Restriction map for
pF1KB9559
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AccII AccIII AhdI Alw44I ApaLI
AscI AspEI AspI AviII AvrII
BalI BbeI BbrPI BcgI BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstUI CelII CpoI
EagI Eam1105I EclXI Eco52I EcoNI
FauI FseI FspI HpaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PvuI RcaI RsrII
SacII SalI SbfI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1817 GACGTC GACGT^C
Acc65I 2257 GGTACC G^GTACC
AclI 971 AACGTT AA^CGTT
AflII 45 CTTAAG C^TTAAG
AgeI 181 ACCGGT A^CCGGT
AocI 539 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1382 AGCGCT AGC^GCT
ApaI 1437 GGGCCC GGGCC^C
Asp718I 2257 GGTACC G^GTACC
BamHI 402 GGATCC G^GATCC
BciVI 2995 GTATCC GTATCC(6/5)
BclI 2268 TGATCA T^GATCA
BfrI 45 CTTAAG C^TTAAG
BglI 1635 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3045 AGATCT A^GATCT
BsaWI 181 ACCGGT [AT]^CCGG[AT]
BsmBI 24 CGTCTC CGTCTC(1/5)
BspDI 2767 ATCGAT AT^CGAT
BsrDI 155 CATTGC GCAATG(2/0)
Bst1107I 2213 GTATAC GTA^TAC
BstEII 211 GGTGACC G^GT[ACGT]ACC
BstPI 211 GGTGACC G^GT[ACGT]ACC
BstZ17I 2213 GTATAC GTA^TAC
Bsu36I 539 CCTCAGG CC^T[ACGT]AGG
ClaI 2767 ATCGAT AT^CGAT
DraI 967 TTTAAA TTT^AAA
DraIII 2687 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1281 TGGCCG [CT]^GGCC[GA]
Eco47III 1382 AGCGCT AGC^GCT
Eco81I 539 CCTCAGG CC^T[ACGT]AGG
EcoO65I 211 GGTGACC G^GT[ACGT]ACC
EcoRI 350 GAATTC G^AATTC
EcoRV 1184 GATATC GAT^ATC
FbaI 2268 TGATCA T^GATCA
HaeII 1382 AGCGCT [GA]GCGC^[CT]
HindIII 1586 AAGCTT A^AGCTT
KpnI 2257 GGTACC GGTAC^C
NcoI 2624 CCATGG C^CATGG
NdeI 1514 CATATG CA^TATG
NheI 2191 GCTAGC G^CTAGC
PinAI 181 ACCGGT A^CCGGT
PpuMI 307 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 3241 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 971 AACGTT AA^CGTT
SacI 687 GAGCTC GAGCT^C
SfiI 1634 GGCCAGACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2588 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1943 GTCTAC GT^[AC][GT]AC
2213 GTATAC
AcyI 1817 GACGTC G[GA]^CG[CT]C
2759 GGCGTC
AseI 82 ATTAAT AT^TAAT
724 ATTAAT
AsnI 82 ATTAAT AT^TAAT
724 ATTAAT
AvaI 1657 CTCGGG C^[CT]CG[GA]G
3019 CCCGAG
BanI 2257 GGTACC G^G[CT][GA]CC
2416 GGTGCC
BanII 687 GAGCTC G[GA]GC[CT]^C
1437 GGGCCC
BbvCI 1567 CCTCAGC CCTCAGC(-5/-2)
2869 CCTCAGC
BcnI 1842 CCGGG CC^[GC]GG
2795 CCGGG
BsaHI 1817 GACGTC G[GA]^CG[CT]C
2759 GGCGTC
BsaI 184 GGTCTC GGTCTC(1/5)
573 GGTCTC
BsgI 2330 GTGCAG GTGCAG(16/14)
2392 GTGCAG
BsmI 1776 GAATGC GAATGC(1/-1)
1679 GCATTC
BsoBI 1657 CTCGGG C^[CT]CG[GA]G
3019 CCCGAG
BspLU11I 418 ACATGT A^CATGT
1736 ACATGT
BsrFI 181 ACCGGT [GA]^CCGG[CT]
1865 GCCGGT
BstXI 2201 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2999 CCATTGTACTGG
Cfr10I 181 ACCGGT [GA]^CCGG[CT]
1865 GCCGGT
DrdI 628 GACTTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3052 GACAGACTGGTC
DsaI 2624 CCATGG C^C[GA][CT]GG
2646 CCGTGG
EarI 2008 GAAGAG CTCTTC(1/4)
2291 GAAGAG
EcoT14I 2624 CCATGG C^C[AT][AT]GG
2666 CCTTGG
EcoT22I 339 ATGCAT ATGCA^T
1810 ATGCAT
HgaI 369 GCGTC GACGC(5/10)
2760 GCGTC
Hin1I 1817 GACGTC G[GA]^CG[CT]C
2759 GGCGTC
HincII 150 GTCAAC GT[CT]^[GA]AC
2119 GTCAAC
HindII 150 GTCAAC GT[CT]^[GA]AC
2119 GTCAAC
Ksp632I 2008 GAAGAG CTCTTC(1/4)
2291 GAAGAG
NciI 1842 CCGGG CC^[GC]GG
2795 CCGGG
NsiI 339 ATGCAT ATGCA^T
1810 ATGCAT
PflMI 312 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2619 CCATGCCATGG
PshBI 82 ATTAAT AT^TAAT
724 ATTAAT
PstI 1783 CTGCAG CTGCA^G
2722 CTGCAG
SapI 2008 GAAGAGC GCTCTTC(1/4)
2291 GAAGAGC
ScaI 1161 AGTACT AGT^ACT
1202 AGTACT
SmlI 45 CTTAAG C^T[CT][GA]AG
978 CTTGAG
SspI 1799 AATATT AAT^ATT
2944 AATATT
StyI 2624 CCATGG C^C[AT][AT]GG
2666 CCTTGG
Van91I 312 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2619 CCATGCCATGG
XbaI 714 TCTAGA T^CTAGA
3048 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI