Restriction map for
pF1KB9563
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccIII AclI AcsI AgeI ApoI
AscI AseI AsnI Asp700I AspI
AvrII BcgI BciVI BclI BlnI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DraI
EcoRI FbaI HpaI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SgrAI SnaBI SpeI
SphI SspBI SwaI Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AatII 6766 GACGTC GACGT^C
AflII 6943 CTTAAG C^TTAAG
AflIII 6662 ACATGT A^C[GA][CT]GT
Aor51HI 5222 AGCGCT AGC^GCT
BamHI 3305 GGATCC G^GATCC
BbrPI 2588 CACGTG CAC^GTG
BfrI 6943 CTTAAG C^TTAAG
BglII 3737 AGATCT A^GATCT
BspHI 6060 TCATGA T^CATGA
BspLU11I 6662 ACATGT A^CATGT
BsrDI 3687 CATTGC GCAATG(2/0)
BstBI 921 TTCGAA TT^CGAA
Eco47III 5222 AGCGCT AGC^GCT
EcoRV 2947 GATATC GAT^ATC
EcoT22I 6101 ATGCAT ATGCA^T
FseI 1979 GGCCGGCC GGCCGG^CC
HincII 1636 GTCAAC GT[CT]^[GA]AC
HindII 1636 GTCAAC GT[CT]^[GA]AC
MseI 6944 TTAA T^TAA
NsiI 6101 ATGCAT ATGCA^T
NspV 921 TTCGAA TT^CGAA
PmaCI 2588 CACGTG CAC^GTG
PmlI 2588 CACGTG CAC^GTG
RcaI 6060 TCATGA T^CATGA
SexAI 3857 ACCTGGT A^CC[AT]GGT
SfiI 5712 GGCCTTCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 921 TTCGAA TT^CGAA
SspI 4963 AATATT AAT^ATT
TfiI 5698 GATTC G^A[AT]TC
Tru9I 6944 TTAA T^TAA
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# TWO CUT ENZYMES ( 32 enzymes) #
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Acc65I 805 GGTACC G^GTACC
2225 GGTACC
AhdI 3524 GACAGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4507 GACTCTGGGTC
Alw44I 3280 GTGCAC G^TGCAC
3658 GTGCAC
ApaLI 3280 GTGCAC G^TGCAC
3658 GTGCAC
Asp718I 805 GGTACC G^GTACC
2225 GGTACC
AspEI 3524 GACAGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4507 GACTCTGGGTC
AviII 1388 TGCGCA TGC^GCA
4592 TGCGCA
BbeI 3877 GGCGCC GGCGC^C
4575 GGCGCC
BlpI 1381 GCTGAGC GC^T[ACGT]AGC
5754 GCTGAGC
Bpu1102I 1381 GCTGAGC GC^T[ACGT]AGC
5754 GCTGAGC
BsaAI 2588 CACGTG [CT]AC^GT[GA]
4294 TACGTG
BsmBI 301 GAGACG CGTCTC(1/5)
1089 GAGACG
BstEII 2568 GGTGACC G^GT[ACGT]ACC
2683 GGTGACC
BstPI 2568 GGTGACC G^GT[ACGT]ACC
2683 GGTGACC
CelII 1381 GCTGAGC GC^T[ACGT]AGC
5754 GCTGAGC
EagI 1978 CGGCCG C^GGCCG
3440 CGGCCG
Eam1105I 3524 GACAGCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4507 GACTCTGGGTC
EclXI 1978 CGGCCG C^GGCCG
3440 CGGCCG
Eco52I 1978 CGGCCG C^GGCCG
3440 CGGCCG
EcoO65I 2568 GGTGACC G^GT[ACGT]ACC
2683 GGTGACC
FspI 1388 TGCGCA TGC^GCA
4592 TGCGCA
KasI 3877 GGCGCC G^GCGCC
4575 GGCGCC
KpnI 805 GGTACC GGTAC^C
2225 GGTACC
NarI 3877 GGCGCC GG^CGCC
4575 GGCGCC
PaeR7I 1441 CTCGAG C^TCGAG
4030 CTCGAG
SacI 1438 GAGCTC GAGCT^C
5023 GAGCTC
SbfI 246 CCTGCAGG CCTGCA^GG
4182 CCTGCAGG
ScaI 3270 AGTACT AGT^ACT
6956 AGTACT
SfoI 3877 GGCGCC GGC^GCC
4575 GGCGCC
Sse8387I 246 CCTGCAGG CCTGCA^GG
4182 CCTGCAGG
Tsp509I 1279 AATT ^AATT
6005 AATT
XhoI 1441 CTCGAG C^TCGAG
4030 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI