113 CGCG CG^CG
1062 CGCG
Eco47III 2574 AGCGCT AGC^GCT
2914 AGCGCT
KasI 3033 GGCGCC G^GCGCC
3219 GGCGCC
MvnI 113 CGCG CG^CG
1062 CGCG
NarI 3033 GGCGCC GG^CGCC
3219 GGCGCC
NcoI 12 CCATGG C^CATGG
2867 CCATGG
PmaCI 1366 CACGTG CAC^GTG
1387 CACGTG
PmlI 1366 CACGTG CAC^GTG
1387 CACGTG
Psp1406I 649 AACGTT AA^CGTT
1582 AACGTT
SapI 1216 GAAGAGC GCTCTTC(1/4)
4098 GCTCTTC
SexAI 146 ACCTGGT A^CC[AT]GGT
2015 ACCAGGT
SfiI 3114 GGCCTTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3250 GGCCTGCAGGGCC
SfoI 3033 GGCGCC GGC^GCC
3219 GGCGCC
SmlI 121 CTCAAG C^T[CT][GA]AG
1321 CTCAAG
SspBI 629 TGTACA T^GTACA
932 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccIII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbvI BcgI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII