Restriction map for
pF1KB9556
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# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI BamHI
BbrPI BcgI BclI BglI BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
ClaI CpoI DraIII Eam1105I Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI MamI MluI
MroI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
StuI SwaI XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 2440 GACGTC GACGT^C
AflII 2121 CTTAAG C^TTAAG
AlwI 1244 GATCC GGATC(4/5)
AlwNI 82 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1789 CCTAAGG CC^T[ACGT]AGG
ApaI 613 GGGCCC GGGCC^C
AspI 2440 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 833 TGCGCA TGC^GCA
AvrII 852 CCTAGG C^CTAGG
BalI 272 TGGCCA TGG^CCA
BbvCI 70 CCTCAGC CCTCAGC(-5/-2)
BciVI 1767 GGATAC GTATCC(6/5)
BfrI 2121 CTTAAG C^TTAAG
BglII 1376 AGATCT A^GATCT
BlnI 852 CCTAGG C^CTAGG
BlpI 2404 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2404 GCTAAGC GC^T[ACGT]AGC
BsmBI 2293 GAGACG CGTCTC(1/5)
Bsp1407I 1898 TGTACA T^GTACA
BspLU11I 2545 ACATGT A^CATGT
BspMI 2430 GCAGGT ACCTGC(4/8)
BsrBI 266 GAGCGG CCGCTC(-3/-3)
BsrDI 1859 GCAATG GCAATG(2/0)
BsrFI 423 GCCGGT [GA]^CCGG[CT]
BsrGI 1898 TGTACA T^GTACA
Bsu36I 1789 CCTAAGG CC^T[ACGT]AGG
CelII 2404 GCTAAGC GC^T[ACGT]AGC
Cfr10I 423 GCCGGT [GA]^CCGG[CT]
DrdI 860 GACCCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 573 CGGCCG C^GGCCG
EclXI 573 CGGCCG C^GGCCG
Eco52I 573 CGGCCG C^GGCCG
Eco81I 1789 CCTAAGG CC^T[ACGT]AGG
EcoNI 553 CCTGGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 833 TGCGCA TGC^GCA
HincII 492 GTTGAC GT[CT]^[GA]AC
HindII 492 GTTGAC GT[CT]^[GA]AC
KspI 439 CCGCGG CCGC^GG
MfeI 2558 CAATTG C^AATTG
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
MslI 2257 CACGCGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2558 CAATTG C^AATTG
NdeI 1583 CATATG CA^TATG
NspI 2545 ACATGT [GA]CATG^[CT]
PaeR7I 682 CTCGAG C^TCGAG
PflFI 2440 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1915 CAGCTG CAG^CTG
SacII 439 CCGCGG CCGC^GG
SapI 2158 GAAGAGC GCTCTTC(1/4)
SbfI 2427 CCTGCAGG CCTGCA^GG
Sse8387I 2427 CCTGCAGG CCTGCA^GG
SspBI 1898 TGTACA T^GTACA
SspI 1456 AATATT AAT^ATT
Tth111I 2440 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 682 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1609 GTCTAC GT^[AC][GT]AC
2041 GTCTAC
AcsI 1398 AAATTC [GA]^AATT[CT]
1921 GAATTT
AflIII 2460 ACGTGT A^C[GA][CT]GT
2545 ACATGT
ApoI 1398 AAATTC [GA]^AATT[CT]
1921 GAATTT
AvaI 472 CCCGAG C^[CT]CG[GA]G
682 CTCGAG
BbeI 253 GGCGCC GGCGC^C
1867 GGCGCC
BpmI 2174 CTCCAG CTGGAG(16/14)
2599 CTGGAG
BsaI 2602 GAGACC GGTCTC(1/5)
917 GGTCTC
BsgI 465 CTGCAC GTGCAG(16/14)
1596 CTGCAC
BsiEI 61 CGGTCG CG[GA][CT]^CG
573 CGGCCG
BsoBI 472 CCCGAG C^[CT]CG[GA]G
682 CTCGAG
BstEII 396 GGTGACC G^GT[ACGT]ACC
1743 GGTGACC
BstPI 396 GGTGACC G^GT[ACGT]ACC
1743 GGTGACC
BstYI 1243 AGATCC [GA]^GATC[CT]
1376 AGATCT
DraI 1393 TTTAAA TTT^AAA
1924 TTTAAA
DsaI 439 CCGCGG C^C[GA][CT]GG
695 CCGTGG
EaeI 272 TGGCCA [CT]^GGCC[GA]
573 CGGCCG
EcoO65I 396 GGTGACC G^GT[ACGT]ACC
1743 GGTGACC
EcoT14I 852 CCTAGG C^C[AT][AT]GG
2534 CCAAGG
HaeII 253 GGCGCC [GA]GCGC^[CT]
1867 GGCGCC
KasI 253 GGCGCC G^GCGCC
1867 GGCGCC
MflI 1243 AGATCC [GA]^GATC[CT]
1376 AGATCT
NarI 253 GGCGCC GG^CGCC
1867 GGCGCC
PflMI 81 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
382 CCAGCAAATGG
PstI 2354 CTGCAG CTGCA^G
2428 CTGCAG
SacI 676 GAGCTC GAGCT^C
2214 GAGCTC
SfoI 253 GGCGCC GGC^GCC
1867 GGCGCC
StyI 852 CCTAGG C^C[AT][AT]GG
2534 CCAAGG
Van91I 81 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
382 CCAGCAAATGG
XhoII 1243 AGATCC [GA]^GATC[CT]
1376 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I